Navigation Links
The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature

res). Detecting low-copy-number templates is especially problematic in a complex genomic DNA background (multiple false priming sites) due to frequent mispriming events at ambient temperature. In systems prone to primer-dimer formation, the synthesis of primer oligomers leads to reduced yield and obscured detection of specific product. In high-throughput applications, PCR reactions are robotically assembled in multiple 96-well dishes and then stored for varying periods of time prior to temperature cycling. Nonspecific primer extension can occur frequently during prolonged storage, leading to high background. In each of these difficult PCR systems, hot- start methods reduce background and improve detection sensitivity by preventing priming until stringent primer annealing temperatures are reached.

Extreme Specificity and Sensitivity

To demonstrate the high specificity and sensitivity of Herculase Hotstart DNA polymerase, amplicons were synthesized from limiting amounts of target DNA in the presence of denatured genomic DNA. The addition of exogenous DNA creates extremely challenging test systems for evaluating PCR enzyme specificity and sensitivity. Herculase Hotstart DNA polymerase successfully amplified a 115-bp fragment of the gag gene from as few as 5 copies of DNA template, and no extraneous bands were evident (Figure 1). In contrast, amplification with non-hot-start enzymes produces high background and low detection sensitivity.

The enhanced sensitivity of Herculase Hotstart DNA polymerase is also demonstrated in Figure 2, where a 1.7-kb fragment was amplified fr
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Comparing Fidelity and Performance of Proofreading PCR Enzymes
2. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
3. Greater Amplification Specificity with New Hot Start PCR Enzyme
4. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
5. Improve Amplification Specificity with Hot Start PCR Enzyme
6. Choice of RT-PCR Enzymes
7. Properties of PCR Enzymes
8. Select the Optimal Enzyme for RT-PCR
9. Enzyme Purification With the Econo-Pac Q Cartridge
10. Improved Sensitivity for Staining RNA with SYBR Gold Stain
11. Improved Vectors for PathDetect Trans-Reporting Systems
Post Your Comments:
(Date:10/22/2014)... Oct. 22, 2014   Synthetic Biologics, Inc. ... therapies for serious infections and diseases, announced today that ... Notice of Allowance for a composition of matter patent ... difficile program, SYN-004. This is Synthetic Biologics, first ... U.S. and adds to the Company,s extensive C. ...
(Date:10/22/2014)... and HONG KONG , Oct. ... disease therapeutics enterprise, announced today that rare disease expert ... as vice president, research. Dr. McKew brings more than ... leadership positions at the National Institutes of Health, Wyeth ... by Wyeth). Dr. McKew will lead aTyr,s efforts to expand ...
(Date:10/20/2014)... 20, 2014 Asterias Biotherapeutics, Inc. (NYSE ... signed a Notice of Grant Award (NGA) with ... October 1, 2014.  The NGA provides for the ... release of additional grant funds pursuant to the ... clinical development of Asterias, product, AST-OPC1. The grant ...
(Date:10/20/2014)... BOSTON , Oct. 20, 2014 ... commercialization company tackling big healthcare problems, announced today ... investment round, with participation from Invesco Perpetual, a ... be used to drive PureTech,s existing pipeline forward ... technologies. "PureTech has the scientific creativity to really ...
Breaking Biology Technology:Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 4aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6
... fewer than a thousand atoms, the electronic devices on semiconductor ... characterize them. Now for the first time, engineers have demonstrated ... them atom-by-atom. , ,In a study published in the Aug. ... a UW-Madison professor of electrical and computer engineering, and Keith ...
... - Gov. Jim Doyle on Monday expressed hope for ... Leader Bill Frist's recent decision to support removal of ... ,Doyle made his comments at the groundbreaking of the ... praised Wisconsin's medical development scene. , ,In remarks to ...
... Wis. - Esker Software , a provider of ... of a new mail house at its U.S. headquarters in ... Esker on Demand service. , ,The new mail processing ... in Lyon, France and its strategic partners in the U.S. ...
Cached Biology Technology:Engineers chart semiconductors on the scale of atoms 2Engineers chart semiconductors on the scale of atoms 3Engineers chart semiconductors on the scale of atoms 4Doyle praises Frist's new stem cell research position 2Doyle praises Frist's new stem cell research position 3
(Date:10/18/2014)... suspected genetic conditions, a certain type of exome sequencing ... than traditional molecular diagnostic methods, according to a study ... released to coincide with the American Society of Human ... protein­coding region of the genome (the complete set of ... organism), has been rapidly applied in research settings and ...
(Date:10/17/2014)... release is available in German . ... of sperms every day in order to reproduce? And why ... the topic of the latest issue of the research journal ... The evolutionary biologist Steven Ramm from Bielefeld University Bielefeld has ... is not unusual for a female to copulate with several ...
(Date:10/17/2014)... To date, antidotes ... doctors are often limited to supportive therapy such as induced ... of drugs involved. So what can be done if a ... professor Jean-Christophe Leroux from the Institute of Pharmaceutical Sciences at ... "The task was to develop an agent that could eliminate ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Sperm wars 2Emergency aid for overdoses 2Emergency aid for overdoses 3
... be associated with lower blood pressure (BP), and the diets ... such as diet, body weight, physical activity and alcohol intake ... modifications have been shown to be effective for preventing and ... 32 studies published from 1900 to 2013 in which participants ...
... are revealing how microbes living on floating pieces of ... potential harm they pose to invertebrates, humans and other ... into the largely unexplored world of the "Plastisphere" ... plastic that was first discovered last year. ...
... Geosphere postings cover using traditional geochemistry with ... Belt; an investigation of mafic rock samples from a ... southeastern Colorado Plateau of New Mexico and Arizona; a ... and micro-analytical techniques; and an addition to the "The ...
Cached Biology News:AGU: Uncovering the secret world of the Plastisphere 2Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 2Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 3Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 4Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 5Geosphere covers Mexico, the Colorado Plateau, Russia, and offshore New Jersey 6
... The simple three step procedure (PCR, Exonuclease ... completed in one day. The typical cloning ... 80%. , Features and Benefits: , , ... into any expression vector , High Cloning ...
Designed for use with all 96-well SPRI reagent kits. Used with round bottom 96-well microplates, and 96-well thermal cycling plates....
... be used with all 96-well ... stacking of covered plates ... with automated systems ... are available for use with ...
... This CLS number is ... to easily match Cornings product ... yet, please order under the ... contact customer service for assistance. ...
Biology Products: