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The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature



Novel Herculase Hotstart DNA polymerase results in greater specificity and sensitivity

Michael Borns Brad Scott Holly Hogrefe

Stratagene

Stratagenes Herculase DNA polymerase *, is the best enzyme for difficult PCR applications, particularly those involving long, complex, low-copy number, or GC-rich templates. We have expanded our hot-start PCR enzyme product line to include Herculase Hotstart DNA polymerase.*, This polymerase provides high specificity and detection sensitivity, while facilitating high-throughput robotic PCR applications.

Stratagene is dedicated to developing high-performance PCR enzymes that excel in both routine and demanding PCR applications. We designed the novel Herculase Enhanced DNA polymerase with the goal of realizing higher product yields over an extremely broad range of target sizes and complexities.1,2 To achieve hot-start capability, Herculase Hotstart DNA polymerase is formulated with a combination of antibodies that effectively neutralize DNA polymerase and 3'-5' (proofreading) exonuclease activities. Full enzyme activity is regained once the antibody is denatured during ramp-up to initial denaturation temperatures.

Enhanced Amplification with Hot-Start Enzymes

Amplification systems that are most likely to benefit from hot start are those designed to detect low-copy-number targets in complex DNA backgrounds, are prone to primer-dimer formation, or are assembled robotically (high-throughput PCR procedu
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