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The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using ,,, an Atmospheric Pressure Photoionization Source

hase conditions show an increase on the order of 10 to 70 times of that observed under reversed phase conditions (Figure 6).

The post-column addition of dopant flow can have an effect on the overall response. This will vary from compound to compound. As shown in Figure 7, the response of naphthalene is increased by a factor of 7, while the response of benzo(a)pyrene is relatively unchanged.

Under normal phase conditions, 5000 pg/L fell out of the calibration range for most PAHs, except Indeno(1,2,3,c,d) pyrene and Dibenz(a,h)anthracene. Reversed phase conditions could be calibrated as high as 5000 pg/L (Figure 8).

Ambient air samples were collected on high volume filters(subsequently solvent extracted, dried to 0.2 ml) from three locations, rural, urban and a traffic tunnel. Sample chromatogramsare shown in Figure 9, and calculated ambient concentrations are shown in Table 2.

Conclusions

  • The new PhotoSpray source provides a sensitive ionization technique for analysis of polycyclic aromatic hydrocarbons by LC/MS/MS.
  • Normal phase conditions provide an increase in sensitivity by a factor of at least 10 and as much as 70 over reversed phase conditions.
  • The APPI LC/MS/MS normal phase method provides adequate sensitivity to be useful in the analysis of atmospheric aerosol samples for PAHs.

References

1Robb, D. B., Covey, T. R., Bruins, A. P., Anal. Chem. 2000, 72, 3653-3659.

Authors

Gary Impey, Byron Kieser, Jean-Franois Alary, Applied Biosystems/MDS SCIEX

Acknowledgements

The authors wish to thank Satoshi Irei (York University, Toronto, Canada) for supplying PAH standards and the ambient air samples for analysis.


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