Navigation Links
The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using ,,, an Atmospheric Pressure Photoionization Source

hase conditions show an increase on the order of 10 to 70 times of that observed under reversed phase conditions (Figure 6).

The post-column addition of dopant flow can have an effect on the overall response. This will vary from compound to compound. As shown in Figure 7, the response of naphthalene is increased by a factor of 7, while the response of benzo(a)pyrene is relatively unchanged.

Under normal phase conditions, 5000 pg/L fell out of the calibration range for most PAHs, except Indeno(1,2,3,c,d) pyrene and Dibenz(a,h)anthracene. Reversed phase conditions could be calibrated as high as 5000 pg/L (Figure 8).

Ambient air samples were collected on high volume filters(subsequently solvent extracted, dried to 0.2 ml) from three locations, rural, urban and a traffic tunnel. Sample chromatogramsare shown in Figure 9, and calculated ambient concentrations are shown in Table 2.

Conclusions

  • The new PhotoSpray source provides a sensitive ionization technique for analysis of polycyclic aromatic hydrocarbons by LC/MS/MS.
  • Normal phase conditions provide an increase in sensitivity by a factor of at least 10 and as much as 70 over reversed phase conditions.
  • The APPI LC/MS/MS normal phase method provides adequate sensitivity to be useful in the analysis of atmospheric aerosol samples for PAHs.

References

1Robb, D. B., Covey, T. R., Bruins, A. P., Anal. Chem. 2000, 72, 3653-3659.

Authors

Gary Impey, Byron Kieser, Jean-Franois Alary, Applied Biosystems/MDS SCIEX

Acknowledgements

The authors wish to thank Satoshi Irei (York University, Toronto, Canada) for supplying PAH standards and the ambient air samples for analysis.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:7/10/2014)... --  Ceres, Inc . (Nasdaq: CERE ), ... results for the three months ended May 31, 2014 ... Ceres reported that the company and its customers have ... in Brazil, which concluded in June, despite dry and ... for part of the growing season. Yields of several ...
(Date:7/10/2014)... fight against terrorism with the creation of a sensor ... use of light and special glass fibres. , Published ... , the researchers describe a novel optical fibre sensor ... 6.3 ppm (parts per million). It requires an analysis ... detection has involved looking for metals that encase them ...
(Date:7/10/2014)... the journal PNAS (Proceedings of the National Academy ... and sleep deprivation have a significant effect on our ... the best time of day to test for diseases ... medicines effectively. , Researchers from the University of Surrey ... links between sleep deprivation, body clock disruption and metabolism, ...
(Date:7/10/2014)... Hall effect in bilayer graphene and shown that this ... electric field. , The fractional quantum Hall effect, ... are exposed to large magnetic fields, is a striking ... behave as a single system. However, while the basic ... of this collective behavior remain not well understood, in ...
Breaking Biology Technology:Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Detecting trace amounts of explosives with light 2Time of day crucial to accurately test for diseases, new research finds 2Columbia researchers observe tunable quantum behavior in bilayer graphene 2Columbia researchers observe tunable quantum behavior in bilayer graphene 3
... New York Times columnist David Brooks ... believes American society is in the midst of two fundamental ... information technology revolution that promotes individual power. , ,The latter ... solidarity and fraternity. , ,Brooks traveled to Madison last week ...
... Wis. - Be very afraid when a member of Congress ... is that no one else in the world does it like ... vogue, there are certain things this country does well - and ... rivals and competitors overseas. , ,One of those things is innovation. ...
... Wis. - The Wisconsin Alumni Research Foundation ... prominent technology companies, allowing them to develop and commercialize ... stem cell research. Terms of the license agreements were ... strategic collaborations between the two companies, Becton Dickinson ...
Cached Biology Technology:David Brooks: Segmented nation looks for solidarity amid IT-powered niches 2David Brooks: Segmented nation looks for solidarity amid IT-powered niches 3Changes to U.S. patent law must nurture our culture of innovation 2Changes to U.S. patent law must nurture our culture of innovation 3Changes to U.S. patent law must nurture our culture of innovation 4
(Date:7/10/2014)... BLUE BELL, Pa. , July 2, 2014  Unisys ... The Netherlands , Unisys Nederland N.V., today announced that ... (DJI) to deliver, implement and manage a new Basic Provision ... . Unisys won the contract with a ... for identity management software. The term of the contract is ...
(Date:7/10/2014)... -- Research and Markets ( http://www.researchandmarkets.com/research/h7h8lq/gesture ) has ... Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, ... to their offering http://photos.prnewswire.com/prnh/20130307/600769 ... Sensing Market to Grow Exponentially. Touch-less sensing applications ... companies were unable to leverage this technology due ...
(Date:7/10/2014)... 9, 2014  Acuity Market Intelligence today released forecasts from ... indicating that the global market for National Electronic ID (eID) ... 2018. During this time, the number of National eID cards ... Asia , with its vast population, will ... cards issued, while Europe trails a ...
Breaking Biology News(10 mins):Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2National Electronic ID Programs Generate $54 Billion Between 2013 and 2018 with 3.5 Billion National eID Card Holders Worldwide 2
... Dec. 6, 2012  Integrated Biometrics, LLC (IB) today ... of its newest finger print technology.  IB,s development ... Light Emitting Sensor (LES) technology along with a ... highly engineered charged polymer film interacting with the ...
... 5, 2012 While science has often focused on ... in the journal Bioscience suggests that long-term, ... climate change affects multiple components of ecosystem structure and ... important to understanding the effects of a changing climate ...
... In a first-of-its-kind clinical trial, physician-researchers at University ... are exploring the use of "Lose It!," a ... Principal Investigator Justin Lappen, MD, Assistant Director, Residency ... Center MacDonald Women,s Hospital, is recruiting postpartum patients ...
Cached Biology News:Integrated Biometrics Introduces Sherlock The World's Lightest, Thinnest, Smallest, Appendix F Mobile ID Fingerprint Sensors 2Site-specific, long-term research expanding understanding of climate change 2
N,N'-Methylene-bis-acrylamide, Ultra Pure...
... Serum; U.S. sourced, ideal for ... quality; Low hemoglobin; Endotoxin: < ... of US origin; U.S. sourced, ... an uncompromising quality. Low hemoglobin ...
NEWBORN CALF SERUM AND PLASMA...
... reagent for Virus isolation ... unique water insoluble Elastomeric ... and chaotropes; Engineered to ... viruses while preserving infectivity; ...
Biology Products: