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The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using ,,, an Atmospheric Pressure Photoionization Source

conditions, the performance, linearity and LOD levels were determined. The most abundant single positive transition for each PAH compound was used to construct an +MRM method. Concentrations from ambient air for several PAHs were determined from samples collected on high volume filters at both urban and rural sites. Standard solutions were prepared by serial dilution in acetonitrile/ water and iso-octane for reversed phase and normal phase conditions, respectively. Spectrometer: API 3000 LC/MS/MS System with Analyst Software PhotoSpray Source (see Figure 2 and 3) HPLC: Perkin-Elmer 200 Series Autosampler and Micro pumps

LC conditions: Acetonitrile/water (gradient mode) flow at 1.0 mL/min Column: Hypersil PAH, 100 x 4.6 mm; 5 m, 50:50 post column split Normal phase conditions: 100% iso-octane (isocratic mode) flow at 0.5 mL/min Column: Spherisorb Silica, 250 x 4.6 mm; 5 m Dopant: Toluene at a flowrate of 100 l/min.

Results

The source uses a modified housing of a conventional APCI source. The corona discharge needle has been removed and replaced with a connection for the lamp gas (nitrogen) which protects the Magnesium Fluoride optical window from the vaporised mobile phase.

Reversed phase PAH separation was accomplished by gradient flow (ACN/H2O) through a Hypersil PAH column, 100 x 4.6 mm; 5 m. Individual MRM transitions were monitored simultaneously for 16 common PAHs (see Figure 1 for corresponding PAH). Figure 4 is a 20 L injection of a 5 ng/L solution (100 ng on column).

Normal phase PAH separation was accomplished with 100% iso-octane through a Spherisorb Silica column, 250 x 4.6 mm; 5 m. Individual MRM transitions were monitored for 16 common PAHs (see Figure 1 for corresponding PAH). Figure 5 is a 20 L injection of a 5 ng/L solution (100 ng on column)

The observed response for PAHs under normal p
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