Navigation Links
The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using ,,, an Atmospheric Pressure Photoionization Source

conditions, the performance, linearity and LOD levels were determined. The most abundant single positive transition for each PAH compound was used to construct an +MRM method. Concentrations from ambient air for several PAHs were determined from samples collected on high volume filters at both urban and rural sites. Standard solutions were prepared by serial dilution in acetonitrile/ water and iso-octane for reversed phase and normal phase conditions, respectively. Spectrometer: API 3000 LC/MS/MS System with Analyst Software PhotoSpray Source (see Figure 2 and 3) HPLC: Perkin-Elmer 200 Series Autosampler and Micro pumps

LC conditions: Acetonitrile/water (gradient mode) flow at 1.0 mL/min Column: Hypersil PAH, 100 x 4.6 mm; 5 m, 50:50 post column split Normal phase conditions: 100% iso-octane (isocratic mode) flow at 0.5 mL/min Column: Spherisorb Silica, 250 x 4.6 mm; 5 m Dopant: Toluene at a flowrate of 100 l/min.

Results

The source uses a modified housing of a conventional APCI source. The corona discharge needle has been removed and replaced with a connection for the lamp gas (nitrogen) which protects the Magnesium Fluoride optical window from the vaporised mobile phase.

Reversed phase PAH separation was accomplished by gradient flow (ACN/H2O) through a Hypersil PAH column, 100 x 4.6 mm; 5 m. Individual MRM transitions were monitored simultaneously for 16 common PAHs (see Figure 1 for corresponding PAH). Figure 4 is a 20 L injection of a 5 ng/L solution (100 ng on column).

Normal phase PAH separation was accomplished with 100% iso-octane through a Spherisorb Silica column, 250 x 4.6 mm; 5 m. Individual MRM transitions were monitored for 16 common PAHs (see Figure 1 for corresponding PAH). Figure 5 is a 20 L injection of a 5 ng/L solution (100 ng on column)

The observed response for PAHs under normal p
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:12/17/2014)... (PRWEB) December 17, 2014 Based ... the overall surgical imaging market is dominated by ... the surgical imaging market. These players include GE ... Ziehm Imaging (Germany). , Full Report Copy ... of GE Healthcare can be attributed to its ...
(Date:12/17/2014)... CA (PRWEB) December 17, 2014 Once ... to provide a larger packaged waste water treatment plant ... King Abdullah Economic City (KAEC). Two years ago Bioshaft ... Jeddah Gate Development, serving two residential towers with an ... is over a quarter million gallons per day and ...
(Date:12/17/2014)... San Diego, CA (PRWEB) December 17, 2014 ... strategy and activation agency and member of the Huntsworth ... Jamie Gonzales to its executive team. In her role, ... Services. , “We are incredibly honored to welcome ... said Agency Chief and CEO, Gaëtan Fraikin. “She is ...
(Date:12/17/2014)... , Dec. 17, 2014 Tigercat ... Phase 2 study evaluating the investigational oral NK-1 ... patients with severe, chronic itch who are unresponsive ... care, including topical steroids and antihistamines. ... in Visual Analog Scale (VAS) itch score from ...
Breaking Biology Technology:Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4Audacity Welcomes Healthcare Communications Expert Jamie Gonzales as Senior Vice President of Client Services 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4
... at the American Society of Clinical Oncology (ASCO) annual meeting, ... Today at 5:00 p.m. Eastern Time - REDWOOD CITY, Calif., ... ) today announced that the QUASAR validation study met ... stage II colon cancer patients following surgery, and that the ...
... at 2:00 p.m. PT (5:00 p.m. ET)IRVINE, Calif., April 14 ... of Pulse CO-Oximetry and Measure-Through Motion and Low-Perfusion pulse oximetry, ... for the period ended April 4, 2009 after the market ... the results will begin at 2:00 p.m. PT (5:00 p.m. ...
... and New Vice President of Healthcare Brings 22 ... Ore., April 14 Prolifiq Software, Inc., today ... company as vice president of healthcare. In this ... mobile email messaging services for sales representatives in ...
Cached Biology Technology:Genomic Health Announces Positive Preliminary Results from QUASAR Validation Study of Oncotype DX(R) Colon Cancer Assay 2Genomic Health Announces Positive Preliminary Results from QUASAR Validation Study of Oncotype DX(R) Colon Cancer Assay 3Genomic Health Announces Positive Preliminary Results from QUASAR Validation Study of Oncotype DX(R) Colon Cancer Assay 4Masimo to Report First Quarter 2009 Financial Results on May 5, 2009 2Masimo to Report First Quarter 2009 Financial Results on May 5, 2009 3Prolifiq Further Expands Into Medical Markets With New Hire Maureen A. Shaffer 2Prolifiq Further Expands Into Medical Markets With New Hire Maureen A. Shaffer 3
(Date:12/5/2014)... VIEW, Calif. , Dec. 4, 2014 /PRNewswire/ ... safety has boosted investments in new testing and ... has also given rise to a range of ... by the requirements of Generation Y, which is ... technologies. As a result, product development strategies of ...
(Date:11/21/2014)... According to a new market ... RFID, Access Control, Intrusion Detection, Parking Management, Under Vehicle ... Service Geography - Global Forecasts to 2020", published by ... be around $25 Billion in 2014 and is expected ... CAGR of 8.69%. Browse 116 market data ...
(Date:11/18/2014)... 18, 2014  The Secure Identity & Biometrics ... jointly announce the formation of The Airport Entry ... Identity and Biometric Entry and Exit Solutions ... official support from BORDERPOL, the international non-profit organization ... provide expertise regarding border security, traveler and migration ...
Breaking Biology News(10 mins):Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3
... most school children understand that green plants photosynthesize, ... consider the profound global-scale effects that photosynthesis has ... light on the origins and evolution of photosynthesis ... University,s School of Life Sciences. Oxygen, one ...
... CLEVELAND Case Western Reserve University has granted ... regarding a portfolio of insulin analogs. The company must ... to obtain an exclusive license on therapies designed to ... developing treatments that it hopes will become an important ...
... discoveries about the deep ocean,s temperature variability and circulation ... The deep ocean is affected more by surface ... more accurate predictions of factors such as sea level ... temperatures have also been found to result in a ...
Cached Biology News:'Extreme' genes shed light on origins of photosynthesis 2'Extreme' genes shed light on origins of photosynthesis 3Case Western Reserve University grants option to startup Thermalin Diabetes Inc. 2New discoveries could improve climate projections 2
ID clarifier: platform...
The sealed ice cooling unit is used to absorb heat that is generated during transfers performed in the Criterion blotter....
... Tetrad 2 thermal cycler accommodates a ... heat-pump assemblies), which hold different types ... computer drives an advanced graphical interface ... lid temperatures. Peltier heat pumps, built ...
The SVT3-2 diverter valve is a 3-port, 2-position valve for use with the BioLogic DuoFlow system....
Biology Products: