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Test More siRNAs for Less


No longer must you endure the high cost and long turn-around times required for chemically synthesized siRNA. The new Silencer siRNA Construction Kit (patent pending) saves you both time and money so now you can expand your capacity for large siRNA screening experiments. More genes or more regions of the same gene can be screened for a fraction of the cost of chemically synthesized siRNA.

RNA interference (RNAi) is a powerful technique for down regulating the expression of specific genes in live cells. In mammalian cells, RNAi can be induced by transfecting small interfering RNAs (siRNAs), comprising double-stranded RNA molecules ~21 nt in length with 2 nt 3' overhangs. In order to find an siRNA with a strong effect, 35 siRNAs per gene are typically designed. This means synthesizing and purifying 610 RNA oligonucleotides. The Silencer siRNA Construction Kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. The cost and time savings gained using the Silencer siRNA Construction Kit enable screening of more genes and more potential targets within your gene to find the most potent siRNA. Higher potency siRNA translates to better signal-to-noise ratios in your experiments and fewer nonspecific effects.


How the Kit Works
Construction of an siRNA begins with the acquisition of two inexpensive, desalted DNA oligonucleotides. As illustrated in Figure 1, these oligonucletides include an 8 base sequence complementary to the 3' end of the T7 promoter primer include
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Related biology technology :

1. Silencer Validated siRNAs
2. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
3. Five Ways to Produce siRNAs
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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