Navigation Links
Test More siRNAs for Less


No longer must you endure the high cost and long turn-around times required for chemically synthesized siRNA. The new Silencer siRNA Construction Kit (patent pending) saves you both time and money so now you can expand your capacity for large siRNA screening experiments. More genes or more regions of the same gene can be screened for a fraction of the cost of chemically synthesized siRNA.

RNA interference (RNAi) is a powerful technique for down regulating the expression of specific genes in live cells. In mammalian cells, RNAi can be induced by transfecting small interfering RNAs (siRNAs), comprising double-stranded RNA molecules ~21 nt in length with 2 nt 3' overhangs. In order to find an siRNA with a strong effect, 35 siRNAs per gene are typically designed. This means synthesizing and purifying 610 RNA oligonucleotides. The Silencer siRNA Construction Kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. The cost and time savings gained using the Silencer siRNA Construction Kit enable screening of more genes and more potential targets within your gene to find the most potent siRNA. Higher potency siRNA translates to better signal-to-noise ratios in your experiments and fewer nonspecific effects.


How the Kit Works
Construction of an siRNA begins with the acquisition of two inexpensive, desalted DNA oligonucleotides. As illustrated in Figure 1, these oligonucletides include an 8 base sequence complementary to the 3' end of the T7 promoter primer include
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Silencer Validated siRNAs
2. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
3. Five Ways to Produce siRNAs
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Test More siRNAs for Less

(Date:1/15/2014)... OXFORD, England and SAN JOSE, California ... an international biotechnology company developing antibody-drug conjugates for cancer, today ... its Board of Directors. Dr Reynolds has over 20 years, ... Chief Medical Officer at Seattle Genetics. "I am ...
(Date:1/15/2014)... 2014 Two champions of science, technology, ... of an annual competition for middle and high school ... innovative STEM study. The competition presents students with real-world ... Engineering Aptitude, Mathematics, and Sciences is a program ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 AudioNotch is ... new therapy for the treatment of tinnitus. Patients listen ... frequency, and over a period of weeks to months, their ... Therapy in two forms: Notched Music and Notched White Noise. ...
(Date:1/14/2014)... 14, 2014  3D Communications, a leading provider of strategic communications services ... media events in the United States and ... Virginia Cox , JD, is returning to the firm,s ... re-joins 3D after more than two years of service as Associate ...
Breaking Biology Technology:Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Former FDA Associate Commissioner Returns To 3D Communications 2
... Ion Trap MS n , , , ... Shaler, , Paul Shieh, and William S. Hancock, , , ... Protein Glycosylation , , , , The ... mass spectrometer with MS n , capability., , , ...
... , , , , , Andreas ... , , , , ... Thermo Finnigan LCQTMion , trap mass spectrometer with MS n capability. ... , Calmodulin (CaM) is the primary high-affinity Ca ...
... , , , , , ... in eukaryotic cell cultures , requires double-stranded molecules known ... licensed supplier of siRNA, offering a custom , ... directed against common target genes, and a Cancer , ...
Cached Biology Technology:Structural Analysis of Glycosylated Peptides in Complex Mixtures with , Ion Trap MSn 2Structural Analysis of Glycosylated Peptides in Complex Mixtures with , Ion Trap MSn 3Structural Analysis of Glycosylated Peptides in Complex Mixtures with , Ion Trap MSn 4Structural Analysis of Glycosylated Peptides in Complex Mixtures with , Ion Trap MSn 5Structural Analysis of Glycosylated Peptides in Complex Mixtures with , Ion Trap MSn 6Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray , Ion Trap Mass Spectrometry 2Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray , Ion Trap Mass Spectrometry 3Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray , Ion Trap Mass Spectrometry 4Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray , Ion Trap Mass Spectrometry 5Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray , Ion Trap Mass Spectrometry 6RNAi Gene Silencing System 2RNAi Gene Silencing System 3
(Date:4/17/2014)... study suggests that fish consumption advisories for expecting ... long-lived contaminants like persistent organic pollutants (POPs). ... researchers including University of Toronto Scarborough PhD student ... how different levels of environmental contamination, a mother,s ... in the body influenced exposure in her children. ...
(Date:4/17/2014)... hundred births, Down syndrome - or trisomy 21 - ... It results from a chromosomal abnormality where cells of ... (1% of the human genome). A study conducted by ... Genetic Medicine and Development at the University of Geneva ... shed light on how the extra chromosome 21 upsets ...
(Date:4/17/2014)... in bone marrow need to produce hydrogen sulfide in ... to a new study from the Center for Craniofacial ... of USC. , Professor Songtao Shi, principal investigator on ... by the cells governs the flow of calcium ions. ... that results in osteogenesis, or the creation of new ...
Breaking Biology News(10 mins):Fish consumption advisories fail to cover all types of contaminants 2Trisomy 21: How an extra little chromosome throws the entire genome off balance 2Trisomy 21: How an extra little chromosome throws the entire genome off balance 3
... The apparently random self-assembly of molecular threads into the ... frantic than previously thought, Michigan State University scientists say. ... the nature of some diseases. How proteins spontaneously ... wide variety of functional or malfunctioning three-dimensional ...
... A phylogenetic analysis can take a long time to ... efficiently and effectively. The National Institute for Mathematical and ... focusing on high performance computing (HPC) for phylogenetics. ... to use TeraGrid, the CIPRES Portal, the iPlant Discovery ...
... The American Physical Society (APS) announces a new public ... public libraries in the United States full use of ... back to the first issue in 1893, a collection ... provide this access at no cost to participating public ...
Cached Biology News:Scientists post lower speed limit for cell-signaling protein assembly 2
Hybridization chamblers suitable for use with all ChIP-GLAS microarrays....
Rabbit polyclonal to BCA1 ( Abpromise for all tested applications). Antigen: Generated using E.coli expressed mouse BCA1. Entrez GeneID: 10563 SWISS Protein ID: O43927...
SH3 Proteins II Sampler Kit 10g each...
Tumor Suppressor Sampler Kit 10 mu g each...
Biology Products: