Navigation Links
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

Bio-Rad Laboratories, Paul Zoller, Theresa Redila-Flores, Dan Chu, and Asmita Patel, Ph.D.


Introduction
As the number of diseases and disorders linked to gene mutations grow, the need for scanning single-base mutations becomes ever more vital to extending understanding of disease-risk predictor mutations and their outcome. Since most mutations are single base changes, a number of methods that are faster and more economical than DNA sequencing have been developed. Single stranded conformation polymorphism (SSCP),1 denaturing gradient gel electrophoresis (DGGE),2 constant denaturing gel electrophoresis (CDGE),3 temperature gradient gel electrophoresis (TGGE),4 heteroduplex analysis (HA),5 protein truncation test (PTT),6 and enzymatic/chemical cleavage analysis, are the major detection methods used to screen mutations.

The DCode universal mutation detection system (Figure 1) can be used to screen mutations by any of the techniques mentioned above and by a new technique called temporal temperature gradient gel electrophoresis (TTGE).

Temporal temperature gradients previously described by Yoshino et al. and Wiese et al., have been used for electrophoretic separations where the spatial temperature gradient is replaced with a temporal temperature gradient.7,8

In TTGE, the denaturing environment is formed by a constant concentration of denaturant in the gel in combination with the temporal temperature gradient. During electrophoresis, the temperature is increased gradually and uniformly. This results in a linear temperature gradient over the course of the run (Figure 2). Electrophoretic mob
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
2. Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
3. New Temperature Cycler Features Interchangeable Blocks
4. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
5. Using Gradient PCR to determine the optimum annealing temperature.
6. Two-step Gradient PCR*
7. Mastercycler Gradient Quick Programming Guide
8. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
9. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
10. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
11. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
Post Your Comments:
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... Fertility Associates of Memphis has announced ... , a network of more than 20 contributing ... have fast access to a national pool of frozen ... Fertility Associates of Memphis is the only fertility clinic ... , Dr. William Kutteh , director of Fertility ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one of the nation’s ... Pharm.D. to the newly created position of Vice President ... Dr. Maroulis will continue to manage the entire specialty ... pharmacies as the company expands. Dr. Maroulis has been ... has served in many different capacities in the pharmacy, ...
(Date:12/19/2014)... , Dec. 19, 2014 Bina Technologies, Inc. ... announced today that they have been acquired by Roche ... is a privately held company that provides a big ... generation sequencing (NGS) data for the academic and translational ... Sequencing Unit, and will continue to focus on development ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... Mass.--A mat of nanowires with the touch and feel of paper ... and other organic pollutants, MIT researchers and colleagues report in the ... they have created a membrane that can absorb up to 20 ... for future use. The oil itself can also be recovered. Some ...
... Holliday Updates Investors on Growth Opportunities, NEW ... today, DuPont Chairman and CEO Charles O. Holliday, ... innovation that addresses,global macro trends related to food, ... a market-driven science company," Holliday said at the,Sanford ...
... By Improving the Accuracy of Tumor Grading, MapQuant Dx(TM) Genomic ... Chemotherapy Treatments per Year ... 2008 - booth 23128, CHICAGO, May 30 Ipsogen ... that markets molecular diagnostic assays,for leukaemia announced today that it ...
Cached Biology Technology:MIT develops a 'paper towel' for oil spills 2DuPont Well Positioned for Long-Term Growth in Agricultural Productivity, Safety and Security, Renewable Energy 2DuPont Well Positioned for Long-Term Growth in Agricultural Productivity, Safety and Security, Renewable Energy 3Ipsogen Launches MapQuant Dx(TM) Genomic Grade Test to Identify Breast Cancer Patients who Benefit From Chemotherapy 2Ipsogen Launches MapQuant Dx(TM) Genomic Grade Test to Identify Breast Cancer Patients who Benefit From Chemotherapy 3Ipsogen Launches MapQuant Dx(TM) Genomic Grade Test to Identify Breast Cancer Patients who Benefit From Chemotherapy 4
(Date:12/11/2014)... , Dec. 10, 2014  That blood pressure plays a ... while. Hypertension – the medical term for high blood pressure ... 1800s, and the inflatable cuff that,s used in measuring blood ... mean there,s nothing new about hypertension, its triggers and its ... about the condition and the best ways to treat it. ...
(Date:12/10/2014)... 2014 You,ve been here before: you desperately need ... your password, site key or the answer to your secret ... teacher? Today, Hoyos Labs , a ... will finally put an end to the frustration that comes ... . 1U leverages a user,s smartphone to acquire his or ...
(Date:12/10/2014)... 9, 2014  Wake Forest Baptist Medical Center today announced ... School of Medicine. Funding for this $50 million capital ... will be publicly launched next summer. ... former 60 series R.J. Reynolds Tobacco Company complex, adjacent ... begin immediately with plans to be ready to welcome ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... and accumulating calcium carbonate, and this is essential for ... An international research team has discovered that the amount ... is now significantly below rates measured over recent geological ... 70% lower. Coral reefs form some of ...
... end of the last Ice Age, trees have had thousands ... will forests adapt to the rapid pace of current climate ... grant to Drs. Stephen Keller, Andrew Elmore, Matthew Fitzpatrick, David ... for Environmental Science,s Appalachian Laboratory to study climate adaptation in ...
... observational technique have determined the precise configuration of humulones, ... flavor. That might not sound like a big ... results reported in scientific literature in the last 40 ... diabetes, some types of cancer and other maladies. ...
Cached Biology News:New evidence highlights threat to Caribbean coral reef growth 2New evidence highlights threat to Caribbean coral reef growth 3New study will predict how trees will adapt to rapid climate change 2Beer's bitter compounds could help brew new medicines 2
MOUSE ANTI SIMIAN VIRUS 5 Immunogen: Simian virus 5...
MOUSE ANTI SIMIAN VIRUS 5:FITC Immunogen: Simian virus 5...
MOUSE ANTI HUMAN MBP (67-74) Immunogen: Human myelin basic protein...
... Purification Kit is designed to purify double-stranded DNA ... synthesis and PCR. The process is simple and ... 3 g can be efficiently purified and concentrated. ... DNA is bound to the Microfilter Cartridge in ...
Biology Products: