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Technical Specifications,,, Accelerating Your Research with the BD FACSCanto,,, High Throughput Sampler Option

Technical Specifications
Accelerating Your Research with the BD FACSCanto
High Throughput Sampler Option

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Introduction

The new BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition. It is designed to speed through a microtiter plate in 15 minutes* with sample carryover tolerances of 1% or less. The BD HTS adapts to the BD FACSCanto flow cytometer within a few hours, making it a fully modular system. It supports standard 96-well U-, V-, and flat-bottom, and 384-well flat bottom plates.

Fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample injection, and probe washing. You can choose a standard throughput mode to allow for acquisition of larger sample volumes. All sampler functions are operated through BD FACSDiva software, allowing you to create customized delivery protocols with user-defined mixing, wash, and analysis parameters.

For more information about the BD FACSCanto High Throughput Sampler option or other quality products from BD Biosciences, please contact your local sales representative or call one of the locations listed on the back.

Physical Characteristics

Size
9.5 x 17.5 x 8 inches (W x D x H) (24.1 x 44.5 x 20.3 cm)
Requires 10 inches (25 cm) of table space in front of the instrument

Weight
23 lb (12.7 kg)

Noise level
Idle mode <60dBA
Run mode <75dBA

Environmental conditions
18-30 C 15-70% relative humidity

Power
100-240VAC

Fluidics

Plate/tube compatibility
The HTS option allows quick conversion between tubes and plates.

Sampling modes
High-throughput (HT) Standard throughput (STD)
If desired, the sample introduction can be temporarily switched to manual mode for loading tubes.

Sampling volume (range)
2-10 uL in HT mode
2-200 uL in STD mode

Total aspirated volume
22 uL in HT mode
22-200 uL in STD mode

Cleaning cycles
Automated cleaning protocols

Sheath, waste, and cleaning reservoirs
The HTS integrates into the BD FACSCanto fluidics cart

Recommended sheath solution
BD FACS sheath solution with surfactant (Cat. No. 346524; Europe 336911)

Performance

Throughput
<15 min in HT mode using a 2 sec acquisition
<44 min in STD mode using a 10 sec acquisition

Carryover
<1%

User-adjustable settings
Sample volume
Samples of each well are mixed prior to acquisition
Mixing volume 5-100 uL
Mixing speed 25-250 uL/sec
Number of mixes 0-5
Probe wash volume 200-800 uL
Sample volume 2-10 uL in HD mode
Sample volume 2-200 uL in STD mode

Computer Compatibility

Compatible software
BD FACSDiva software version 5.0 or higher

Compatible computer
BD PC workstation (RAM 2GB) Windows XP (SP2) and Windows 2000 (SP4)

Data management
Integ rated with BD FACSDiva version 5.0 software (available May 2006)

Keyword display in BD FACSDiva software

Compatible with Waters NuGenesis SDMS (optional)

Compatible BD Falcon Microtiter Plates
96-well U bottom (Cat. No. 353910)
96-well V bottom (Cat. No. 353263)
96-well flat bottom (Cat. No. 353915) |
384-well flat bottom (Cat. No. 353233)

Regulatory certifications
UL, CE, CSA
For research use only. Not for use in diagnostic or therapeutic procedures.

Service Support
Depot repair service (where available)

* Based on a 2-sec acquisition in HT mode
In high-throughput mode
Carryover based on testing performed with beads. Other sample types may require optimization of HTS parameters.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale.


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