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Taq DNA Polymerase

enzyme is ideal for:
  • Routine amplification of single-copy genes from eukaryotic genomes
  • Labeling of PCR products with modified nucleotides (DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Prevention of carry-over contamination, by incorporation of dUTP and degradation of contaminating DNA with Uracil-DNA Glycosylase
  • Cycle sequencing
(see also "Not all Taq DNA polymerase preparations are the same") Application profile
Operating parameters
  • pH optimum: approx. 9 (adjusted at 20C)
  • Temperature optimum (for elongation): approx. 75C
    Note: The DNA polymerase has a half life at 95C of approx. 40 min
  • Divalent ion requirement: Mg2+ (standard concentration, 1.5 mM)
  • dNTP requirement: approx. 200 M for each dNTP
Effect of additives Tween 20 (0.51.0%) detergent has been used to enhance the efficiency of Taq DNA Polymerase in certain PCRs. Other additives reported to have enhancing effects include DMSO, gelatine, glycerol, betaine, spermidine, T4 gene 32 protein, BSA, and ammonium sulfate. Experimental result Five different lots of Taq DNA Polymerase have been tested in PCR to amplify a 0.5 kb fragment of lambda DNA. Reliable, consistent results were obtained with every lot of Roche Applied Science Taq DNA Polymerase that was tested.
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MarrowMAX Bone Marrow Medium...
Sprague-Dawley rats, 7-8 weeks old, mixed gender; tissue is collected fresh, frozen on dry ice within minutes of sacrifice and stored at -20C; individual tissue weight approx. 0.05 gram....
CMRL Medium-1066 (1X) liquid...
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