Navigation Links
Taq DNA Polymerase

for routine amplification of up to 3 kb genomic DNA targets Cat. No. 1 647 679 250 units (1 unit/l)

Cat. No. 1 647 687 4 x 250 units (1 unit/l)
Cat. No. 1 146 165 100 units (5 units/l)
Cat. No. 1 146 173 500 units (5 units/l)
Cat. No. 1 418 432 4 x 250 units (5 units/l)
Cat. No. 1 596 594 10 x 250 units (5 units/l)
Cat. No. 1 435 094 20 x 250 units (5 units/l) Description Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM. It is now supplied as a recombinant enzyme from E. coli. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA Polymerase consists of a single polypeptide chain with a molecular weight of approximately 95 kD. It is a highly processive 5'3' DNA polymerase which lacks 3'5' exonuclease activity.

Note: For additional convenience and reliable PCR with minimum reagent preparation, we offer the PCR Master [Cat. No. 1636103, 2x conc. PCR reaction mix (Taq DNA Polymerase, reaction buffer with MgCl2, stabilising detergent, dNTPs)] plus water PCR grade for dilution. Further the PCR Core Kit (Cat. No. 1578553), containing Taq DNA Polymerase, dNTP stock solution, PCR reaction buffer with and without MgCl2 and a MgCl2 stock solution. Application Amplifies genomic targets up to 3 kb with good yield and specificity. Capable of amplifying lambda-DNA up to 10 kb. The enzyme is ideal for:
  • Routine amplification of single-copy genes from eukaryotic genomes
  • Labeling of PCR products with modified nucleotides (DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Prevention of carry-over contamination, by incorporation of dUTP and degradation of contaminating DNA with Uracil-DNA Glycosylase
  • Cycle sequencing
(see also "Not all Taq DNA polymerase preparations are the same") Application profile
Operating parameters
  • pH optimum: approx. 9 (adjusted at 20C)
  • Temperature optimum (for elongation): approx. 75C
    Note: The DNA polymerase has a half life at 95C of approx. 40 min
  • Divalent ion requirement: Mg2+ (standard concentration, 1.5 mM)
  • dNTP requirement: approx. 200 M for each dNTP
Effect of additives Tween 20 (0.51.0%) detergent has been used to enhance the efficiency of Taq DNA Polymerase in certain PCRs. Other additives reported to have enhancing effects include DMSO, gelatine, glycerol, betaine, spermidine, T4 gene 32 protein, BSA, and ammonium sulfate. Experimental result Five different lots of Taq DNA Polymerase have been tested in PCR to amplify a 0.5 kb fragment of lambda DNA. Reliable, consistent results were obtained with every lot of Roche Applied Science Taq DNA Polymerase that was tested. Key advantages
  • High PCR yield, because it is stable during prolonged, repetitive high temperature incubations
  • Enhances specificity of amplification, because it is highly processive and free of exonuclease and nicking activities
  • Increases sensitivity, because it produces good yields even when template amounts are limiting
  • Best results, because it has proved superior to competitors Taq DNA Polymerase preparations (see "Not all Taq DNA polymerase preparations are the same")

'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. Pfu DNA Polymerase as a Hot Start Alternative
3. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
4. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
5. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
6. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
7. High-Fidelity PCR with a Novel Polymerase Mixture
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
10. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
11. HotStarTaq DNA Polymerase
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. ... Cancer Research, London (ICR) and University ... SKY92, SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma ... MUK nine . The University of Leeds ... partly funded by Myeloma UK, and ICR will perform the ...
(Date:10/10/2017)... ... 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, has ... The bold new look is part of a transformation to increase awareness, appeal to ... period. , It will also expand its service offering from its signature gourmet cooking ...
(Date:10/10/2017)... 10, 2017 International research firm Parks Associates announced ... at the TMA 2017 Annual Meeting , October 11 in ... residential home security market and how smart safety and security products impact ... Parks Associates: Smart Home ... "The residential security market has ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
Breaking Biology Technology:
(Date:3/28/2017)... , March 28, 2017 The ... Hardware (Camera, Monitors, Servers, Storage Devices), Software (Video Analytics, ... Region - Global Forecast to 2022", published by MarketsandMarkets, ... 2016 and is projected to reach USD 75.64 Billion ... and 2022. The base year considered for the study ...
(Date:3/24/2017)... 24, 2017 The Controller General of Immigration from ... Abdulla Algeen have received the prestigious international IAIR Award for the ... Continue Reading ... ... Controller Abdulla Algeen (small picture on the right) have received the IAIR ...
(Date:3/23/2017)... The report "Gesture Recognition and Touchless Sensing Market by Technology (Touch-based ... to 2022", published by MarketsandMarkets, the market is expected to be worth USD ... 2022. Continue Reading ... ... ...
Breaking Biology News(10 mins):