Navigation Links
TTGE on the DCode System

ramped on the DCode system during the run from 6368 C, giving a temperature ramp rate of 1.7 C/hr. By using these conditions, the problem with determining the exact conditions is eliminated. Another example of the advantage of TTGE over CDGE and DGGE is shown for the KRAS exon 1 gene in Figure 2AD.

Since the denaturing conditions in TTGE span a wider range than CDGE, several fragments with different melting behavior, that are analyzed on separate gels by CDGE, can be analyzed on the same TTGE gel. This is illustrated in Figure 3, where exons 59 and 11 of the TP53 gene are analyzed. Using two different denaturing conditions and the same temperature ramp allowed all of the exons to be analyzed at one time.

These examples demonstrate that the TTGE technique is more robust and flexible than either DGGE or CDGE in mutation screening and represents a new development with advantages for mutation screening in a diagnostic setting.


Conclusion
With TTGE, the high reproducibility in casting a constant denaturant gel and the rapid and high throughput of the analyses obtained by CDGE are achieved. The problems in determining the exact denaturing conditions for fragments with only one melting domain are eliminated. The focusing of bands obtained by DGGE seems to be achieved, and since the denaturing conditions in TTGE span a wider range, several fragments with different melting behavior can be analyzed on the same gel.


References
1. Fisher, S. G. and Lerman, L. S., Proc. Nat. Acad. Sci. USA, 80, 15791583 (1983).

2. Hovig, E., et al., Mutation Research, 262, 6371 (1991).

3. Brresen, A.-L., et al., Proc. Acad. Sci. USA, 88
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
8. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
9. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
10. Detection of Mutations in the CYP21 Gene Using the DCode System
11. Heteroduplex Analysis Using the DCode System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: TTGE the DCode System

(Date:5/23/2013)... , May 23, 2013  BioScrip, Inc. (NASDAQ: ... will present at the Jefferies 2013 Global Healthcare Conference ... Hyatt New York. Richard Smith ... Hai Tran , Chief Financial Officer, will ... Eastern Daylight Time.   About BioScrip ...
(Date:5/23/2013)...  Rahal Letterman Lanigan Racing (RLL) and StemCyte, Inc., ... banking and therapeutics, announced today that StemCyte has become ... Indy car driven by James Jakes ... IndyCar 2013 season.   StemCyte, Inc. and Rahal ... of umbilical cord blood banking and its educational initiative ...
(Date:5/23/2013)... Virobay, Inc. today announced that their development ... important milestone, as Virobay has now initiated a Phase ... S inhibitor.   The first Phase 1 ... designed to evaluate the safety, tolerability, pharmacokinetics and pharmacodynamics ... "The initiation of this Phase 1 trial with ...
(Date:5/23/2013)... Washington, DC (PRWEB) May 23, 2013 ... African-Americans in STEM industries, Black Data Processing Associates (BDPA), ... Day on June 8, 2013. This all-day event will ... arena, and learn the ins and outs from many ... to 3 p.m. at the New Jersey Institute of ...
Breaking Biology Technology:StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 2StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 3Virobay, Inc. initiates a Phase 1 Trial of VBY-036, a compound intended for the treatment of neuropathic pain 2Virobay, Inc. initiates a Phase 1 Trial of VBY-036, a compound intended for the treatment of neuropathic pain 3Black Data Processing Associates (BDPA) New Jersey Hosts 10th Annual Families in Technology Day June 8, 2013 2
... to generate energy is a signifiant step closer following ... East Anglia (UEA). Published today by the leading ... Sciences (PNAS), the research demonstrates for the first ... enable bacterial cells to transfer electrical charge. The ...
... WASHINGTON, May 23, 2011The Annual Meeting of the American ... June 2, 2011, in New Orleans, Louisiana. Crystallography is ... in regular crystalline solids and in complicated molecules. The ... the structure of important materials are often calculated by ...
... (OTCQX: DATA), a technology and services company focused on ... announced the Company,s selection as a 2011 Crain,s Leading ... "Being once again recognized as an EDGE ... and its employees have to bringing a quality product ...
Cached Biology Technology:Discovery opens the door to electricity from microbes 2Sulfates in extreme places, DNA tied in knots and magnetic cooling at crystallography meeting 2Sulfates in extreme places, DNA tied in knots and magnetic cooling at crystallography meeting 3Sulfates in extreme places, DNA tied in knots and magnetic cooling at crystallography meeting 4DATATRAK Recognized as 2011 Crain's Leading EDGE Award Winner 2
(Date:5/24/2013)... years, microRNAs (miRNAs) and other non-coding RNAs are ... specific proteins. In recent years they have emerged ... to establish tissue origin for cancers of unknown ... screen for disease, but clinically tractable, diagnostic methods ... not currently available. In this issue of the ...
(Date:5/23/2013)... , Automated conserved noncoding sequence ... evolution among grasses , Within the genome of ... that undergo little change in position and sequence over ... proteins. Some of these evolutionarily stable sequences, so-called conserved ... of other genes or the condensation of chromosomes, but ...
(Date:5/23/2013)... Investigators at Rollins School of Public Health at ... Institute of Technology, have received a $4 million ... Center at Emory University (Health and Exposome Research ... first exposome-based center grant awarded in the United ... by the National Institute of Environmental Health Sciences ...
Breaking Biology News(10 mins):Frontiers news briefs: May 23 2Frontiers news briefs: May 23 3Emory, Georgia Tech receive first human exposome center grant in US 2Emory, Georgia Tech receive first human exposome center grant in US 3
... is a strong candidate for involvement in premenstrual dysphoric ... flux in hormones during the menstrual cycle. In a ... National Academy of Sciences , Rockefeller University researchers detail ... of the gene increases anxiety, dampens curiosity and tweaks ...
... has long been a model system for studying and understanding ... that cause influenza, measles and rabies. More ... be genetically modified to serve as an anti-cancer agent, exercising ... and as a potent vaccine against HIV. ...
... , REDWOOD CITY, Calif. ... leader in fingerprint identity and authentication solutions, continues to ... and franchise owners.  The latest version of the popular ... U.are.U® fingerprint biometrics technology from DigitalPersona, will be unveiled ...
Cached Biology News:Research identifies gene with likely role in premenstrual disorder 2Research identifies gene with likely role in premenstrual disorder 3Researchers reveal 3-D structure of bullet-shaped virus with potential to fight cancer, HIV 2Digital Dining Customers Demand Fingerprint Biometrics in Point-of-Sale Technology 2Digital Dining Customers Demand Fingerprint Biometrics in Point-of-Sale Technology 3
Immunogen: Synthetic peptide (KLH conjugated): L(506) S K S S S Q I R M S G L P A D N L A T(525) Storage: -20 C, Avoid Freeze/Thaw Cycles...
Polypropylene test tube racks, used in Thermo Forma's line of Test Tube Rack holders...
Attachments: Clip bar for tubes 10 to 19 mm in diameter Overall Dimension Height IC: 6 (15.2) Overall Dimension Length IC: 18.5 (46.9) Overall Dimension Width IC: 4 (10.2)...
For quantitative determination of Aldolase in Serum (an indicator of muscle damage). Ready-to-use liquid stable reagent. Formulated to eliminate interference from pyruvates. Application sheets are ...
Biology Products: