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Abstract
Using high purity Type B silica, Tosoh has introduced two new columns
with differing surface treatment procedures and carbon content levels
to provide unique selectivities for a wide range of sample types.
Introduction
In recent years, many new octadecylsilane (ODS) chemistries have been
made commercially available that offer optimized properties for select
subsets of compound types. The overriding trends in the release of new
stationary phases has shifted toward 1) high purity, metal free silica
2) base-deactivated surfaces 3) stronger retention of polar compounds
and 4) improved reproducibilities from column to column and silica lot
to silica lot. Rarely, have new ODS column types provided a universal
solution to the multitude of applications being performed. Further, column
selection to match the best column with the application has been difficult.
With the introduction of TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatography and selectivity can be achieved with a wide range of sample types. Due to the fact that both stationary phases use the same ultra-pure base silica with 5m particle size and 100 pores, the same chromatographic properties are achievable for columns of different lots. Different selectivities result by varying the carbon content and surface optimization. Table 1 summarizes these differences.
TSKgel ODS-100V columns are optimized for polar compounds while the TSKgel
ODS-100Z are the better choice for more hydrophobic compounds. Both column
types provide symmetrical peak shapes for strongly basic and acidic compounds,
demonstrating the absence of accessible
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Source:
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