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TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds


Abstract
Using high purity Type B silica, Tosoh has introduced two new columns with differing surface treatment procedures and carbon content levels to provide unique selectivities for a wide range of sample types.


Introduction
In recent years, many new octadecylsilane (ODS) chemistries have been made commercially available that offer optimized properties for select subsets of compound types. The overriding trends in the release of new stationary phases has shifted toward 1) high purity, metal free silica 2) base-deactivated surfaces 3) stronger retention of polar compounds and 4) improved reproducibilities from column to column and silica lot to silica lot. Rarely, have new ODS column types provided a universal solution to the multitude of applications being performed. Further, column selection to match the best column with the application has been difficult.

With the introduction of TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatography and selectivity can be achieved with a wide range of sample types. Due to the fact that both stationary phases use the same ultra-pure base silica with 5m particle size and 100 pores, the same chromatographic properties are achievable for columns of different lots. Different selectivities result by varying the carbon content and surface optimization. Table 1 summarizes these differences.


TSKgel ODS-100V columns are optimized for polar compounds while the TSKgel ODS-100Z are the better choice for more hydrophobic compounds. Both column types provide symmetrical peak shapes for strongly basic and acidic compounds, demonstrating the absence of accessible
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