Navigation Links
TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds


Abstract
Using high purity Type B silica, Tosoh has introduced two new columns with differing surface treatment procedures and carbon content levels to provide unique selectivities for a wide range of sample types.


Introduction
In recent years, many new octadecylsilane (ODS) chemistries have been made commercially available that offer optimized properties for select subsets of compound types. The overriding trends in the release of new stationary phases has shifted toward 1) high purity, metal free silica 2) base-deactivated surfaces 3) stronger retention of polar compounds and 4) improved reproducibilities from column to column and silica lot to silica lot. Rarely, have new ODS column types provided a universal solution to the multitude of applications being performed. Further, column selection to match the best column with the application has been difficult.

With the introduction of TSKgel ODS-100V and TSKgel ODS-100Z, excellent chromatography and selectivity can be achieved with a wide range of sample types. Due to the fact that both stationary phases use the same ultra-pure base silica with 5m particle size and 100 pores, the same chromatographic properties are achievable for columns of different lots. Different selectivities result by varying the carbon content and surface optimization. Table 1 summarizes these differences.


TSKgel ODS-100V columns are optimized for polar compounds while the TSKgel ODS-100Z are the better choice for more hydrophobic compounds. Both column types provide symmetrical peak shapes for strongly basic and acidic compounds, demonstrating the absence of accessible residual silanol groups and metal-ion impurities on the silica surface, respectively.


Results
Basic and Acidic Compounds:
The tricyclic antidepressant desipramine, containing a secondary amine group, was analyzed at pH 7.0 to illustrate column performance under conditions where accessible residual silanol groups would be negatively charged. It is apparent from Figure 1 that tailing is minimized on new TSK-GEL ODS columns relative to other commercially available C18 column brands. Similarly, at pH 2.2, formic acid used as the test probe to judge the chromatographic behavior of charged acidic compounds, reveals good peak shape as indicated in Figure 2 .



Hydrophobic and Polar Compounds:
The hydrophobicity and surface polarity of both stationary phases are represented in Figure 3 . The TSKgel ODS-100V has more polar properties than most recently released AQ-type phases. Retention of hydrophobic test probes differing in one or more methylene groups, indicated that TSKgel ODS-100Z has more hydrophobic character than TSKgel ODS-100V and is comparable to the majority of commercially available phases. Thus, scientists can use standard mobile phases when testing the performance of the new TSK-GEL ODS columns in comparison to brands they currently use.


Reproducibility:
Lot-to-lot reproducibility of the TSKgel ODS-100Z is shown in Figure 4 . A total of six different stationary phases lots were examined. The six lots included 3 different base silica lots, thus representing columns from different silica and bonding lots, a nd columns that differ only in the bonding lot. Consistent chromatographic results were obtained regardless of base silica or bonding lot.



Conclusions:

The recently commercialized TSKgel ODS-100V and TSKgel ODS-100Z column set provides a universal solution for most reverse phase separations.


More Infomation
TSKgel ODS-100V
TSKgel ODS-100Z



'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
2. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
3. Three Micron TSK-GEL ODS-100V Columns for High Throughput LCMS
4. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
5. New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
6. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
7. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
8. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
9. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
10. Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
11. Self-Drying Vacuum Pumps... the solution for applications producing condensation
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/24/2016)... ... June 24, 2016 , ... Researchers at the Universita ... miRNAs in people with peritoneal or pleural mesothelioma. Their findings are the subject of ... now. , Diagnostic biomarkers are signposts in the blood, lung fluid or tissue ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is pleased to announce the launch of their brand, UP4™ Probiotics, into Target ... over 35 years, is proud to add Target to its list of well-respected ...
(Date:6/23/2016)... , June 23, 2016 /PRNewswire/ - FACIT ... Ontario biotechnology company, Propellon Therapeutics ... development and commercialization of a portfolio of first-in-class ... Epigenetic targets such as WDR5 represent an exciting ... significantly in precision medicine for cancer patients. Substantial ...
(Date:6/23/2016)... , June 23, 2016 Houston ... with the Cy-Fair Sports Association to serve as ... the agreement, Houston Methodist Willowbrook will provide sponsorship ... and connectivity with association coaches, volunteers, athletes and ... with the Cy-Fair Sports Association and to bring ...
Breaking Biology Technology:
(Date:6/7/2016)... TORONTO , June 7, 2016  Syngrafii ... begun a business relationship that includes integrating Syngrafii,s ... pilot branch project. This collaboration will result in ... for the credit union, while maintaining existing document ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/2/2016)... --  The Weather Company , an IBM Business (NYSE: ... in which consumers will be able to interact with IBM ... voice or text and receive relevant information about the product ... have long sought an advertising solution that can create a ... and valuable; and can scale across millions of interactions and ...
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of ... the latest premium product recently added to the range of products distributed by Ampronix. ... ... ... Medical Display- Ampronix News ...
Breaking Biology News(10 mins):