Navigation Links
TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

interaction sites with another SH2-containing protein.8

This approach is superior to other methods: A number of known candidate molecules can be quickly and simultaneously screened by immunoblot analyses and be readily modified to identify novel proteins through the use of labeled cellular extracts. Fusion proteins that contain a single tyrosine residue can be used to map sites of interaction; and interaction sites can be identified which, when mutated in vivo, lead to a loss of protein activity or stability. For example, when two tyrosine residues mutate within the juxtamembrane region of the platelet-derived growth factor receptor, the kinase is rendered catalytically inactive;9 hence, interacting proteins cannot be identified.

Additional Applications

Use the tyrosine-phosphorylated fusions for a variety of applications: Carry out direct interactions with soluble tyrosine-phosphorylated proteins and immobilized interaction SH2/PTB domains, both produced in E. coli; screen bacteriophage expression libraries (for a nonisotopic variation of the CORT assay,10 in which novel SH2-containing proteins are cloned using a carboxy-terminal region of receptor tyrosine kinases); and generate and purify tyrosine-phosphospecific antibodies.

Alternatively, phosphotyrosine-containing peptides that are chemically synthesized can also be used to yield similar results. However, synthetic peptide reagents are expensive, nonrenewable, and require prior immobilization for such an assay (e.g., biotinylation or chemical crosslinking to a support matrix). On the other hand, tyrosine-phosphorylated fusion proteins produced in bacteria are a renewable and inexpensive resource.

Conclusions

Tyrosine phosphorylated and unphosphorylated polypeptides are efficiently produced in TKB1 and BL21 bacteria, respectively. These proteins can be used to rapidly define a
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
6. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
7. Simple Isolation of RNA from Tissue and Cultured Cells
8. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
(Date:5/21/2013)... of a new study led by George Washington ... that nucleation of ice in small droplets is ... behavior at the nanoscale. The formation of ice ... research question whose answer also has important implications ... The crystallization of ice from supercooled water is ...
(Date:5/21/2013)... , May 21, 2013 The ... and Transplant Association) is the biggest congress in ... therapy. Nearly 10,000 participants are in Istanbul ... latest research findings. New pioneering studies have been presented: ... FERRIC PYROPHOSPHATE (SFP) ADMINISTERED VIA HEMODIALYSATE REDUCES ESA USE" ...
(Date:5/21/2013)... 21, 2013 Clinverse, Inc. ... for clinical trials, announced today it has formed ... Clinverse’s ClinPay® FLS eClinical commerce platform aggregates ... SpendMD ™, Aggregate Spend Solutions’ recently-launched ... tracking and reporting transfer of value at investigator ...
(Date:5/21/2013)... ALBANY, New York , May 21, 2013 /PRNewswire/ ... published by Transparency Market Research ( http://www.transparencymarketresearch.com ) "Biochip ... Science Research, IVD Tests) - U.S. Industry Analysis, Size, Share, ... USD 2.4 billion in 2011 and is expected to ... 2018, to reach an estimated value of USD 4.7 ...
Breaking Biology Technology:Study led by GW professor provides better understanding of water's freezing behavior at nanoscale 2ERA-EDTA CONGRESS 2013: Late Breaking Clinical Trials 2ERA-EDTA CONGRESS 2013: Late Breaking Clinical Trials 3Clinverse, Inc. Announces Strategic Alliance with Aggregate Spend Solutions, LLC 2Clinverse, Inc. Announces Strategic Alliance with Aggregate Spend Solutions, LLC 3The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 2The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 3The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 4The U.S. Biochip Products and Services Market is Expected to Reach USD 4.7 Billion in 2018: Transparency Market Research 5
... , PHILADELPHIA and LONDON, July 22 ... compendium of pharmaceutical research and development statistics, the CMR International ... offers extended content including patent information and emerging trends in ... R&D Factbook provides the data that R&D companies need ...
... , CHICAGO, July 22 Research presented ... an assay developed by Abbott for simultaneous detection of both HIV antigens ... this study compared to HIV antibody-only assays. Earlier detection was shown ... , "The ability to use a blood test to diagnose ...
... , , ROCKVILLE, Md., ... ) today announced that the U.S. Government has exercised its option ... Strategic National Stockpile, to be delivered over a three-year period, beginning ... $151 million from this award as deliveries are completed. ...
Cached Biology Technology:Thomson Reuters Announces 2009 Edition of the CMR International Pharmaceutical R&D Factbook 2Abbott HIV Test Demonstrates Earlier Disease Detection 2Abbott HIV Test Demonstrates Earlier Disease Detection 3Human Genome Sciences Announces New Order for Raxibacumab (ABthrax(TM)) from U.S. Government 2Human Genome Sciences Announces New Order for Raxibacumab (ABthrax(TM)) from U.S. Government 3Human Genome Sciences Announces New Order for Raxibacumab (ABthrax(TM)) from U.S. Government 4Human Genome Sciences Announces New Order for Raxibacumab (ABthrax(TM)) from U.S. Government 5Human Genome Sciences Announces New Order for Raxibacumab (ABthrax(TM)) from U.S. Government 6
(Date:5/20/2013)... compound abundant in the Mediterranean diet takes away cancer ... a very specific step in gene regulation, this compound ... as scheduled. , One way that cancer cells thrive ... to die on a regular cycle that is subject ... Ohio State University researchers, found that a compound in ...
(Date:5/20/2013)... New Geology articles posted online ... cover a wide swath of geoscience subdisciplines, including ... and paleobotany. Locations studied include Siberia; the Sumatran ... Apuane, Italy; Ukraine; Mars; and the Southeastern U.S. ... Rubies, jadeite, and plate tectonics;, 2. The clear ...
(Date:5/18/2013)... AGA Research Foundation is honored to announce the first ... Cancer, which will support Andrew D. Rhim, MD, from ... Pennsylvania, Philadelphia, as he furthers his research on the ... progression. , "The AGA Research Foundation is humbled to ... and AGA Legacy Society members," said Nicholas F. LaRusso, ...
Breaking Biology News(10 mins):The compound in the Mediterranean diet that makes cancer cells 'mortal' 2The compound in the Mediterranean diet that makes cancer cells 'mortal' 3New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 2New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 3New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 4New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 5New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 6New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 7New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 8New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 9New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 10New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More 11AGA Research Foundation grant furthers digestive cancer research 2
... lines specifically tailored to match the nuclear DNA of patients, ... or spinal cord injury. , The research is being released ... non-profit science society, on the Science Express website on 19 ... of human disease in cells in the laboratory. The work ...
... of Science holds mixed blessings for scientists who follow ... study, conducted by scientists from Cincinnati Children's Hospital Medical ... Germany, reveals important evidence that tells us more about ... hand, scientists offer caution in regards to the side ...
... study has discovered that two chemical compounds may help ... without invasive gene therapy. Presented March 5 at the ... demonstrates that the new chemicals activate telomerase -- a ... them to continue destroying HIV-infected cells. , "The immune ...
Cached Biology News:Scientists generate patient-specific stem cells, Science study says 2Scientists generate patient-specific stem cells, Science study says 3Scientists generate patient-specific stem cells, Science study says 4Science study holds implications for gene therapy and stem cell biology 2Science study holds implications for gene therapy and stem cell biology 3Two chemicals boost immune cells' ability to fight HIV without gene therapy 2
CELLine System cultivation flask (for secreted products)...
... The mini incubation trays ... used to screen antigens that ... membrane. Each tray has eight ... channels that accommodate strips cut ...
... Plasma Immunodepletion Kit specifically removes 20 of ... or serum in preparation for further proteomics ... the plasma proteome whether you use one- ... or go straight to mass spectrometry. This ...
... first siRNA sequence design software in 2001, ... tools consistent with new experimental strategies and ... RNAi. With the introduction of our ... OligoEngine is pleased to offer the most ...
Biology Products: