Navigation Links
TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

3). The phosphoproteins found in the TKB1 cells expressing gT-F3 likely represented contaminating bacterial proteins, as these have not been repeatedly observed in other experiments; this suggests that TKB1 efficiently induces the tyrosine phosphorylation of GST fusion proteins but does not phosphorylate GST itself.

Figure 3

Fusion Proteins Used to Purify Signaling Molecules

As we previously determined that SHC proteins bind directly to tyrosine D on Neu (Y1227),4,8 we sought to determine whether these fusion proteins could be used to affinity purify cellular signaling molecules in vitro. Lysates from exponentially growing NIH-3T3 fibroblasts were incubated with gT-YC, gT-YD, gT-YE, and gT-F3 purified from BL21 or TKB1; specifically associated proteins were analyzed by immunoblotting with SHC specific antisera (Figure 3). SHC did not interact with fusion proteins produced in the BL21 strain, yet efficiently bound gT-YD when expressed in TKB1. The lack of binding can neither be attributed to the amounts nor the level of tyrosine phosphorylation of the fusion proteins (Figure 3, upper and middle panels). Thus, because this approach is specific, it permits rapid screenings for interactions with known proteins.

TKB1 Cells: A Superior Approach

GST-fusion proteins that harbor tyrosine phosphorylation sites can be used to affinity purify cellular signaling proteins in vitro when purified from TKB1 bacteria. These in vitro interactions are highly specific, as demonstrated by the binding of SHC protein to the same site as observed in vivo. We failed to detect proteins that do not interact with these phosphorylation sites (e.g., Ras-GAP) and successfully used this approach to map
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
6. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
7. Simple Isolation of RNA from Tissue and Cultured Cells
8. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
(Date:7/10/2014)... Canada (PRWEB) July 10, 2014 ... present in very low abundance and are often ... challenging and time-consuming. , Join presenters Dr. Rowel ... and Dr. John Anders, Head of Quality at ... spectrometer-based approach that can speed detection and quantitation ...
(Date:7/10/2014)... Cleveland, Ohio (PRWEB) July 10, 2014 ... in ion channel-expressing cell lines, and the gap that ... new cells that expand its industry-leading portfolio of validated ... focuses on ion channel-expressing cell lines, as ion channels ... able to control this activity creates the potential to ...
(Date:7/10/2014)... Francisco, CA (PRWEB) July 10, 2014 ... family closer together. The Archer Family purchased a ... contained within DNA, but ended up with a lifetime ... discoveries contained within the family members’ DNA genuinely brought ... had decided to search deeper into genetic history and ...
(Date:7/10/2014)... OMICS Group’s 5th International conference and ... 2014 at Double Tree by Hilton Beijing, China is ... Analytical & Bioanalytical research methods only to facilitate improved ... occasion, Dr. Srinubabu Gedela, MD of OMICS Group Inc ... remarkable one in bringing a unique and international mix ...
Breaking Biology Technology:Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4
... 24 Nektar Therapeutics,(Nasdaq: NKTR ) ... quarter and,year ended December 31, 2008. , ... for Nektar," said Howard W. Robin,President and Chief ... polymer,conjugation chemistry platform, we advanced numerous proprietary drug,candidates ...
... European Commission has granted,marketing authorisation for Servier,s Valdoxan(R) ... of adult patients,with major depressive episodes. , ... for improved therapies to treat the,devastating effects of ... Kingdom. "Valdoxan is an innovative, new approach to,the ...
... Gene Network Sciences, Inc. (GNS) today announced that it ... Officer of Stromedix, Inc. and former Executive Vice President ... Directors. Dr. Gilman has been Founder and Chief ... was previously Executive Vice President, Research at Biogen Idec. ...
Cached Biology Technology:Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 2Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 3Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 4Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 5Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 6Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 7Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 8Nektar Therapeutics Reports Fourth Quarter and Year-End 2008 Financial Results 9Valdoxan(R) Granted Marketing Authorisation for the Treatment of Major Depressive Episodes in Europe 2Valdoxan(R) Granted Marketing Authorisation for the Treatment of Major Depressive Episodes in Europe 3Gene Network Sciences Announces Michael Gilman as Board Member 2
(Date:7/11/2014)... 11, 2014 Research and Markets ... Technology Market - Industry Analysis Size Share Growth Trends ... http://photos.prnewswire.com/prnh/20130307/600769 ... created a need for high level security in both ... authentication such as username and passwords, tokens etc. are ...
(Date:7/11/2014)... Brazil- In the brains of all vertebrates, information ... an electric or chemical signal to be passed ... which are the most abundant type of synapse, ... is crucial for learning, memory, perception and cognition, ... critical for brain function. For instance, every ...
(Date:7/10/2014)... , June 27, 2014  The American ... National Institute of Standards and Technology (NIST) and ... its academic research and forensic science expert members ... Board is an element of the NIST,s Organization ... are among the first appointments made to the ...
Breaking Biology News(10 mins):International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2Blame it on the astrocytes 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3
... Feb. 13, 2013 AFCEA,s twelfth annual Homeland ... the Nation Safe", will be hosted at the ... , February 26-28, 2013.  Attendees include government and ... operations, policy and procurement. Additionally, the Conference will ...
... of seven well-studied ant species are opening up ... look at molecular mechanisms that underlie the complex ... Florida carpenter ant, Camponotus floridanus, has revealed a ... how the expression or suppression of particular genes ...
... shows that busy beavers are helping Canada geese get an earlier ... Ponds in Alberta where beavers were active tended to result in ... at reproductive success, according to the study, published recently in ... beavers to early season nesting habits of Canada geese in a ...
Cached Biology News:AFCEA's Homeland Security Conference Returns To Washington, D.C. 2Epigenetics shapes fate of brain vs. brawn castes in carpenter ants 2Epigenetics shapes fate of brain vs. brawn castes in carpenter ants 3Epigenetics shapes fate of brain vs. brawn castes in carpenter ants 4
Request Info...
... DNA preparations are prepared from customers ... 1mg to multi-gram scales. Each preparation ... and glycerol stocks are maintained to ... selection (Clonal selection includes screening colonies ...
... preparations are prepared from customers high-copy ... to multi-gram scales. Small-scale (1-100mg) ... are made according to customer specifications. ... to customer needs, and glycerol stocks ...
... for automated gigaseal patch clamping. The ... ion channels, such as hERG and ligand-gated ... four pipette heads afford more efficient assays ... discovery. The glass-coated tunnels of the ...
Biology Products: