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Synthesize High Yields of Biotinylated aRNA

higher concentrations both the noise increases and the aRNA yield decreases.

Ambion realizes that microarray users may prefer to continue using their own biotin-labeling method, or to experiment with different labeling conditions. To accommodate this we offer two Biotin-UTP nucleotides (Biotin-11-UTP and Biotin-16-UTP) separately, which can be used with the standard MessageAmp II Kit.


MessageAmp II-Biotin Enhanced Kit Format

The MessageAmp II-Biotin Enhanced Kit provides enough reagents for 20 single round amplification reactions. It can be used in combination with the MessageAmp II Kit for small samples that require 2 rounds of amplification (MessageAmp II for the first round and MessageAmp II-Biotin Enhanced in the second round for labeling). Biotin-UTP is provided premixed with the other rNTPS for convenience and consistent, optimized labeling.


Scientific Contributors

Robert Setterquist, Mike Wilson, Charles Johnson, Shika Agarwal, Sharmili Moturi Ambion, Inc.


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