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Synthesize High Yields of Biotinylated aRNA

MessageAmp II-Biotin Enhanced: A Single Round aRNA Amplification Kit
Ambion now offers an improved MessageAmp II Kit with biotin-11-UTP. MessageAmp II-Biotin Enhanced provides a complete single round amplification kit for biotin array analysis. The biotin modified UTP and ratio of labeled to unlabeled UTP were selected based on superior performance and aRNA yield. The biotinylated nucleotide is supplied as a pre-mixed NTP at an optimized ratio, providing convenient, consistent labeling.

Highest amplification in a single roundup to 10,000-fold for most samples

Enough aRNA for Affymetrix GeneChip analysis from just 50 ng total RNA

Optimized NTP mix containing biotinylated UTP reduces set-up time and maximizes labeling

Reduce costs by 45%


T7 RNA Polymerase Linear Amplification is the Gold Standard

Microarrays measure the expression levels of thousands of genes. One limitation of this technology, however, is the need for large amounts of labeled target RNA for hybridization to the microarray. This limitation is overcome by using a T7 linear amplification protocol commonly referred to as the Eberwine Method. This method most accurately maintains the initial mRNA expression profile and has been the gold standard for microarray target preparation [1]. Biotin, incorporated as biotinylated nucleotide tri
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