Navigation Links
Superior Gene Silencing Using Adenoviral Vectors


Mediating RNA interference (RNAi) with recombinant adenovirus expressing siRNAs has only recently been described in the literature (1). Recombinant adenoviruses have several advantages over other methods of nucleic acid delivery. Namely, these viruses can infect dividing and non-dividing cells, and can be used in animal model systems. They can infect many difficult-to-transfect primary and transformed cell lines via their receptor mediated delivery mechanism. The sequence of a chemically synthesized siRNA capable of gene specific silencing can also be functionally expressed from recombinant adenovirus, often at a lower cost. Once made, the virus can be expanded, making it possible to generate "stocks" for future experiments.


How Does pSilencer Adeno 1.0-CMV Work?
An oligonucleotide insert encoding the desired siRNA is cloned into the pre-linearized shuttle vector (provided), which includes a modified CMV promoter for efficient expression of the siRNA sequence. The shuttle vector, containing the desired sequence, is then linearized and introduced into HEK 293 packaging cells (not included) along with the linearized adenovirus backbone (Figure 1). The HEK 293 packaging cells contain the EIA and EIB genes which are not present in the introduced vectors and are required to form infectious particles. Inside the HEK 293 cells, the shuttle vector and the adenoviral backbone plasmid recombine to produce an adenoviral genome including the siRNA expression cassette. The siRNA expression cassette is packaged into infectiou
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
2. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. RNAi Gene Silencing System
6. Quantification of siRNA Silencing Efficiency Using the LightCycler System
7. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
8. Drive Long-Term Silencing with CMV Vectors
9. Enhanced siRNA Delivery and Long-term Gene Silencing
10. Fast, Accurate Assessment of siRNA-induced Gene Silencing
11. Your Data: Silencing Hepatitis C Virus Replication With In Vitro Transcribed siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Superior Gene Silencing Using Adenoviral Vectors

(Date:8/26/2014)... , Aug. 26, 2014  ViveBio, LLC a ... collection, stabilization and transportation, announced today that it ... Services, LLC (LPS) of Knoxville, Tennessee ... in the United States . ... of ambient temperature dried biological specimen transport systems.  ...
(Date:8/26/2014)... Scranton, PA (PRWEB) August 26, 2014 ... San Jose, CA, which specializes in chemical process equipment, ... with Michigan State University’s Facility for Rare Isotope Beams ... ft. long precision cleaning line made with Vycom’s Flametec ... resonators, which are the essential parts of FRIB’s linear ...
(Date:8/26/2014)... 2014  Guardant Health, a healthcare company focused ... products, has been recognized today by the World ... . As an innovator in non-invasive, tumor profiling, ... a wide variety of industries and regions to ... ® , Guardant Health,s first commercially available product, ...
(Date:8/26/2014)... 26, 2014 After searching for ... research facilities , the University of Kansas Medical ... have chosen iLab Solutions' web-based core facility management ... , "We are very pleased that the ... Center signed on with us,” stated Heather Lorenz, ...
Breaking Biology Technology:ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 3Guardant Health Named a 2015 Technology Pioneer by World Economic Forum 2The University of Kansas Medical Center and The University of Kansas Cancer Center Select iLab Solutions Web-based Software to Streamline Operations 2The University of Kansas Medical Center and The University of Kansas Cancer Center Select iLab Solutions Web-based Software to Streamline Operations 3
... 2008 DaVita Inc., a,leading provider of kidney care ... today announced its former Chief Medical,Officer (CMO) Charles J. ... stepped down as CMO in July 2007, and has ... CMO., (Logo: http://www.newscom.com/cgi-bin/prnh/20020729/DAVITALOGO ), "Charlie has ...
... Kees Bald has demonstrated that hydrogen can be efficiently ... more easily used in mobile applications. Bald discovered that ... make the favourable extraction and storage of hydrogen possible. ... storage and transport medium for energy. Therefore many future ...
... Genaera,Corporation (Nasdaq: GENR ) today announced its financial ... net loss for the year ended,December 31, 2007 was $15.7 ... a net loss of $21.2 million, or $(1.46) per share,basic ... net loss for,the quarter ended December 31, 2007 was $5.8 ...
Cached Biology Technology:DaVita Announces Retirement of Former Chief Medical Officer Charles J. McAllister, M.D. 2Genaera Corporation Announces 2007 Financial Results 2Genaera Corporation Announces 2007 Financial Results 3Genaera Corporation Announces 2007 Financial Results 4Genaera Corporation Announces 2007 Financial Results 5Genaera Corporation Announces 2007 Financial Results 6
(Date:8/26/2014)... sciences at New Jersey Institute of Technology (NJIT), is ... that sets the pace for daily life. , Evolution ... mammals with fundamental rhythms of life that include the ... with seasonal change. The brain,s circadian clock controls hormone ... when disrupted by experiences such as jet lag or ...
(Date:8/26/2014)... researchers at the University of Illinois at Urbana-Champaign ... design, paving the way for power-on-a-chip applications. ... microscopic scale that amplify light and produce ultra-narrowband ... of electrical and computer engineering (ECE) at Illinois. ... optical power on a chip containing both electronic ...
(Date:8/26/2014)... has recommended investing in dog owner education and facilities as ... obesity in both people and their pets. , In a ... that access to dog-friendly walking environments and better education about ... and take more exercise with their pets. , It is ... for a walk. In the UK, almost a quarter of ...
Breaking Biology News(10 mins):Researching fundamental rhythms of life 2Researching fundamental rhythms of life 3Symphony of nanoplasmonic and optical resonators produces laser-like light emission 2Education and dog-friendly neighbourhoods could tackle obesity 2
... by intestinal worms a condition that affects more than ... immune system is more versatile than has long been thought. ... exploration in the search for treatments against autoimmune diseases like ... tissues. The findings, reported by scientists who performed the ...
... Forest Service Southern Research Station (SRS) scientists and partners ... faster than expected in the southern Appalachians and rapidly ... and cooperators from the University of Georgia published the ... Ecosystems . "The study marks the ...
... has found that modern lifestyle habits may play a bigger ... A review of the scientific evidence over the ... good oral hygiene and health education, may override the effects ... online in a Supplement to the journal Obesity Reviews ...
Cached Biology News:A worm-and-mouse tale: B cells deserve more respect 2A worm-and-mouse tale: B cells deserve more respect 3Study finds hemlock trees dying rapidly, affecting forest carbon cycle 2
streptavidin, Alexa Fluor® 750–allophycocyanin conjugate (Alexa Fluor® 750–allophycocyanin streptavidin) *1 mg/mL*...
AedestaTM Animal Organ/Tissue Perfusion Soln....
Reusble. Uses in conjunction with the Apoptotic Cell Isolation Kit....
EGTA, 10 g. Ethylene glycol-O,O-bis-[2-amino-ethyl]-N,N,N,N,-tetraacetic acid.Assay: > 99.0%. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Biochemicals ....
Biology Products: