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SuperFect Transfection Reagent

agent is suitable for transient and stable transfection of a broad range of cell lines (see figure "Transfection of Neuronal PC-12 Cells Using SuperFect Reagent"). A searchable list of cell lines successfully transfected using SuperFect Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site www.qiagen.com/transfectiontools/.

Transfection of Neuronal PC-12 Cells Using SuperFect Reagent


Expression of green fluorescent protein (GFP) in differentiated PC-12 cells 5 days post-transfection. 104105 cells previously stimulated with 50 ng/ml NGF were plated per 60 mm dish one day prior to transfection. Transient transfections were performed in 2 ml low-serum growth medium (DMEM plus 0.05% FBS) using 3 g of a GFP-reporter plasmid and 15 l SuperFect Reagent. (Data kindly provided by K. Kelly-Spratt, University of Texas Southwestern Medical Center, Dallas, TX, USA.)

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using SuperFect Reagent requires minimal handling, making it highly suitable for high-throughput screening. SuperFect Reagent provides high transfection efficiencies, excellent reproducibility, and low cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Cited References

1. Tang, M.X., Redemann, C.T., and Szoka, Jr., F.C. (1996) In vitro gene delivery by degraded polyamidoamine dendrimers. Bioconjugate Chem. 7, 703.



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