Navigation Links
SuperFect Transfection Reagent

osome, leading to pH inhibition of endosomal nucleases, which ensures stability of SuperFectDNA complexes.

Due to highly controlled chemical synthesis the activated-dendrimer molecules in SuperFect Reagent have a precise size and a defined shape. This ensures consistent transfection-complex formation and reproducible transfection results.

Activated-Dendrimer Structure

Schematic representation of an activated dendrimer (1). Note the highly branched structure.
SuperFectDNA Interaction
Model of the SuperFectDNA complex. SuperFect Reagent (purple balls) interacts with DNA (black) to form a ring-like (toroid-like) structure. The upper right section of the illustration shows naked DNA, the lower section shows the interaction between dendrimers and DNA inside the complex, and the upper left section shows the final complete coverage of DNA within the complex. Procedure

SuperFect Transfection Reagent is provided as a ready-to-use solution. The reagent is simply added to the DNA solution, mixed, and incubated for 510 minutes to allow SuperFectDNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. After a 23 hour incubation, a medium change is performed and the cells are incubated for expression of the transfected gene.

Applications

SuperFect Re
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Efficient Transfection of Neurospora Crassa
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. Efficient Transfection of Neurospora Crassa
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: SuperFect Transfection Reagent

(Date:8/29/2014)... York (PRWEB) August 29, 2014 According to ... Orthobiologics Market” the global orthobiologics market was valued at USD ... market worth USD 5,519.9million in 2019at a CAGR of 5.9% ... aged 50 years and above is afflicted by bone and ... by the year 2020. This has and will continue to ...
(Date:8/29/2014)... Due to a misstatement posted by the ... Stem Cell Technology Center, LLC ( ASCTC ) was reported ... research that might benefit ALS patients. Because of ASCTC's ... cell technologies and therapeutic applications, FRC listed ASCTC as one ... donors who wish not to support research that requires the ...
(Date:8/28/2014)... August 28, 2014 Best Sanitizers, Inc., ... an alcohol-based hand sanitizer, asks food processors and food ... the hand sanitizer they’re currently using to Best Sanitizers’ ... hygiene is critical to fighting cross-contamination and the spread ... Sanitizers believes there are key criteria that make a ...
(Date:8/28/2014)... 28, 2014 The ability for organizations ... that will allow individuals to perform at the highest ... for employers looking to compete in this post–recession era. ... this trend, as the sector increasingly focuses on the ... which will have real results on the organization's development ...
Breaking Biology Technology:Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 2Global Orthobiologics Market to Reach $5,519.9 Million by 2019: Transparency Market Research 3The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 2The Adult Stem Cell Technology Center, LLC Declines ALS Ice Bucket Challenge Donors Wishing to Support Ethical Research for New Stem Cell Therapies 3Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3
... Years Recognizes Breakthrough ... of Heart Disease, ABBOTT PARK, Ill., Oct. 28 ... Innovation Award for its XIENCE V(TM),Everolimus Eluting Coronary Stent ... data, the next-generation drug eluting stent,represents a significant advance ...
... Oct. 28 Volcano Corporation,(Nasdaq: VOLC ), a ... the diagnosis and treatment of coronary and peripheral artery,disease, ... for the,third quarter and first nine months of fiscal ... a conference call to discuss its financial,results and operating ...
... 28 Sirnaomics, Inc., (http://www.sirnaomics.com ) announced ... SBIR,(small business innovation research) grant from National ... Sirnaomics, Inc., a,biopharmaceutical company founded in early ... Technology for novel targeted therapeutic,development. The company,s ...
Cached Biology Technology:Abbott's XIENCE V(TM) Drug Eluting Stent Wins Prestigious Chicago Innovation Award 2Abbott's XIENCE V(TM) Drug Eluting Stent Wins Prestigious Chicago Innovation Award 3Volcano Corporation Schedules Third Quarter Conference Call, Webcast 2Sirnaomics Receives SBIR Grant from NCI For Its Multi-Targeted Cancer siRNA Therapeutics 2Sirnaomics Receives SBIR Grant from NCI For Its Multi-Targeted Cancer siRNA Therapeutics 3
(Date:8/29/2014)... the repeated climatic shifts over the last 120,000 ... diversity, a team of researchers led by City ... developed a new biodiversity metric called "phylogeographic endemism." ... genetic variation within species is restricted in geographical ... biology, and 14 other researchers from institutions in ...
(Date:8/29/2014)... in German . ... natural nitrogen cycle on Earth and in biological wastewater ... to depend on nitrite as their source of energy. ... a microbiologist at the University of Vienna, has now ... alternative source of energy. The oxidation of hydrogen with ...
(Date:8/29/2014)... the prolific bloomer Emiliania huxleyi , can grow ... finding contradicts the common view that E. huxleyi ... supplies of thiamine in the ocean to survive. , ... ocean," says CIFAR Senior Fellow Alexandra Worden , ... CIFAR fellows John Archibald (Dalhousie University), ...
Breaking Biology News(10 mins):CCNY team defines new biodiversity metric 2Hydrogen powers important nitrogen-transforming bacteria 2Hydrogen powers important nitrogen-transforming bacteria 3Not all phytoplankton in the ocean need to take their vitamins 2Not all phytoplankton in the ocean need to take their vitamins 3
... keeper of the world,s shark attack records, is also now ... the sawfish. Distinguished by a long rostrum or "saw" ... its name, the sawfish has become a historical and cultural ... ichthyologist and curator of both the International Shark Attack File ...
... Geosphere , The Geological Society of America,s e-journal, ... multiple fields, including tectonics, oceanography, sedimentology, and paleontology, to ... to 6 million years ago; sedimentation in a piggyback ... Nevada; and a study of the South Balkan extensional ...
... Calif. For their landmark research leading to the ... flood-prone areas worldwide, Julia Bailey-Serres of UC ... David Mackill of the International Rice Research ... U.S. Department of Agriculture (USDA) with the 2008 USDA ...
Cached Biology News:Endangered sawfish focus of national collection and recovery efforts 2December Geosphere media highlights 2December Geosphere media highlights 3UC Riverside rice geneticist receives high honor from US Department of Agriculture 2UC Riverside rice geneticist receives high honor from US Department of Agriculture 3
... 6-Fam, Hex, Tet Cy3, Cy5, Texas ... Black Hole Quenchers Molecular Beacons* ... Joe 3 Dabcyl Dabcyl*Licensed from ... Double labelled probes are purified either by ...
... a high performance spectral scanning multimode ... With the advanced SkanIt Software it ... drug discovery assay development. Varioskan provides ... time-resolved fluorescence and photometric assays and ...
Roll of Mesh 5m x 25cm...
... Innovative HL-2000 HybriLinker combines the benefits of ... one self-contained, space saving unit. , ... Science Laboratory allows crosslinking ,and hybridization at one ... the HL-2000 operate independently of each other ...
Biology Products: