Navigation Links
SuperFect Transfection Reagent

osome, leading to pH inhibition of endosomal nucleases, which ensures stability of SuperFectDNA complexes.

Due to highly controlled chemical synthesis the activated-dendrimer molecules in SuperFect Reagent have a precise size and a defined shape. This ensures consistent transfection-complex formation and reproducible transfection results.

Activated-Dendrimer Structure

Schematic representation of an activated dendrimer (1). Note the highly branched structure.
SuperFectDNA Interaction
Model of the SuperFectDNA complex. SuperFect Reagent (purple balls) interacts with DNA (black) to form a ring-like (toroid-like) structure. The upper right section of the illustration shows naked DNA, the lower section shows the interaction between dendrimers and DNA inside the complex, and the upper left section shows the final complete coverage of DNA within the complex. Procedure

SuperFect Transfection Reagent is provided as a ready-to-use solution. The reagent is simply added to the DNA solution, mixed, and incubated for 510 minutes to allow SuperFectDNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. After a 23 hour incubation, a medium change is performed and the cells are incubated for expression of the transfected gene.

Applications

SuperFect Re
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Efficient Transfection of Neurospora Crassa
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. Efficient Transfection of Neurospora Crassa
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: SuperFect Transfection Reagent

(Date:7/27/2015)... ... 2015 , ... BlueInGreen® announced the addition of EnviroSales of ... world’s most efficient gas dissolution technology to all of Florida, excluding the panhandle. ... and municipal markets. With offices located in Sarasota, Sebring and Melbourne Beach, the ...
(Date:7/27/2015)... ... July 27, 2015 , ... Visual Assay was named as ... top 100 most innovative technologies and services of the past year. More than 70 ... Software/Services category. The winners will be announced at the 2015 R&D 100 Awards Dinner ...
(Date:7/24/2015)... NEW YORK , July 24, 2015 ... the following equities: PDL BioPharma Inc. (NASDAQ: PDLI ... Inc. (NASDAQ: STEM ), Northwest Biotherapeutics Inc. (NASDAQ: ... ACOR ). Free research report on PDL BioPharma can ... 23, 2015, the NASDAQ Composite ended at 5,146.41, down ...
(Date:7/24/2015)... 24, 2015 , Adds integrated ... Expands product offering for chronic kidney disease and oncology ... diabetes-related complications and dermatology , Increases group revenue ... or ,the Company"), a leading China ... marketing biopharmaceutical products, today announced that it has acquired ...
Breaking Biology Technology:BlueInGreen Brings Water Treatment Technology to Florida 2Visual Assay® Named Finalist in 53rd Annual R&D 100 Awards 2Biotechnology Stocks in Motion -- PDL BioPharma, Arena Pharma, StemCells, Northwest Biotherapeutics, and Acorda Therapeutics 2Biotechnology Stocks in Motion -- PDL BioPharma, Arena Pharma, StemCells, Northwest Biotherapeutics, and Acorda Therapeutics 3Biotechnology Stocks in Motion -- PDL BioPharma, Arena Pharma, StemCells, Northwest Biotherapeutics, and Acorda Therapeutics 4Biotechnology Stocks in Motion -- PDL BioPharma, Arena Pharma, StemCells, Northwest Biotherapeutics, and Acorda Therapeutics 53SBio Acquires Zhejiang Wansheng Pharmaceutical 23SBio Acquires Zhejiang Wansheng Pharmaceutical 33SBio Acquires Zhejiang Wansheng Pharmaceutical 4
... Sept. 14 Stemedica Cell Technologies, Inc., a world ... today that it has completed its proprietary master bank ... bank was manufactured in accordance with FDA Good Manufacturing ... the necessary safety testing required by the FDA and ...
... Sept. 13 Regenicin™, Inc. ... biotechnology company, announced today that John J. Weber, the ... Systems, USA, Inc. ("Fujifilm USA"), has agreed to join ... directors and interim Chief Financial Officer.  Weber brings over ...
... 13 SOCMA President & CEO Lawrence Sloan will lead ... coming Wednesday morning. The panel will address the "Shifting Landscape ... supply chain, reports Mark Alexay, the organizer. Sloan,s ... and SOCMA,s Bulk Drug Pharmaceutical Task Force. The BPTF was ...
Cached Biology Technology:Stemedica Announces Completion of Clinical Stem Cell Master Bank 2Former Fujifilm Medical Systems USA Executive John J. Weber Agrees to Join Regenicin™ as a Member of Its Board of Directors and Interim CFO 2
(Date:7/21/2015)... , July 21, 2015  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile ... wallet, announces that it has filed provisional patent ... Method. This invention highlights next ... authorizes an account, but also the user using ...
(Date:7/13/2015)... OXFORD, Conn. , Jul. 13, 2015 /PRNewswire/ ... the "Company"), a biometric authentication company focused on ... it has filed provisional patent 62/188684 for ... NXT-ID achieves another new convenient and secure method ... expands new, innovative payment methods, introduced with its ...
(Date:7/9/2015)... Calif. , July 9, 2015 /PRNewswire/ ... leading developer of human interface solutions, today ... the industry,s first fully hardware encapsulated fingerprint ... secure authentication technology is literally off the ... and biometric matching within the fingerprint sensor ...
Breaking Biology News(10 mins):NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3
... process used to roast coffee beans could give Britain,s ... some of the UKs leading energy crops by up ... by engineers from the University of Leeds, examined the ... when put through a mild thermal process called torrefaction ...
... Babies born preterm were more than twice as likely to ... new analysis of nearly 7 million U.S. live births published ... Health Journal. Preterm birth, live birth before 37 ... to the March of Dimes. More than a half million ...
... as neurons process information by joining into complex networks, ... But neurons dont just connect to other neurons, emphasizes ... they connect to very specific partners, at particular spots. ... Laboratory (CSHL), leads a team that has identified molecules ...
Cached Biology News:Analysis of millions of US births shows association between birth defects and preterm birth 2Scientists discover a molecular scaffold that guides connections between brain cells 2Scientists discover a molecular scaffold that guides connections between brain cells 3