Navigation Links
SuperFect Transfection Reagent

The proven activated dendrimer for transfection of a broad range of cell lines

Features and benefits
  • Fast procedure and easy handling
  • Suitable for a broad range of cell lines
  • Transfection in the presence of serum (see figure "Serum and DNA Quantity vs. Transfection Efficiency")
  • High transient and stable transfection efficiencies
  • Excellent reproducibility due to precise size and defined shape of activated dendrimers

Serum and DNA Quantity vs. Transfection Efficiency



Influence of serum and DNA quantity on transfection using SuperFect Reagent. 2 x 104 COS-7 cells were seeded per well in 96-well plates one day prior transfection. Cells were transfected using 0.12.0 g of a beta-galactosidasereporter plasmid and 3 l SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from four replicates assayed 48 h post-transfection.

Principle

SuperFect Reagent consists of activated-dendrimer molecules with a defined spherical architecture (1). Branches radiate from a central core and terminate at charged amino groups which can then interact with negatively charged phosphate groups of nucleic acids (see figure "Activated-Dendrimer Structure"). SuperFect Reagent assembles DNA into compact structures (see figure "SuperFectDNA Interaction") that bind to the cell surface and are taken into the cell by nonspecific endocytosis. The reagent buffers the pH of the end
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Efficient Transfection of Neurospora Crassa
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. Efficient Transfection of Neurospora Crassa
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: SuperFect Transfection Reagent

(Date:1/15/2014)... (PRWEB) January 15, 2014 This webinar ... nonclinical and clinical safety assessment in biosimilars. , Regulatory ... for biosimilar drug development, however the complex nature of ... quality, safety and efficacy extremely challenging. Based on the ...
(Date:1/15/2014)... More than 5 million Americans are currently living ... will die with Alzheimer’s or another dementia, according to ... many Americans into looking for ways to improve their ... cognitive disorders. Jonathan Weisman, president of Biohack Pure, is ...
(Date:1/15/2014)... Rochester, NY (PRWEB) January 15, 2014 The ... cause a major disease. One of these latent viruses is ... is rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... the theory, a study found that RA patients have high ...
(Date:1/15/2014)... Scottsdale, Arizona (PRWEB) January 15, 2014 ... growth for Scottsdale’s Brain State Technologies®. They saw continued ... University Medical Center who were awarded a $1 million ... papers published in “Brain and Behavior” a peer reviewed ...
Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3
... Australia, Dec. 17 Phosphagenics,Limited ("Phosphagenics") (ASX: POH, ... its Phase 1 clinical trial that showed its,delivery ... skin without causing disruption or irritation of any ... be the first company to,commercialize a sustained release ...
... MOUNTAINSIDE, N.J., Dec. 17 National Stem,Cell Holding, ... company,information, financial information, quotations and press releases are,posted ... Sheets., OTCIQ is a secure, web-based portal ... to facilitate communication with the,marketplace, promote transparency, mitigate ...
... Will Enhance Quality and Speed Image Transfer at Lower ... Cost While Ensuring 21 CFR Part 11 Compliance, ... network, today announced it has been selected by,Southwest Oncology Group ... groups in the United States, to provide a,secure, 21 CFR ...
Cached Biology Technology:Phosphagenics Announces Successful Phase 1 Transdermal Oxycodone Clinical Trial Results 2Phosphagenics Announces Successful Phase 1 Transdermal Oxycodone Clinical Trial Results 3National Stem Cell Holding, Inc. Posts Company and Financial Information on the OTCIQ and Has Retained New CFO and Counsel to File Registration Statement With SEC 2AG Mednet to Provide Image Network for Southwest Oncology Group 2
(Date:4/17/2014)... April 17, 2014The development of stem cell therapies to ... to characterize stem cell populations based on cell surface ... a new marker that is highly expressed in a ... blood, which they describe in an article in ... Ann Liebert, Inc., publishers. The article is available free ...
(Date:4/17/2014)... A new modeling study suggests that fish consumption ... infant exposure to long-lived contaminants like persistent organic ... a team of researchers including University of Toronto ... Wania, looks at how different levels of environmental ... behavior of chemicals in the body influenced exposure ...
(Date:4/17/2014)... anthrax detection cartridge developed at Sandia National Laboratories and ... easier, faster and cheaper. , Bacillus anthracis ... in soils all over the world and can cause ... animals. The bacteria can survive in harsh conditions for ... may occur through skin contact, inhalation of spores or ...
Breaking Biology News(10 mins):Fish consumption advisories fail to cover all types of contaminants 2Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3
... the only one of its kind in the world ... make-up of its closest endangered relatives. University of Manchester ... Leaf frogs to interbreed, producing the unique frog a ... mouth swab showed that the two parent frogs were actually ...
... have identified a single mutated gene that causes Hajdu-Cheney ... loss and osteoporosis (fragile bones). The study, published in ... possible causes of osteoporosis and highlights the gene as ... , There are only 50 reported cases of Hajdu-Cheney ...
... system developed in part by DOE,s Oak Ridge National ... the threats of insects, disease and wildfire. Led ... high-performance computing to incorporate remote sensing data from NASA ... identify abnormal vegetation patterns and the timing of seasonal ...
Cached Biology News:Unique frog helps amphibian conservation efforts 2Gene responsible for severe osteoporosis disorder discovered 2
Rabbit polyclonal to Oct2, prediluted ( Abpromise for all tested applications). entrezGeneID: 5452 SwissProtID: P09086...
Oct 2 Immunogen: Full length human protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
...
Phosphotyrosine IgG mouse monoclonal antibody: Alkaline Phosphatase conjugate...
Biology Products: