( ...)SuperFect Transfection Reagent

The proven activated dendrimer for transfection of a broad range of cell lines

Features and benefits

• Fast procedure and easy handling
  
• Suitable for a broad range of cell lines
  
• Transfection in the presence of serum (see figure "Serum and DNA Quantity vs. Transfection Efficiency")
  
• High transient and stable transfection efficiencies
  
• Excellent reproducibility due to precise size and defined shape of activated dendrimers

Serum and DNA Quantity vs. Transfection Efficiency



Influence of serum and DNA quantity on transfection using SuperFect Reagent. 2 x 104 COS-7 cells were seeded per well in 96-well plates one day prior transfection. Cells were transfected using 0.12.0 g of a beta-galactosidasereporter plasmid and 3 l SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from four replicates assayed 48 h post-transfection.

Principle

SuperFect Reagent consists of activated-dendrimer molecules with a defined spherical architecture (1). Branches radiate from a central core and terminate at charged amino groups which can then interact with negatively charged phosphate groups of nucleic acids (see figure "Activated-Dendrimer Structure"). SuperFect Reagent assembles DNA into compact structures (see figure "SuperFectDNA Interaction") that bind to the cell surface and are taken into the cell by nonspecific endocytosis. The reagent buffers the pH of the end
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