Navigation Links
SuperFect Transfection Reagent

The proven activated dendrimer for transfection of a broad range of cell lines

Features and benefits
  • Fast procedure and easy handling
  • Suitable for a broad range of cell lines
  • Transfection in the presence of serum (see figure "Serum and DNA Quantity vs. Transfection Efficiency")
  • High transient and stable transfection efficiencies
  • Excellent reproducibility due to precise size and defined shape of activated dendrimers

Serum and DNA Quantity vs. Transfection Efficiency



Influence of serum and DNA quantity on transfection using SuperFect Reagent. 2 x 104 COS-7 cells were seeded per well in 96-well plates one day prior transfection. Cells were transfected using 0.12.0 g of a beta-galactosidasereporter plasmid and 3 l SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from four replicates assayed 48 h post-transfection.

Principle

SuperFect Reagent consists of activated-dendrimer molecules with a defined spherical architecture (1). Branches radiate from a central core and terminate at charged amino groups which can then interact with negatively charged phosphate groups of nucleic acids (see figure "Activated-Dendrimer Structure"). SuperFect Reagent assembles DNA into compact structures (see figure "SuperFectDNA Interaction") that bind to the cell surface and are taken into the cell by nonspecific endocytosis. The reagent buffers the pH of the end
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
3. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
6. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
7. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
8. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
9. Efficient Transfection of Neurospora Crassa
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. Efficient Transfection of Neurospora Crassa
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: SuperFect Transfection Reagent

(Date:9/30/2014)... 30, 2014 Applying a well-known optical phenomenon ... Harvard University has demonstrated the ability to "paint" ... that holds promise for making future, flexible electronic ... sides of next-gen rocket ships and spacecraft with ... through oil in water, it becomes iridescent, revealing ...
(Date:9/30/2014)... Report Details Biosimilar drugs – ... Do you want to find sales potentials of ... for those biological drugs from 2014, helping you ... data, R&D trends, opportunities and selling prospects. In ... 2024 at overall world market, therapeutic class, molecule ...
(Date:9/30/2014)... Proove Biosciences , the commercial ... announce a new genetic test built upon research from ... Test will provide physicians with an objective way ... levels are stratified between individuals. With this new test, ... has a genetic predisposition that will affect their pain ...
(Date:9/30/2014)... Fast, semi-automatic creation of ... documents ChemAxon , a leader ... for life science research, launches ChemCurator, an application ... from documents. Markush structures are essential ... and understanding Markush structures is a difficult and ...
Breaking Biology Technology:Taking thin films to the extreme 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2
... Sept. 25 We are very proud to,announce ... Inc.,Outstanding customer service, product reliability, and a global,distribution ... company resulting,in a more powerful union of innovative ... product development. In 1980 Cotop International was ...
... 25 Medivation, Inc.,(Nasdaq: MDVN ) today announced ... the,inaugural companies in the new NASDAQ(R) NeuroInsights(R) Neurotech Index.,The ... return basis (Nasdaq:,NERV) and a total return basis (Nasdaq: ... 32 companies in the,neurotechnology industry is testament to our ...
... GeneGo, Inc., the leading,systems biology tools company, announced ... licensed access to all major GeneGo products. Using ... Squibb will have the ability to start with ... from both chemistry,and biology domains. Bristol-Myers Squibb can ...
Cached Biology Technology:Medivation Selected as Founding Member of New NASDAQ(R) NeuroInsights(R) Neurotech Index 2Medivation Selected as Founding Member of New NASDAQ(R) NeuroInsights(R) Neurotech Index 3GeneGo Enters Multi-Year Agreement With Bristol Myers Squibb for Access to GeneGo Software Suite and Databases 2
(Date:9/30/2014)... is published today in the first authoritative scientific study ... on both sides of the grouse moor management debate. ... the Ecohydrology of River basins) project has shown that ... support red grouse populations for gun sports, has significant ... properties, river water chemistry and river ecology. , A ...
(Date:9/30/2014)... all have in common? Unlike most eukaryotic multicellular ... diploid, these organisms are all polyploid, meaning they have ... have 3 and 4 sets of chromosomes, respectively, and ... in fact most plant species are polyploid. Polyploidy, or ... but only recently, with the development of molecular tools, ...
(Date:9/30/2014)... team of environmental scientists and engineers, led by ... a three-year, $5.25 million grant from the U.S. ... Research to make a direct positive impact on ... and disposal of radioactive contaminants. , "Understanding the ... dealing with environmental aspects of nuclear technologies is ...
Breaking Biology News(10 mins):Grouse moor burning causes widespread environmental changes 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Researchers get $5.25 million to advance nuclear technologies in South Carolina 2
... In the rain forests of the Congo, where mammals ... of buzzing insects blankets the atmosphere. Because it ... of the Congo,s insect biodiversity remains largely undiscovered. In ... Zootaxa , researchers at the University of Florida and ...
... of the department of dermatology at Boston University School of ... been named a 2012 Charter Fellow of the National Academy ... is among four faculty members at Boston University nominated to ... who are elected by their peers, represent 98 innovators from ...
... Due to the introduction of exotic pests and ... from forest ecosystems. In some cases, scientists can observe ... other cases, creative puzzle solving and analysis reveal unexpected ... the hemlock tree, University of Illinois landscape and ecosystem ...
Cached Biology News:Discovery of Africa moth species important for agriculture, controlling invasive plants 2Discovery of Africa moth species important for agriculture, controlling invasive plants 3Death of hemlock trees yields new life for hardwood trees, but at what cost to the ecosystem? 2Death of hemlock trees yields new life for hardwood trees, but at what cost to the ecosystem? 3
... exchange media are hydrophilic, ... binding capacity. The UNOsphere ... strong cation exchanger with ... 120 micrometers. Fully supported ...
... This CLS number is a new ... Cornings product number. If showing no ... old Sigma-Aldrich number (Z71,487-9) or contact ... clarifier: centre neck 100 mm, No. ...
Packaging: 6 bottles for 1 l ready-to-use-solution ID clarifier: ready-to-use solution ...
... S-X media are neutral, porous ... exclusion chromatography of lipophilic polymers ... organic eluents. The beads are ... carbon tetrachloride, dimethylformamide, ketones, aromatics, ...
Biology Products: