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Suggested applications for reverse transcriptases

Enzyme Full-Length cDNA High fidelity cDNA for cloning Amplification of viral RNA DDDRTa RACEa PTTa One-Step RT-PCR One-Step RT-PCR with carry-over prevention Expand Re-
verse Trans-
criptaseb +++ + ++ +++ ++ +++ ++ n.d. AMV Reverse Transcriptasec ++ + ++ + ++ +++ ++
(+++)d +
(-)d M-MuLV Reverse Transcriptase + + ++ ++ + ++ + n.d. C. therm Polymerase for RT in Two-Step RT-PCR + ++ +++ + ++ ++ +++ (+++)e +
(+)e Tth DNA Polymerase - - ++ + +/- +/- ++ + n.d., not determined a DDRT, differential display reverse transcription; RACE, rapid amplification of cDNA ends, PTT, Protein Truncation Test. b Not for sale in the U.S. c AMV Reverse Transcriptase is also included in the Titan One Tube RT-PCR System. d When used as a component of the Titan One Tube RT-PCR System. e When used as component of the C. therm. Polymerase One-Step RT-PCR System.


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