Navigation Links
Successful stabilization of gene expression profiles

rification. Whatever the storage temperature of the cells, intact RNA with high RIN values can be purified (Table 1).



Animal tissues
The traditional method of stabilizing RNA in animal tissues requires the use of liquid nitrogen or dry ice to freeze samples. A convenient alternative method is provided by RNAlater RNA Stabilization Reagent, which diffuses rapidly into samples at room temperature to immediately stabilize RNA. The reagent is also available in RNAlater TissueProtect Tubes for collection, stabilization, transport, and storage of tissues, and as part of RNeasy Protect Kits for stabilization and purification of RNA.


Bacteria
Bacteria often require vigorous methods for disruption and homogenization. During these processes, RNA degradation can occur. RNeasy Protect Bacteria Kits provide RNA stabilization while bacteria are still in culture. This allows sufficient time for disruption and homogenization during RNA purification with the same kits.


Human blood
A standardized method is essential for diagnostic laboratories that collect blood samples from multiple locations for RNA purification and analysis. This is provided by the PAXgene Blood RNA System, which is designed for in vitro diagnostic use and comprises PAXgene Blood RNA Tubes for blood collection and stabilization and the PAXgene Blood RNA Kit for RNA purification.


Visit www.qiagen.com/goto/QNews/RNA to find out more about QIAGENs complete range of solutions for RNA stabilization and purification



back t
'"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Keys to Successful Densitometry
2. Successful PCR amplification and subcloning of a GC-rich DNA fragment
3. General Considerations for Successful Transfection Experiments
4. Designing a Successful qRT-PCR Experiment
5. Precursor miRNAs for Successful miRNA Functional Studies
6. Recommendations for Successful siRNA Library Screens
7. Setting up Successful siRNA Library Screens
8. High-throughput gene expression analysis to screen for anti-asthma drugs
9. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Successful stabilization gene expression profiles

(Date:7/11/2014)... 2014 /CNW/ - Sunovion Pharmaceuticals Canada Inc. (Sunovion) today ... acetate) for use as a once-daily adjunctive therapy for ... who are not satisfactorily controlled with conventional therapy.  APTIOM ... under 18 years of age. Epilepsy ... according to Epilepsy Canada, it affects 0.6% of the ...
(Date:7/10/2014)... 2014 Product and process impurities ... abundance and are often “lost in the noise,” ... Join presenters Dr. Rowel Tobias, Senior Scientist, Protein ... Head of Quality at Nanotherapeutics, Inc., to lear ... speed detection and quantitation while achieving the required ...
(Date:7/10/2014)... 2014 Unraveling life’s mysteries can intrigue, ... Archer Family purchased a DNA test to ... up with a lifetime of memories, sharing, and closeness. ... members’ DNA genuinely brought the family closer together. ... into genetic history and prior to taking the ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Senior supply ... and professional associations, addressed the challenges of “Reducing Cost, ... Device Industries” through strategic sourcing. Describing the partnership of ... the Supply Chain Management Institute ( SCMI) of ... on June 24 at the Kroc Institute of Peace ...
Breaking Biology Technology:Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 2Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 3Sunovion Pharmaceuticals Canada Inc. Announces Health Canada Approval of APTIOM(TM) (eslicarbazepine acetate) as a Once-Daily Adjunctive Treatment for Partial-Onset Seizures in Patients with Epilepsy 4Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4
... sunlight at a modest cost is a challenge with ... paper in a special energy issue of Optics ... a relatively new approach to solar cells: lacing them ... the article, this approach has the potential to greatly ...
... GREENBELT, Md. - Engineers at the University of Idaho ... in upcoming NASA missions that will study the Earth ... of a grant technical officer Pen-Shu Yeh, a senior ... the engineering team at the University of ...
... Md. - Engineers at the University of Idaho have ... upcoming NASA missions that will study the Earth and ... a grant technical officer Pen-Shu Yeh, a senior engineer ... engineering team at the University of Idaho,s ...
Cached Biology Technology:Enhancing solar cells with nanoparticles 2New one-of-a-kind technology will fly on 2 NASA missions 2New one-of-a-kind technology will fly on 2 NASA missions 3New one-of-a-kind technology will fly on two NASA missions 2New one-of-a-kind technology will fly on two NASA missions 3
(Date:7/11/2014)... Researchers from Salk Institute for Biological Studies, BGI, ... the safety and reliability of the existing targeted ... method, TALEN-HDAdV, which could significantly increased gene-correction efficiency ... study published online in Cell Stell Cell ... cell-based gene therapy. , The combination of stem ...
(Date:7/11/2014)... July 11, 2014 Researchers have pioneered a ... The new technology, called Virtual Finger, allows scientists ... like neurons and synapses using the flat surface ... makes 3D imaging studies orders of magnitude more ... unprecedented level across many areas of experimental biology. ...
(Date:7/11/2014)... is evidence that under the normal circumstances, ... development, maintain neuronal environment, and exhibit therapeutic ... neurodegenerative disease. Previous studies have found that ... after chemical induction have reduced viability, which ... Prof. Xiaodong Yuan, Kailuan General Hospital, Hebei ...
Breaking Biology News(10 mins):A new genome editing method brings the possibility of gene therapies closer to reality 2Virtual finger enables scientists to navigate and analyze complex 3D images 2
... varieties with higher yields and enhanced nutritional content, researchers ... with scientists at nine other institutions in an attempt ... endeavor, led by researchers at the U.K.-based Biotechnology and ... in the journal Nature . "This work ...
... release is available in Spanish . WASHINGTON, ... as part of an international team have completed a "shotgun sequencing" ... Nature reported today. The achievement is expected to increase ... wheat varieties with enhanced nutritional value. "By unlocking the ...
... code of one of the world,s most important crops. The ... genome, published today in Nature , is a major ... better able to cope with disease, drought and other stresses ... wheat genes, and insights into the links between them, lays ...
Cached Biology News:UC Davis researchers aid effort to sequence the complex wheat genome 2USDA scientists and cooperators sequence the wheat genome in breakthrough for global food security 2USDA scientists and cooperators sequence the wheat genome in breakthrough for global food security 3Major breakthrough in deciphering bread wheat's genetic code 2Major breakthrough in deciphering bread wheat's genetic code 3Major breakthrough in deciphering bread wheat's genetic code 4
... The VSOS-4 Orbital Shaker is designed to ... provide a uniform motion that is both mixing ... VSOS-4 Orbital Shaker provides a smooth, quiet, reproducible ... a wide range of laboratory applications such as ...
... Titer Kit is designed for determination of baculovirus ... days as in other methods. Classical determination of ... plaques, but the FastPlax Kit takes advantage of ... glycoprotein on the cell surface as early as ...
... state-of-the-art software for all your biopharmaceutical applications ... a single, scalable software package. The graphical ... the control of all Freedom instruments from ... 100 with one liquid handling arm up ...
... Kit is an advanced system for isolating DNA from ... be used directly for any downstream application. ... to use in 56 minutes Low melting point ... and TBE gels No specialised equipment required ...
Biology Products: