Navigation Links
Successful stabilization of gene expression profiles

rification. Whatever the storage temperature of the cells, intact RNA with high RIN values can be purified (Table 1).



Animal tissues
The traditional method of stabilizing RNA in animal tissues requires the use of liquid nitrogen or dry ice to freeze samples. A convenient alternative method is provided by RNAlater RNA Stabilization Reagent, which diffuses rapidly into samples at room temperature to immediately stabilize RNA. The reagent is also available in RNAlater TissueProtect Tubes for collection, stabilization, transport, and storage of tissues, and as part of RNeasy Protect Kits for stabilization and purification of RNA.


Bacteria
Bacteria often require vigorous methods for disruption and homogenization. During these processes, RNA degradation can occur. RNeasy Protect Bacteria Kits provide RNA stabilization while bacteria are still in culture. This allows sufficient time for disruption and homogenization during RNA purification with the same kits.


Human blood
A standardized method is essential for diagnostic laboratories that collect blood samples from multiple locations for RNA purification and analysis. This is provided by the PAXgene Blood RNA System, which is designed for in vitro diagnostic use and comprises PAXgene Blood RNA Tubes for blood collection and stabilization and the PAXgene Blood RNA Kit for RNA purification.


Visit www.qiagen.com/goto/QNews/RNA to find out more about QIAGENs complete range of solutions for RNA stabilization and purification



back t
'"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Keys to Successful Densitometry
2. Successful PCR amplification and subcloning of a GC-rich DNA fragment
3. General Considerations for Successful Transfection Experiments
4. Designing a Successful qRT-PCR Experiment
5. Precursor miRNAs for Successful miRNA Functional Studies
6. Recommendations for Successful siRNA Library Screens
7. Setting up Successful siRNA Library Screens
8. High-throughput gene expression analysis to screen for anti-asthma drugs
9. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Successful stabilization gene expression profiles

(Date:10/22/2014)... The Americas Inorganic Refrigerants Market report defines and ... analysis and forecast of revenue. , Browse through ... report, to get an idea of the in-depth ... the segmentation in the Americas inorganic refrigerants market, ... , http://www.micromarketmonitor.com/market/americas-inorganic-refrigerants-4191654241.html , Inorganic refrigerants ...
(Date:10/22/2014)... 22, 2014 Currently offering a ... & D Weighing, Rice Lake, and Ohaus, Pipette.com ... . The Sartorius CPA Semi-Micro Balance is ideal ... precise, and user-friendly laboratory balance. , Sartorius is ... their laboratory balances are well known for their ...
(Date:10/22/2014)... Oct. 22, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" or ... next steps for its Oral Amphotericin B program.  ... in vitro work involving samples from ... plans to complete pre clinical studies and regulatory ... 1A clinical trial, utilizing approximately $700,000 of funding ...
(Date:10/20/2014)... Oct. 20, 2014 Mapp Biopharmaceutical,s valiant ... antibody therapeutic to fight the Ebola outbreak will ... time-consuming the production of pharmaceuticals can be, according ... said that while some may be taken aback ... those with industry knowledge are well aware of ...
Breaking Biology Technology:The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 2The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 3Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 3iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3
... Calif., Aug. 6 Telik, Inc. (Nasdaq: TELK ) today ... the second quarter ended June 30, 2009, compared with a net loss ... 2008. , , For the quarter ended June 30, ... million in the 2008 second quarter. Operating expenses in the 2009 ...
... , , EMERYVILLE, Calif., ... firm, added John E. Sealander, AIA, ACHA, NCARB, LEED(R) AP, ... Ratcliff ( http://www.ratcliffarch.com ) provides a ... and civic clients throughout the Western United States. , ...
... , , LOS ... $6 million to an 82 year-old Chinese female immigrant who suffered ... alleged that the Defendant, an osteopathic physician and registered pharmacist, prescribed ... alleged that she did not have gout. The doctor claimed ...
Cached Biology Technology:Telik Announces Second Quarter 2009 Financial Results 2Telik Announces Second Quarter 2009 Financial Results 3Telik Announces Second Quarter 2009 Financial Results 4Ratcliff Adds Architect John Sealander to Healthcare Practice Group 2The Law Offices of Brian D. Witzer Wins 6+ Million Dollar Verdict 2
(Date:10/22/2014)... BEDFORD, Mass., Oct. 21, 2014 Aware, Inc. (NASDAQ: ... and services, today reported financial results for its third quarter ... quarter of 2014 was $6.0 million, an increase of 40% ... Operating income in the third quarter of 2014 was $4.1 ... 2013. Higher operating income in the current three month period ...
(Date:10/17/2014)... Accurate knowledge regarding Ebola is critical and pertinent for ... hazardous global outbreak and epidemic. The Journal, Disaster ... special issue, Ebola Virus and Public Health, to surround ... in this critical societal moment. , On October ... for Clinicians . The primer was prepared by Dr. ...
(Date:10/16/2014)... Institute of Marine Science have received a 3-year, ... identify the streams and wetlands most vulnerable to ... local governments and citizens conserve these important ecosystems. ... of tidal-marsh observations first gathered by VIMS scientists ... can today,s researchers accurately map the slow but ...
Breaking Biology News(10 mins):Aware, Inc. Reports Third Quarter 2014 Financial Results 2Aware, Inc. Reports Third Quarter 2014 Financial Results 3Aware, Inc. Reports Third Quarter 2014 Financial Results 4Aware, Inc. Reports Third Quarter 2014 Financial Results 5Aware, Inc. Reports Third Quarter 2014 Financial Results 6Aware, Inc. Reports Third Quarter 2014 Financial Results 7EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3
... Ore. A new study finds that utilities aren,t ... are needed to make energy efficiency as attractive as ... issue of Environmental Law , examines key differences ... Scott, an assistant professor at Oregon State University, outlines ...
... personal, portable, and wearable air pollution sensor, developed under ... at the Health Datapalooza in Washington, D.C. The grand ... The challenge was held by the National Institutes ... Health Information Technology (ONC) of the U.S. Department of ...
... associations between cognitive ability and cortical grey matter in old ... childhood. The joint study by the Montreal Neurological Institute ... Edinburgh, UK was published today, June 4 in Molecular ... preserving brain cortical thickness was a determining factor in superior ...
Cached Biology News:Study finds disincentives to energy efficiency can be fixed 2NIH, ONC, and EPA award $100,000 to winner of health and technology challenge 2New study explains cognitive ability differences among the elderly 2