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Successful stabilization of gene expression profiles


RNA in a biological sample becomes extremely unstable upon collection of the sample, leading to changes in the gene expression profile. For accurate gene expression analysis in applications such as real-time RT-PCR and microarray analysis, it is important to stabilize the RNA immediately. QIAGEN provides products which enable stabilization and purification of high-quality RNA from animal tissues, bacteria, and human blood and now from human saliva and sorted or cultured cells. All products deliver immediate RNA stabilization at ambient temperature, eliminating the need for liquid nitrogen or dry ice.


Human saliva
RNA in saliva is already degrading prior to collection, making RNA stabilization critical. The RNeasy Protect Saliva Mini Kit provides immediate RNA stabilization upon collection of saliva followed by RNA purification (Figure 1). Stabilized saliva samples can be stored or transported at room temperature for up to 14 days prior to RNA purification or can be archived at 20C or 80C.


Sorted or cultured cells
In experiments where cells are harvested and stored for RNA purification and analysis at a later date, RNA stabilization is typically achieved by pelleting and freezing cells. If the cells need to be transported, dry ice is required to keep the cells frozen. The RNeasy Protect Cell Mini Kit provides a more convenient solution for RNA stabilization and purification. Supplied with the kit is RNAprotect Cell Reagent, which immediately stabilizes RNA in cells at room temperature without the need to remove cell-culture medium (Figure 2). The stabilized cells can then be transported at ambient temperature or stored frozen prior to RNA pu rification. Whatever the storage temperature of the cells, intact RNA with high RIN values can be purified (Table 1).



Animal tissues
The traditional method of stabilizing RNA in animal tissues requires the use of liquid nitrogen or dry ice to freeze samples. A convenient alternative method is provided by RNAlater RNA Stabilization Reagent, which diffuses rapidly into samples at room temperature to immediately stabilize RNA. The reagent is also available in RNAlater TissueProtect Tubes for collection, stabilization, transport, and storage of tissues, and as part of RNeasy Protect Kits for stabilization and purification of RNA.


Bacteria
Bacteria often require vigorous methods for disruption and homogenization. During these processes, RNA degradation can occur. RNeasy Protect Bacteria Kits provide RNA stabilization while bacteria are still in culture. This allows sufficient time for disruption and homogenization during RNA purification with the same kits.


Human blood
A standardized method is essential for diagnostic laboratories that collect blood samples from multiple locations for RNA purification and analysis. This is provided by the PAXgene Blood RNA System, which is designed for in vitro diagnostic use and comprises PAXgene Blood RNA Tubes for blood collection and stabilization and the PAXgene Blood RNA Kit for RNA purification.


Visit www.qiagen.com/goto/QNews/RNA to find out more about QIAGENs complete range of solutions for RNA stabilization and purification



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