Navigation Links
Successful stabilization of gene expression profiles


RNA in a biological sample becomes extremely unstable upon collection of the sample, leading to changes in the gene expression profile. For accurate gene expression analysis in applications such as real-time RT-PCR and microarray analysis, it is important to stabilize the RNA immediately. QIAGEN provides products which enable stabilization and purification of high-quality RNA from animal tissues, bacteria, and human blood and now from human saliva and sorted or cultured cells. All products deliver immediate RNA stabilization at ambient temperature, eliminating the need for liquid nitrogen or dry ice.


Human saliva
RNA in saliva is already degrading prior to collection, making RNA stabilization critical. The RNeasy Protect Saliva Mini Kit provides immediate RNA stabilization upon collection of saliva followed by RNA purification (Figure 1). Stabilized saliva samples can be stored or transported at room temperature for up to 14 days prior to RNA purification or can be archived at 20C or 80C.


Sorted or cultured cells
In experiments where cells are harvested and stored for RNA purification and analysis at a later date, RNA stabilization is typically achieved by pelleting and freezing cells. If the cells need to be transported, dry ice is required to keep the cells frozen. The RNeasy Protect Cell Mini Kit provides a more convenient solution for RNA stabilization and purification. Supplied with the kit is RNAprotect Cell Reagent, which immediately stabilizes RNA in cells at room temperature without the need to remove cell-culture medium (Figure 2). The stabilized cells can then be transported at ambient temperature or stored frozen prior to RNA pu rification. Whatever the storage temperature of the cells, intact RNA with high RIN values can be purified (Table 1).



Animal tissues
The traditional method of stabilizing RNA in animal tissues requires the use of liquid nitrogen or dry ice to freeze samples. A convenient alternative method is provided by RNAlater RNA Stabilization Reagent, which diffuses rapidly into samples at room temperature to immediately stabilize RNA. The reagent is also available in RNAlater TissueProtect Tubes for collection, stabilization, transport, and storage of tissues, and as part of RNeasy Protect Kits for stabilization and purification of RNA.


Bacteria
Bacteria often require vigorous methods for disruption and homogenization. During these processes, RNA degradation can occur. RNeasy Protect Bacteria Kits provide RNA stabilization while bacteria are still in culture. This allows sufficient time for disruption and homogenization during RNA purification with the same kits.


Human blood
A standardized method is essential for diagnostic laboratories that collect blood samples from multiple locations for RNA purification and analysis. This is provided by the PAXgene Blood RNA System, which is designed for in vitro diagnostic use and comprises PAXgene Blood RNA Tubes for blood collection and stabilization and the PAXgene Blood RNA Kit for RNA purification.


Visit www.qiagen.com/goto/QNews/RNA to find out more about QIAGENs complete range of solutions for RNA stabilization and purification



back t o top


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Keys to Successful Densitometry
2. Successful PCR amplification and subcloning of a GC-rich DNA fragment
3. General Considerations for Successful Transfection Experiments
4. Designing a Successful qRT-PCR Experiment
5. Precursor miRNAs for Successful miRNA Functional Studies
6. Recommendations for Successful siRNA Library Screens
7. Setting up Successful siRNA Library Screens
8. High-throughput gene expression analysis to screen for anti-asthma drugs
9. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... CAMBRIDGE, Mass. , June 23, 2016 /PRNewswire/ ... the development of novel compounds designed to target ... compound, napabucasin, has been granted Orphan Drug Designation ... in the treatment of gastric cancer, including gastroesophageal ... cancer stemness inhibitor designed to inhibit cancer stemness ...
(Date:6/23/2016)... Prostate Cancer Foundation (PCF) is pleased to announce 24 new Young Investigator ... Members of the Class of 2016 were selected from a pool of 128 ... About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... In a ... Hospital in Denmark detail how a patient who developed lymphedema after being treated for ... The results could change the paradigm for dealing with this debilitating, frequent side effect ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is exhibiting at the Pennsylvania Convention Center and will showcase its product’s latest ... ClinCapture will also be presenting a scientific poster on Disrupting Clinical Trials in ...
Breaking Biology Technology:
(Date:3/31/2016)... March 31, 2016   ... or the "Company") LegacyXChange is excited to ... its soon to be launched online site for trading ... ) will also provide potential shareholders a sense ... technology to an industry that is notorious for fraud. ...
(Date:3/29/2016)... LegacyXChange, Inc. (OTC: LEGX ... Protect are pleased to announce our successful effort to ... of writing instruments, ensuring athletes signatures against counterfeiting and ... athletes on LegacyXChange will be assured of ongoing proof ... Bill Bollander , CEO states, "By inserting ...
(Date:3/22/2016)... March 22, 2016 ... Sensors Market for Consumer Industry by Type (Image, ... Application (Communication & IT, Entertainment, Home Appliances, ... Forecast to 2022", published by MarketsandMarkets, the ... to reach USD 26.76 Billion by 2022, ...
Breaking Biology News(10 mins):