Navigation Links
Successful stabilization of gene expression profiles


RNA in a biological sample becomes extremely unstable upon collection of the sample, leading to changes in the gene expression profile. For accurate gene expression analysis in applications such as real-time RT-PCR and microarray analysis, it is important to stabilize the RNA immediately. QIAGEN provides products which enable stabilization and purification of high-quality RNA from animal tissues, bacteria, and human blood and now from human saliva and sorted or cultured cells. All products deliver immediate RNA stabilization at ambient temperature, eliminating the need for liquid nitrogen or dry ice.


Human saliva
RNA in saliva is already degrading prior to collection, making RNA stabilization critical. The RNeasy Protect Saliva Mini Kit provides immediate RNA stabilization upon collection of saliva followed by RNA purification (Figure 1). Stabilized saliva samples can be stored or transported at room temperature for up to 14 days prior to RNA purification or can be archived at 20C or 80C.


Sorted or cultured cells
In experiments where cells are harvested and stored for RNA purification and analysis at a later date, RNA stabilization is typically achieved by pelleting and freezing cells. If the cells need to be transported, dry ice is required to keep the cells frozen. The RNeasy Protect Cell Mini Kit provides a more convenient solution for RNA stabilization and purification. Supplied with the kit is RNAprotect Cell Reagent, which immediately stabilizes RNA in cells at room temperature without the need to remove cell-culture medium (Figure 2). The stabilized cells can then be transported at ambient temperature or stored frozen prior to RNA pu
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Keys to Successful Densitometry
2. Successful PCR amplification and subcloning of a GC-rich DNA fragment
3. General Considerations for Successful Transfection Experiments
4. Designing a Successful qRT-PCR Experiment
5. Precursor miRNAs for Successful miRNA Functional Studies
6. Recommendations for Successful siRNA Library Screens
7. Setting up Successful siRNA Library Screens
8. High-throughput gene expression analysis to screen for anti-asthma drugs
9. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Successful stabilization gene expression profiles

(Date:12/22/2014)... The American Journal of Hematology/Oncology, ... reviews and editorials addressing developments and pathways related ... a provocative article exploring the role of follicle-stimulating ... treatment of prostate cancer. , The article ... possibility that there could be a connection to ...
(Date:12/19/2014)... December 19, 2014 Bioject Medical ... and manufacturer of needle-free injection technology, today announced ... Immunomic Therapeutics, Inc. (“ITI”) for ITI to use ... its LAMP™ vaccine platform. , In the ... Worldwide license to the Biojector®-2000 that triggers based ...
(Date:12/19/2014)... Ill. , Dec. 19, 2014 Naurex ... develop novel drugs for diseases of the central nervous ... president and chief executive officer, will present at the ... Riedel,s presentation will take place at 3:00 p.m. PST ... Francis in San Francisco, Calif. ...
(Date:12/19/2014)... , Dec. 19, 2014 Decision Resources ... will grow at a 12 percent compound annual growth ... of the aging population and the increasing adoption of ... America will also spur demand for dental ... dental implant and periodontal treatments. Other key ...
Breaking Biology Technology:Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 2Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 3Prostate Cancer Experts Dissect the Role of Follicle-stimulating Hormone in the Development, Progression and Potential Treatment of Prostate Cancer 4Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 2Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 3Naurex to Present at 33rd Annual J.P. Morgan Healthcare Conference 2Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 2Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 3
... ROTTERDAM, Netherlands, April 24 LyondellBasell Industries today ... voluntarily added to Lyondell Chemical Company,s reorganization filing ... to protect the European holding company against claims ... LyondellBasell Industries AF S.C.A. is a holding company ...
... Maryland have published their assembly of the domestic cow ... the genetics community. The new version of the cow ... both completeness and accuracy. The article describing their research ... Genome Biology . , The research team led ...
... (Nasdaq: KERX ) today announced the appointment of ... Executive Officer, replacing Michael S. Weiss, effective immediately. Mr. ... since September 2007. Mr. Tarnok spent the majority of ... as Finance Director -- US Manufacturing. From 2000-2007, Mr. ...
Cached Biology Technology:LyondellBasell Adds Holding Company to Chapter 11 Protection 2New cow genome sequence released 2Keryx Biopharmaceuticals, Inc. Announces Michael P. Tarnok as Interim Chairman and Chief Executive Officer 2
(Date:12/3/2014)... , Dec. 2, 2014   Marvin Test ... innovative test solutions for military, aerospace, and manufacturing ... of its successful TS-900 PXI semiconductor test ... features of high-end systems to customers at a ... compared to traditional ATE. "Our ...
(Date:11/21/2014)... , November 18, 2014 ... Systems Market by Systems (Video, RFID, Access Control, Intrusion ... Factories, Hotels, Banks, Government), Component Service Geography - Global ... Systems Market is projected to be around $25 Billion ... by 2020, growing at a CAGR of 8.69%. ...
(Date:11/21/2014)... MOUNTAIN VIEW, Calif. , Nov. 20, 2014 /PRNewswire/ ... for higher driver efficiency are piloting the North American ... the number of accidents growing, gesture recognition systems that ... will make a mark in the industry. ... of the Automotive Gesture Recognition Market in ...
Breaking Biology News(10 mins):Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... N.J., Aug. 14 SyntheMed, Inc. (OTC,Bulletin Board: SYMD), ... a private placement in which it received $2.8 million ... CEO, stated, "The,proceeds of this financing will help fund ... fourth quarter of this year." At the closing, ...
... Investors Convert Debentures to Equity, JERICHO, N.Y., Aug. ... developer and supplier of consumer,biometric products, today announced that ... Series B common stock warrants that such holders,must surrender ... The Company notified holders of 375,000 series B ...
... in September issue of Nature Methods Shows Application of ... Attachment ... Expression., GENEVA, Switzerland, Aug. 21 Researchers at ... identification of novel,high-performance Genetic Elements also known as matrix ...
Cached Biology News:SyntheMed Completes $2.8 Million Equity Financing 2bioMETRX, Inc. Calls Series B Warrants 2Selexis Announces Advanced Approach to Maximize Power of Genetic Elements for Rapid Development of High Performance Cell Lines 2
Macrophage-stimulating protein receptor...
Anti-MMP-13 Collagenase-3, amino terminal end of active enzyme; rabbit host...
Mouse monoclonal [SPM302] to BCAR1, prediluted ( Abpromise for all tested applications). entrezGeneID: 9564 SwissProtID: P56945...
Phosphotyrosine IgG mouse monoclonal antibody: Alkaline Phosphatase conjugate...
Biology Products: