Navigation Links
Sub-Picomolar Fluorescein

Introduction
The SPEX FLUOROLOG spectrofluorometer is capable of detecting sub-picomolar ( < 1012 M) fluorescein according to the conventions established by the ASTM Committee E-13 on Molecular Spectroscopy (ANSI/ASTM E579-76). Other commercial manufacturers of spectrofluorometers specify their Minimum Detectable Concentration (MDC) somewhat differently. This technical note demonstrates how we achieve such a low MDC.

Experiment
The measurements were taken on a ing mode FLUOROLOG spectrofluorometer with a 450-W (521 nm) xenon lamp, and a cooled R928 photomultiplier operated at 900 V in the photon-counting mode. The bandpass was set to 4.0 nm on both excitation and emission spectrometers. Integration time was 11020 s, with a single scan and no smoothing. Excita-490 542.5 595 tion of the sample was at 480 nm. The acquisition Wavelength (nm)mode was S1/R1, that is, the emission signal was Figure 2. Raman spectrum of water. compensated by a reference quantum counter with voltage adjusted for a reading of 1.0 A. The scans At the highest wavelengths, at the extreme right were taken under ambient room conditions.

Results and Discussion
Figure 1 shows spectra of 400 fM (0.4 pM or 4 1013 M) fluorescein in 0.01-N NaOH, a blank of solely 0.01-N NaOH, and the subtracted spectrumof fluorescein without the solvent. The large peak near 520 nm clearly shows the presence of fluorescein at a 0.4-pM concentration.

At the highest wavelengths, at the extreme right of Figure 1, you can see the edge of the water Raman O-H stretch. A clearer graph of this region is shown in Figure 2, under 480-nm excitation. The Raman peak is inherently broad, centered at 575 nm, along with a weaker H-O-H bending mode near 521 nm. The bending mode is also visible in the solvent-containing spectra in Figure 1. This weak bending mode demonstrates the excellent sensitivity of the FLUOROLOG. In Figures 1 and 3, spectr
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Setting up the laboratory to avoid contamination
2. Reduce Errors and Save Time When Setting Up PCRs
3. Setting up Successful siRNA Library Screens
4. LiquiChip Kits for bead-based cytokine and kinase assays
5. Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
6. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sub Picomolar Fluorescein

(Date:5/26/2015)... 26, 2015 Global Stem Cells ... plans for the company’s first cellular and regenerative medicine ... a full day, hands-on stem cell training class Oct. ... Garcia Gimenez, M.D., President of Therapeutic Confrontations (CONFTERA) in ... increasing interest in regenerative medicine and recent advances in ...
(Date:5/26/2015)... Texas , May 26, 2015  A simple ... Health Science Center to detect early Alzheimer,s disease is ... adults. Detailed standardized guidelines that are needed ... have been published in Alzheimer,s & Dementia . ... of an international working group that includes UNT Health ...
(Date:5/26/2015)... mája 2015 Kevin Xu , ... prezidentom Barackovi Obamovi a Billovi Clintonovi za ich ... Dr. Rongxiang Xu , otca Kevina. ... Dr. Xu bol pionier, inovátor, humanitárny pracovník ... v oblasti vedy a príkladné vedenie svojej komunity ...
(Date:5/26/2015)... 26, 2015 Oramed Pharmaceuticals Inc. ... pharmaceutical company focused on the development of oral drug ... study protocol for the company,s Phase IIb trial of ... and Drug Administration (FDA). The submission was made under ... The Phase IIb study of ORMD-0801 for type ...
Breaking Biology Technology:Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 3Guidelines bring UNT Health Science Center's blood test for Alzheimer's one step closer 2Dr. Rongxiang Xu, O legende 2Oramed Submits Protocol to U.S. FDA for Its Phase IIb Oral Insulin Study 2Oramed Submits Protocol to U.S. FDA for Its Phase IIb Oral Insulin Study 3
... a Magellan,Biosciences company and leader in novel HPLC ... QA/QC, life science, and,analytical-chemistry customers worldwide, announced that ... American Technology Innovation Award in the,field of integrated ... to ESA,s Vice President of HPLC Products and ...
... US Oncology Specialty, LP, the,state-of-the-art pharmaceutical distribution ... Achievement Award from the National,Safety Council for its ... to companies who produce the "best of the ... organizations must report,days away from work resulting from ...
... Corporation (Nasdaq: UTHR ) and its wholly-owned subsidiary,Lung ... Drug Administration,(FDA) has accepted for review the New Drug ... arterial hypertension. The NDA,will be subject to a standard ... deadline of April 30, 2009., United Therapeutics Corporation ...
Cached Biology Technology:ESA Receives 2008 Frost & Sullivan North American Technology Innovation Award for Development of Integrated Metabolomics Systems 2ESA Receives 2008 Frost & Sullivan North American Technology Innovation Award for Development of Integrated Metabolomics Systems 3US Oncology Pharmaceutical Distribution Service Wins National Safety Council Award 2
(Date:4/27/2015)... ATLANTA , April 27, 2015  For more ... and trade show has been the premier worldwide event ... Systems 2015 is a must-attend event for any local, ... insight into the emerging commercial markets and current applications ... and innovations of this projected $48 billion industry, and ...
(Date:4/20/2015)... , April 20, 2015 Huntington Memorial ... Valley to implant a new miniaturized, wireless monitoring sensor ... is the first and only FDA-approved heart failure monitoring ... admissions when used by physicians to manage heart failure. ... that is implanted in the pulmonary artery (PA) during ...
(Date:4/14/2015)... April 14, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... the 2015 New York Design Awards under the category  ... York City Design Awards are part of a global ... 2,500 ratings from the marketplace, industry and judging panel. ...
Breaking Biology News(10 mins):UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
... A team composed of Harvard students and alumni ... Africa 2008 Development Marketplace competition, held in Accra, Ghana ... fuel cell-based lighting systems suitable for Sub-Saharan Africa, netted ... World Bank, as only 26 percent of Africas population ...
... ARBOR, Mich.---The mouse is a stalwart stand-in for humans ... percent identical. But identical genes may behave differently in ... evolutionary biologists Ben-Yang Liao and Jianzhi Zhang reveals. ... mouse models in studying human disease, appear in the ...
... Japanese researchers have found a strong link between tooth ... head and neck, and lung. They suggest that preservation ... In the May issue of Cancer Epidemiology, Biomarkers ... Cancer Research, scientists from Aichi Cancer Center in Nagoya ...
Cached Biology News:Undergraduates develop 'dirt-powered' microbial fuel cells to light Africa 2Undergraduates develop 'dirt-powered' microbial fuel cells to light Africa 3Mouse can do without man's most treasured genes 2Mouse can do without man's most treasured genes 3Tooth loss strongly linked to risk of esophageal, head and neck, and lung cancer 2
Barcoded Slides, 5 Packs of 5 Slides (25/Case)...
Adult Bovine Serum US Origin...
Anti-Mouse Qa.m8, Ascites (Clone 5035-24.1) (mouse IgG3)...
... 13(S)-HODE-d4 contains 4 deuterium atoms at the 9, ... for use as an internal standard for the ... 13(S)-HODE is produced by incubation of linoleic acid ... shown to inhibit the adhesion of tumor cells ...
Biology Products: