Navigation Links
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

) and certain cell types (Chlamydomonas, yeast, and maize callus) occurs using sub-micron sized gold particles. Smaller particles are probably more efficient than larger particles for Biolistic transformation in these systems because they cause less cell damage, resulting in better survival. Organelles could be irreversibly destroyed upon impact with large particles. In the case of maize callus, while cells bombarded with both 0.6 and 1.0 gold particles transiently express equivalent levels of anthocyanin four days postbombardment, cells bombarded with the smaller particles gave rise to many more stable transformants, most likely because they sustained less cell damage which could be more easily repaired.

Advantages of gold microparticles include their uniform size and shape and their biological inertness. In contrast, tungsten microparticles are irregular in shape and heterogeneous in size, can be toxic to cells, are subject to surface oxidation that can alter DNA binding, and, over time, can catalytically degrade DNA bound to them (Sanford, et al., 1993). Thus, because of higher transformation efficiency of gold particles, greater variability in results using tungsten, and the need for special precautions in particle preparation, handling, and transformation procedures when using tungsten, gold particles are superior to tungsten particles for Biolistic applications. 0.6 gold microparticles can be used with the Yeast Optimization Kit (catalog number 170-3100) to introduce the novice to the principles of particle bombardment.

The size of small particles may be determined by several methods, including molecular sieving, sedimentation, light scattering, electrical sensing, optical sensing, and
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Superior Gene Silencing Using Adenoviral Vectors
2. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Efficient Transfection of Neurospora Crassa
10. Efficient Transfection of Neurospora Crassa
11. Efficient Recovery of Ultrapure Plasmid DNA
Post Your Comments:
(Date:1/14/2014)... Carahsoft and CDS Federal Services have scheduled ... 2pm EST (11am PST), “Natural Language Processing: Converting Raw ... technology can turn raw, heterogeneous data into actionable knowledge ... online webinar will last approximately one hour. , Synopsis: ...
(Date:1/14/2014)... The largest international professional organization of scientists ... derivatives thereof has endorsed an educational program that ... challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural Product ...
(Date:1/14/2014)... 2014 Global Record Systems, LLC, ... technology solutions for patients, physicians, the biopharmaceutical industry, ... today the signing of a three-year Research Collaboration ... Administration (FDA). This initiative is designed to ...
(Date:1/14/2014)... Altadena, CA (PRWEB) January 14, 2014 ... Angeles County), California, and Montreal, Canada, has big ideas ... and non-fiction. The press's goals are to produce high-quality, ... e-books to support local businesses. , The first ...
Breaking Biology Technology:Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2Bitingduck Press Looks Forward to 2014 With Multimedia E-books 2
... March 7, 2012 Molecular Detection Inc. (MDI), ... the speed and accuracy of infectious disease diagnosis, ... million financing.  The funds are primarily being used ... the detection of sepsis and gastrointestinal (GI) diseases.  ...
... 2012   Proteonomix, Inc. (OTC/BB: PROT) announced today ... institutional investors, pursuant to which the Company has agreed ... stock at an aggregate purchase price of approximately $3.8 ... shares of Proteonomix common stock) and Series A, Series ...
... including Drexel University,s Dr. Yury Gogotsi has given the ... of the electrodes of supercapacitors the low-cost, lightweight ... many other applications. In a piece published in the ... and his collaborators from universities in France and England, ...
Cached Biology Technology:Molecular Detection Inc. Completes $1.5 Million Financing to Advance New Tests for Sepsis and GI Disorders 2Molecular Detection Inc. Completes $1.5 Million Financing to Advance New Tests for Sepsis and GI Disorders 3Molecular Detection Inc. Completes $1.5 Million Financing to Advance New Tests for Sepsis and GI Disorders 4Proteonomix Announces a Private Placement of $3.8 Million 2Proteonomix Announces a Private Placement of $3.8 Million 3Proteonomix Announces a Private Placement of $3.8 Million 4Drexel's Gogotsi and team advance understanding of energy storage mechanisms in Nature Materials 2
(Date:4/17/2014)... One day about eight years ago, Katia Silvera , ... her father were on a field trip in a mountainous ... they had never seen before. , Unable to identify it, ... The orchid turned out to be an unnamed species. So ... . , "Lophiaris" is the genus name, comprising about 40 ...
(Date:4/17/2014)... of forests in the Amazon help create tinderbox ... rapid and widespread forest loss during drought years, ... show that forests in the Amazon could reach ... forest fires lead to large-scale loss of trees, ... professor of geography, Penn State. , "We documented ...
(Date:4/17/2014)... Current Biology on April 17 have discovered little-known ... insects, which represent four distinct but related species in ... of an animal with sex-reversed genitalia. , "Although ... Neotrogla is the only example in which ... from Hokkaido University in Japan. , During copulation, which ...
Breaking Biology News(10 mins):Orchid named after UC Riverside researcher 2Orchid named after UC Riverside researcher 3Drought and fire in the Amazon lead to sharp increases in forest tree mortality 2Drought and fire in the Amazon lead to sharp increases in forest tree mortality 3In sex-reversed cave insects, females have the penises 2
... Mannitol, a sugar alcohol produced by fungi, bacteria, and algae, ... sweetener is also used in the medical field it,s ... excess fluids and used during surgery as a substance that ... drugs. Now Profs. Ehud Gazit and Daniel Segal of ...
... populations are declining worldwide and a major cause is a ... two-year study shows they can also die from this pathogen, ... that just spreads the disease. When researchers raised the ... least one strain of this pathogen, Batrachochytrium dendrobatidis , ...
... SAN FRANCISCO (June 16, 2013)Male mice who were fed a ... offspring who also had higher levels of body fat, a ... primarily in male offspring, despite their consumption of a low-fat ... Endocrine Society in San Francisco, Calif. , "We,ve identified a ...
Cached Biology News:Artificial sweetener a potential treatment for Parkinson's disease 2Bullfrogs may help spread deadly amphibian fungus, but also die from it 2Obese male mice father offspring with higher levels of body fat 2
... with Gateway™ Technology is designed to ... polyhedrin promoter. The expression cassette is ... DNA) by site-specific transposition (Tn7) in ... bacmid DNA is then used for ...
Porcine parvovirus and mycoplasma tested. Contains 0.5 g/L of trypsin (1:250) and 0.2 g/L of EDTA4Na in Hanks' Balanced Salt Solution without CaCl2 MgCl2 6H2O and MgSO4 7H2O. Contains phenol red....
Immunogen: Recombinant full length protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Immunogen: Synthetic peptide: R(89) L A F T V D D N P T K P T T R Q R Y(107) Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products: