Navigation Links
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

NA and 1.6 g of YEp352 plasmid; 2 x 108 cells were spread on 100 mm agar plates; 5 plates were shot for each experimental condition; the bombardment chamber was reduced to 28 Hg vacuum and cells were bombarded at Target Level 2 at a helium pressure of 1,1001,300 psi.

Nuclear transformation was quantitated by counting Ura+ transformants 5 days post-bombardment following growth of transformed cells on uracil-deficient media. Ura+ transformants were then analyzed for mitochondrial transformation by mating to S. cerevisiae strain 161 on glucose-containing media (Anziano & Butow, 1991; Butow, et al., 1996). Mitochondrial transformants were identified by replica plating these colonies onto media containing glycerol. Results are summarized in Table 4. Nuclear transformation is quite efficient using either 0.6 gold particles or M5 tungsten to deliver the DNA; there is a greater than 10- fold decrease in the number of transformants when 1 gold particles are substituted for 0.6 gold particles. In these experiments, mitochondrial transformants were only isolated when sub-micron sized gold particles were used. While mitochondrial transformants have been isolated using larger tungsten particles, results with those particles were extremely erratic in our hands, even in terms of nuclear transformation. Similar results were found when bombardment was performed with the Ura+ and COXI sequences on a single plasmid (results not shown).


Conclusions
Results presented here show that particle size is an important factor for optimizing Biolistic transformation. Maximum stable transformation of cellular organelles (chloroplasts and mitochondria
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Superior Gene Silencing Using Adenoviral Vectors
2. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Efficient Transfection of Neurospora Crassa
10. Efficient Transfection of Neurospora Crassa
11. Efficient Recovery of Ultrapure Plasmid DNA
Post Your Comments:
(Date:8/28/2015)... Aug. 28, 2015 /PRNewswire/ - RepliCel Life Sciences ... medicine company focused on the development of autologous ... quarter ended June 30, 2015, corporate highlights, and ... report are available at www.sedar.com ,  ... "RepliCel expects to launch its RCS-01 ...
(Date:8/28/2015)... ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in early ... excellent customer service. Images such as the Fonz in a lab coat and Large ... to the humdrum spectroscopy field. , FireflySci is proud to be the only ...
(Date:8/27/2015)... ... August 27, 2015 , ... Capital Edge Consulting , ... problems, is pleased to announce their ranking as one of the top 5000 companies ... fastest-growing private companies across the nation. , “It is truly an honor to be ...
(Date:8/27/2015)... - Portage Biotech Inc. ("Portage") (OTC Market: PTGEF, ... Company Limited (Biohaven), announced today that dosing has ... a glutamate modulating agent. Biohaven filed an investigational ... clearance from the U.S. Food and Drug Administration ... 54% equity interest in Biohaven, a private company. ...
Breaking Biology Technology:RepliCel Reports Second Quarter 2015 Financial Results 2RepliCel Reports Second Quarter 2015 Financial Results 3RepliCel Reports Second Quarter 2015 Financial Results 4RepliCel Reports Second Quarter 2015 Financial Results 5Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4
... will provide human safety and, pharmacodynamic ... to announce that its novel Innate Defense Regulator (IDR) ... time on April 27, 2009. The healthy volunteers were ... being performed for Inimex in Montreal with the approval ...
... confirms durable pain palliation as an acceptable primary endpoint, ... of market approval,BOTHELL, WA, April 28 /PRNewswire-FirstCall/ - OncoGenex ... that the company has reached an agreement with the ... of a second Phase 3 registration trial of OGX-011, ...
... SciLIMS Samples and Storage Management Product Line , ... ... , the premier provider of laboratory supplies and instrumentation to academic, ... new set of storage and sample management solutions. E&K is ...
Cached Biology Technology:Inimex Pharmaceuticals begins First Clinical Study of an Innate Defense Regulator 2OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer 2OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer 3OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer 4OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer 5OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer 6E&K Scientific Announces Partnership with Sciformatix 2
(Date:8/18/2015)... , Aug. 17, 2015 Research and ... of the "Global Biometric Authentication & Identification ... Estimation & Forecast, 2015-2020" report to their ... and identification systems market is expected to grow ... to 2020 and generate over $25 billion (approximately) ...
(Date:8/12/2015)... Aug. 12, 2015  Synaptics Inc. (NASDAQ: ... solutions, today announced that four of its fingerprint ... have officially been named FIDO Certified™ by the ... the certification, Synaptics, Natural ID™ fingerprint solutions are ... Framework (UAF) standard and are interoperable among other ...
(Date:8/6/2015)... and TELTOW, Germany , August ... Siggraph Conference, SensoMotoric Instruments (SMI) shows the ... , based on Epson,s Moverio BT-200 see-through ... platform. With this new solution, unprecedented quality and efficiency ... interaction with context-sensitive displays. For the first time, professionals ...
Breaking Biology News(10 mins):Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3
... comparing breastfed and formula fed infants across time showed ... than the potential risks associated with infant exposure to ... breastmilk, according to a report published in the December ... , a peer-reviewed journal published by Mary Ann Liebert, ...
... Clemson University professor of physics and astronomy Apparao Rao ... Society. Fellows are elected by their peers for outstanding ... than one half of 1 percent of the membership. ... carbon nanotubes and for elucidating the properties of carbon ...
... Unparalleled warming over the last few decades has triggered ... Western European lakes, say researchers at Queen,s University and ... reports that striking changes are now occurring in many ... rapidly warming Arctic, although typically many decades later. The ...
Cached Biology News:Benefits of breastfeeding outweigh risk of infant exposure to environmental chemicals in breastmilk 2Study links ecosystem changes in temperate lakes to climate warming 2
... peptidomimetics built on the flexible and ... These microarrays are available as part ... Service. Custom Probe Content ... specified. Thousands of customer specified peptide ...
... OPA1-01009, OPEP-01009 is a 16 amino acid (amino acids 3-18) ... (DFF). The sequence of this peptide is (amino to ... S - V - K - L - R - ... R - K(18). This peptide may be used ...
... standard microarray image analysis software because of ... tools, visualizations, automation capabilities, performance and ease ... every GenePix scanner, and it also imports ... types of arrays. A wide range of ...
... Applications Package Superior Protein Detection and ... ProXPRESS 2D Proteomic Imaging System is a ... and general life science research applications. ProXPRESS ... of all dyes in the UV and ...
Biology Products: