Navigation Links
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

omonas reinhardtii with an arginine-requiring point mutation in the ARG7 gene encoding argininosuccinate lyase (strain CC-48) or a non-photosynthetic, acetate-requiring deletion mutation in the atpB gene (strain CC-373) were used for assaying nuclear and chloroplast transformation, respectively. For nuclear transformation, CC-48 cells were bombarded with plasmid P-389 (pARG7.8; Debuchy, et al., 1989) containing the wild-type ARG7 argininosuccinate lyase gene from C. reinhardtii; for chloroplast transformation, CC-373 cells were bombarded with plasmid P-437 (BamH I chloroplast fragment 10 cloned into pBluescript KS+) containing the wild type atpB gene from C. reinhardtii. Bombardment conditions were as follows (Boynton & Gillham, 1993): 0.6 mg of 0.6 or 1 gold or M10 tungsten (average size = 0.7 ) particles were coated with 1.5 g of CsClpurified P-389 (for nuclear transformation) or P-437 plasmid (for chloroplast transformation); 4 x 107 log-phase cells in 0.1% top agar were spread on 100 mm agar plates; 10 plates were shot for each experimental condition; cells were placed at Target Level 2; the bombardment chamber was reduced to 26 Hg vacuum and cells were bombarded at a helium pressure of 1,300 psi. Following bombardment, cells from each plate were respread onto two plates of selective media: nuclear transformants were re-plated onto arginine-free media and chloroplast transformants were re-plated onto acetate-free media.

For nuclear transformation, arg+ colonies appeared within 7 days post-bombardment, while photosynthetic colonies resulting from chloroplast transformation were visible 35 days postbombardment. In these experiments, final scoring of all transformants was performed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Superior Gene Silencing Using Adenoviral Vectors
2. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Efficient Transfection of Neurospora Crassa
10. Efficient Transfection of Neurospora Crassa
11. Efficient Recovery of Ultrapure Plasmid DNA
Post Your Comments:
(Date:2/27/2015)... , Feb. 27, 2015  Pfenex Inc. (NYSE MKT: ... of biosimilar therapeutics, today announced that it will be ... Marquis on March 2 nd at 3pm PST. Patrick ... a panel discussing the current state of the biosimilar ... . For more information on CALBIO ...
(Date:2/26/2015)... NEW YORK , Feb. 26, 2015 ... commenced Factual Stock Report coverage on MabVax Therapeutics ... Inc. (MBVX) is a clinical stage biotechnology company focused ... address unmet medical needs in the treatment of cancer. ... products based on the protective immune responses generated by ...
(Date:2/26/2015)... , Feb. 26, 2015   Regulus Therapeutics ... company leading the discovery and development of innovative medicines ... , Ph.D., President and Chief Executive Officer of Regulus, ... Company 35 th Annual Healthcare Conference on Wednesday, ... is being held at the Boston Marriott Copley Place.  ...
(Date:2/26/2015)... 2015  NuGene International, Inc. ("NuGene") (OTCQB: NUGN), ... and hair rejuvenation, has announced that it has ... for investor relations and strategic communications. ... and diversification strategies – and we need to ... stated Ali Kharazmi CEO of NuGene when announcing ...
Breaking Biology Technology:S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on MabVax Therapeutics Holdings Inc. in S&P Capital IQ Factual Stock Reports 5Regulus to Present at Cowen and Company 35th Annual Healthcare Conference 2Regulus to Present at Cowen and Company 35th Annual Healthcare Conference 3NuGene International, Inc. Selects Capital Market Relations for Strategic Investor Relations 2NuGene International, Inc. Selects Capital Market Relations for Strategic Investor Relations 3
... Company, FRAZER, Pa., Feb. 8 Cephalon, Inc., ... Counsel &,Secretary, John E. Osborn, has decided to resign ... has agreed to remain as an advisor,to the company ... been an invaluable member of our senior executive team ...
... REDWOOD CITY, Calif., Feb. 8 Genomic Health,Inc. (Nasdaq: ... Health,s,President and Chief Operations Officer, will present at the ... Feb. 12, 2008 at 10:15 a.m. Eastern,Time., To ... visit the Investor Relations section of Genomic Health,s,website at ...
... InterMune, Inc.,(Nasdaq: ITMN ) today announced results from operations ... company also provided 2008,financial guidance and milestones for its key ... InterMune reported a net loss for the fourth quarter of ... net loss of $21.5 million, or,$0.64 per share, in the ...
Cached Biology Technology:Cephalon General Counsel John E. Osborn to Resign Position 2Cephalon General Counsel John E. Osborn to Resign Position 3InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 2InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 3InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 4InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 5InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 6InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 7InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 8InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 9InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 10InterMune Announces Fourth Quarter and Full Year 2007 Financial Results and Provides 2008 Guidance 11
(Date:2/9/2015)... RICHARDSON, Texas , Feb. 9, 2015  Lintec ... novel fabrication methods for carbon nanotube (CNT) macrostructures, including ... Texas at Dallas (UTD). Leveraging the vast ... Tokyo, Japan , Lintec of America ... Richardson, TX , focusing on scaling ...
(Date:2/5/2015)... SAN DIEGO , Jan. 26, 2015   Epic ... treatment of cancer, today announced that Murali Prahalad , ... Personalized Medicine World Conference (PMWC) 2015: Silicon Valley, ... Mountain View, Calif. on January 26-28, ... entitled, "CTCs Come of Age as Biomarkers." Last year, Epic ...
(Date:2/5/2015)... -- New Market Research Reports Title Global ... 2020 has Been Added to GrandViewReseach.com Report Database ... reach USD 5.10 billion by 2020, according to a ... help identify the site of tissue damage in case ... in medical imaging applications. They have been extensively used ...
Breaking Biology News(10 mins):Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 2Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 3Lintec announces exclusive license to commercialize innovative carbon nanotube technology from the University of Texas at Dallas 4Epic Sciences to Present at Personalized Medicine World Conference 2015 on the Use of Circulating Tumor Cells as Cancer Biomarkers 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3
... date of 130 North American tree species concludes that ... northward by hundreds of kilometers and shrink the ranges ... McKenney of the Canadian Forest Service and his colleagues, ... McKenneys study is based on an extensive data-gathering effort ...
... Trust researchers have identified a key gene involved in the ... mostly women. The lead researcher behind the study has called ... to further study the underlying causes of the disease. ... autoimmune disease which frequently causes skin rash, joint pains and ...
... able to differentiate metastatic cancer cells from normal cells ... softness of the cells. The study, published Dec. ... journal Nature Nanotechnology, represents one of the first times ... cancer patients and apply nanotechnology to analyze them and ...
Cached Biology News:Climate change predicted to drive trees northward 2Lupus gene finding prompts call for more DNA samples 2Using nanotechnology, UCLA researchers discover cancer cells 'feel' much softer than normal cells 2Using nanotechnology, UCLA researchers discover cancer cells 'feel' much softer than normal cells 3Using nanotechnology, UCLA researchers discover cancer cells 'feel' much softer than normal cells 4
... are hydrophobic cover slips for in ... genomic arrays on glass slides., ... Hybri-slips do not adsorb probes. , ... to use. , Avoid RNA degradation-Hybri-slips ...
...
Experion spin filters are used to filter the gel matrix and gel stain solution prior to use with the Experion automated electrophoresis system. Supplied as 10 spin filters....
...
Biology Products: