Navigation Links
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

omonas reinhardtii with an arginine-requiring point mutation in the ARG7 gene encoding argininosuccinate lyase (strain CC-48) or a non-photosynthetic, acetate-requiring deletion mutation in the atpB gene (strain CC-373) were used for assaying nuclear and chloroplast transformation, respectively. For nuclear transformation, CC-48 cells were bombarded with plasmid P-389 (pARG7.8; Debuchy, et al., 1989) containing the wild-type ARG7 argininosuccinate lyase gene from C. reinhardtii; for chloroplast transformation, CC-373 cells were bombarded with plasmid P-437 (BamH I chloroplast fragment 10 cloned into pBluescript KS+) containing the wild type atpB gene from C. reinhardtii. Bombardment conditions were as follows (Boynton & Gillham, 1993): 0.6 mg of 0.6 or 1 gold or M10 tungsten (average size = 0.7 ) particles were coated with 1.5 g of CsClpurified P-389 (for nuclear transformation) or P-437 plasmid (for chloroplast transformation); 4 x 107 log-phase cells in 0.1% top agar were spread on 100 mm agar plates; 10 plates were shot for each experimental condition; cells were placed at Target Level 2; the bombardment chamber was reduced to 26 Hg vacuum and cells were bombarded at a helium pressure of 1,300 psi. Following bombardment, cells from each plate were respread onto two plates of selective media: nuclear transformants were re-plated onto arginine-free media and chloroplast transformants were re-plated onto acetate-free media.

For nuclear transformation, arg+ colonies appeared within 7 days post-bombardment, while photosynthetic colonies resulting from chloroplast transformation were visible 35 days postbombardment. In these experiments, final scoring of all transformants was performed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Superior Gene Silencing Using Adenoviral Vectors
2. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Efficient Transfection of Neurospora Crassa
10. Efficient Transfection of Neurospora Crassa
11. Efficient Recovery of Ultrapure Plasmid DNA
Post Your Comments:
(Date:4/24/2015)... Telbec/ - Mirego, leader in mobile strategies and solutions, is ... app extensions for the all new Apple Watch. Made in ... and MediaMiser, these extensions will reinvent the way users receive ... wrist use.  Available today in ... tactile smartwatch that makes for an excellent buddy for business, ...
(Date:4/23/2015)... Athena San Diego, an organization that ... in San Diego’s technology and life science sectors, will ... at their Gala on April 30, 2015 from 5-9pm ... http://athenasd.org/events/ , The Pinnacle Awards recognize individuals ... vision of fostering networking, risk taking and diversity of ...
(Date:4/23/2015)... 2015  Neurocrine Biosciences, Inc. (Nasdaq: NBIX ) ... quarter 2015 results before the Nasdaq market opens on ... conference call and webcast to discuss its financial results ... 2015 at 8:00 a.m. Eastern Time (5:00 a.m. Pacific ... call by dialing 866-952-1906 (US) or 785-424-1825 (International) using ...
(Date:4/23/2015)... Prospects for Leading Companies in OTC, Rx ... We predict the global healthcare packaging market ... and will grow at a CAGR of 6.3% between 2015 ... world and increasing functional requirements for healthcare packaging products are ... packaging market. A global ageing population, changing lifestyles ...
Breaking Biology Technology:Familiprix, OMSignal and MediaMiser apps available today for Apple Watch 2Athena Pinnacle Awards to Honor Executives, Corporations and Educators Working to Promote Women Leaders 2Neurocrine Biosciences Announces Conference Call and Webcast to Report First Quarter 2015 Results 2Healthcare Packaging Market Forecast 2015-2025 2Healthcare Packaging Market Forecast 2015-2025 3Healthcare Packaging Market Forecast 2015-2025 4Healthcare Packaging Market Forecast 2015-2025 5Healthcare Packaging Market Forecast 2015-2025 6Healthcare Packaging Market Forecast 2015-2025 7Healthcare Packaging Market Forecast 2015-2025 8Healthcare Packaging Market Forecast 2015-2025 9Healthcare Packaging Market Forecast 2015-2025 10
... Inclinix, Inc. has announced today that it has ... (Nasdaq: DATA ) to offer the most ... device companies globally. , ... DATATRAK,s eClinical(TM) platform to enhance its services as a ...
... Md., April 30 /PRNewswire/ -- ... company developing novel, proprietary antibodies for the treatment of ... will host a conference call and audio webcast on ... (3:00 pm Central European Time) to discuss its financial ...
... Inc. (NASDAQ: AEZS , TSX: AEZ), a ... will announce its first quarter 2009 financial and operating ... The Company will host a conference call and webcast ... 3:30 p.m., Eastern Time. , Participants may access the ...
Cached Biology Technology:DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 2DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 3DATATRAK International, Inc. Forms Strategic Partnership with Inclinix, Inc. 4Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 2Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 3Micromet, Inc. to Host Conference Call and Webcast to Report First Quarter 2009 Financial Results on May 6, 2009 4
(Date:4/10/2015)... , April 10, 2015 Research ... of the "Security Competitive Profiles - NEC" ... , NEC will continue to supply a ... the market, with a company focus on the development ... Winning opportunities in the Asia-Pacific ...
(Date:4/2/2015)... April 2, 2015 At its 2015 ACMG ... City , the American College of Medical Genetics ... directors to its Board.  Members of the ACMG Board ... for forming and advancing its policies and programs. ACMG ... "It,s an eventful time in medical genetics ...
(Date:3/31/2015)... YORK , March 31, 2015  Elephant Talk ... "Company"), a global provider of Software Defined Network Architecture ... today announced total revenue of approximately $20.4 million for ... a restated $19.5 million for the year ended December ... calendar 2014, the Company had completed its multi-year transition ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... fourth largest honey producer and fifth largest honey exporter ... rural farmers in Mexico to quantify the genetically modified ... sale in Germany. Their results will appear Feb. 7 ... Roubik, senior staff scientist at the Smithsonian Tropical Research ...
... top predators to walk on land were not afraid to ... Toronto Mississauga study has found. Graduate student and lead ... of T Mississauga,s Department of Biology suggest that Dimetrodon ... and 272 million years ago, was the first terrestrial vertebrate ...
... (U.S.) predominance in science and technology (S&T) eroded further ... and South Korea--rapidly increased their innovation capacities. According to ... (NSB), the policy making body of the National Science ... Congress, the major Asian economies, taken together, now perform ...
Cached Biology News:Smithsonian reports GMO soybean pollen threatens Mexican honey sales 2'Steak-knife' teeth reveal ecology of oldest land predators 2US lead in science and technology shrinking 2US lead in science and technology shrinking 3US lead in science and technology shrinking 4
Recombinant Viral CCI/Fc Chimera, CF...
Rat Normal Multiple Tissue Array 1 slide...
EPH electrode, anode, 1. Category: Electrophoresis Systems & Standards & Reagents, IEF & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
GENOMIPHI HY KIT 1000 RXN, 1 EA. Category: Genomics DNA Amplification....
Biology Products: