Navigation Links
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

velocity, the more likely the particle will penetrate the cell; this is particularly important when using small particles. The chamber vacuum affects the velocity of the microparticles by reducing the drag on the particles as they travel toward the target tissue: the higher the chamber vacuum, the less the particles will be decelerated. The distance between the stopping screen and the target tissue has several effects on the transformation efficiency. The longer the travel distance, the greater the spread of the particles over the target and the less pronounced is the helium shock wave striking the cells. On the other hand, a longer travel distance results in reduced particle speed and a decreased likelihood that a particle will penetrate the target.

Because the likelihood of a subcellular-sized particle penetrating a cell is proportional to the kinetic energy of the particle, materials of high density have generally been preferred for use in biolistic technology. These compounds include tungsten, platinum, and gold. Of these, gold is favored because it is biologically inert and because spherical gold particles can be produced in a narrow size range. Physical parameters of gold particles are presented in Table 1. Note the nearly five-fold increase in the number of particles per gram for the 0.6 gold relative to the 1.0 gold. The importance of particle size and density on transient expression has been demonstrated for a variety of cell systems (Sanford, et al., 1993). Here we present data to demonstrate that for stable expression in several biological systems, particle bombardment is more efficient using 0.6 gold particles than using particles of larger size.


Methods and R
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. Superior Gene Silencing Using Adenoviral Vectors
2. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
3. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
4. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
5. PCR-Grade Nucleotides Larger Pack Sizes Now Available
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
8. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
9. Efficient Transfection of Neurospora Crassa
10. Efficient Transfection of Neurospora Crassa
11. Efficient Recovery of Ultrapure Plasmid DNA
Post Your Comments:
(Date:5/5/2015)... ImmunoCellular Therapeutics, Ltd. ("ImmunoCellular") (NYSE MKT: ... and Chief Executive Officer, will present live at ... May 7, 2015     TIME:  11:30 am ET/8:30 am ... the red "register/ watch presentations" button ... where investors are invited to ask the company ...
(Date:5/5/2015)... Washington, and WASHINGTON, D.C., USA (PRWEB) May 05, 2015 ... on U.S. export regulations covering a wide range of ... industry say are hampering global competitiveness of U.S. industry. ... host a webinar on 12 May on ... XII, which governs the commodities covered by International Traffic ...
(Date:5/5/2015)... Research and Markets ( http://www.researchandmarkets.com/research/x5gjj3/clinical ... "Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services ... Global Version" report to their offering. ... of molecular diagnostics and pharmacogenics hold the promise ... that is moving out of the national and ...
(Date:5/5/2015)... , May 5, 2015 CytomX, a ... cancer, today announced that Sean McCarthy , D. ... Suisse Antibody Day in New York ... Dr. McCarthy will present an overview of CytomX,s Probody ... drug conjugate programs. About CytomX Therapeutics ...
Breaking Biology Technology:ImmunoCellular Therapeutics to Webcast Live at VirtualInvestorConferences.com on May 7 2Comments needed now from photonics industry on revisions of U.S. export rules 2Comments needed now from photonics industry on revisions of U.S. export rules 3Comments needed now from photonics industry on revisions of U.S. export rules 4Global Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market Trends and Forecast 2015 to 2019 2
... non-profit cooperative that networks Wisconsin institutions, took different directions ... eWeek last September, the magazine has reported. ... more than 30 respondents and choosing six for evaluation, ... flexible enough to provide anti-spam services to all of ...
... in the Dark Ages, where marks were burned onto cattle. On ... made a solid impression and provides a daily dose of comfort. ... vanity offers a security blanket. A warm fuzzy feeling when a ... body just craves to carry something with a logo, color and ...
... the last two years of writing this column, I have become ... in the Midwest. Part of the challenge of this column has ... ,Though many Midwest life science companies are well known, often a ... Rarely, however, has been it the case where I have never ...
Cached Biology Technology:WiscNet members choose anti-spam products for different needs 2The black hole of corporate branding 2The black hole of corporate branding 3The black hole of corporate branding 4Bio-Logic Systems, another Midwest life-science firm, flies under the radar 2Bio-Logic Systems, another Midwest life-science firm, flies under the radar 3Bio-Logic Systems, another Midwest life-science firm, flies under the radar 4
(Date:4/13/2015)... YORK , April 13, 2015 ... by TechSci Research, "Global Biometrics Market Forecast & Opportunities, ... register a CAGR of around 14% till 2020. ... governments for large scale implementation and review of ... and launch of new products with greater efficiency, ...
(Date:4/10/2015)... DUBLIN , April 10, 2015 ... the addition of the "Security Competitive Profiles - ... (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , NEC will continue to ... solutions to the market, with a company focus on ... solution anticipated. Winning opportunities in the ...
(Date:4/8/2015)... 2015  Infinisource, a leading provider of SaaS-based Human ... a leading supplier of biometric identification and security ... most advanced biometrically-enabled time clock, the companies announced ... is setting a bold, new standard for the ... small and mid-size employer. When connected to the ...
Breaking Biology News(10 mins):Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2NEC Security Competitive Profile 2015 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3
... February 20, 2013 As part of an ongoing effort ... scientists, led by crude oil producer Sapphire Energy, Inc. ... identified the morphology, ultrastructure, and life history of A. ... parasites. Their findings are detailed in "Characterization of Amoeboaphelidium ...
... (OTC Markets: MDBX) ( www.medboxinc.com ), announced that their patented ... Colorado marijuana task force,s recommendations regarding "pot tourism." ... made available to tourists over the age of 21, though ... Representative Dan Pabon (D), who sits ...
... a substance found in red grapes and red wine, may ... according to a published laboratory study from Henry Ford Hospital ... less likely to suffer the long-term effects of noise-induced hearing ... for a long period of time. "Our latest study ...
Cached Biology News:Scientists publish analysis of algae parasite impact on algae biofuel in PLOS ONE 2Colorado Marijuana Task Force Gives Green Light To "Pot Tourism" 2Study: Resveratrol shows promise to protect hearing, cognition 2