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Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

a biological assay using the particles in bombardment and measuring transient or stable expression. Together, these assays are designed to assure lot-to-lot consistency of gold particles for Biolistic applications.


Bibliography
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3. Boynton, J. E. and Gillham, N. W., Genetics and transformation of mitochondria in the green alga Chlamydomonas, Meth. Enzymol., 264, 279-296 (1996).

4. Butow, R. A. and Fox, T. D., Organelle transformation: shoot first, ask questions later, Trends in Biochem., 15, 465-468 (1990).

5. Butow, R. A., Belcher, S. M., Moran, J. V., Henke, R. M. and Perlman, P. S., Transformation of S. cerevisiae mitochondria using the biolistic gun, Meth. Enzymol., 264, 265-278 (1996).

6. Debuchy, R., Purton, S. and Rochaix, J.-D., The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus, EMBO J., 8, 2803-2809 (1989).

7. Goff, S. A., Klein, T. M., Roth, B. A., Fromm, M. E., Cone, K. C., Radicella, J. P. and Chandler, V. L., Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues, EMBO J., 9, 2517-2522. (1990).

8. Klein, T. M., Goff, S. A., Roth, B. A. and Fromm, M. E., Applications of t
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