Navigation Links
Structural Analysis of Glycosylated Peptides in Complex Mixtures with ,,, Ion Trap MSn

br>
When all of the glycosylated peptides were identified and combined with the unmodified peptides, the sequence coverage obtained in the experiment was increased to greater than 90%. Figure 8. MSn on the LCQ ion trap mass spectrometer confirms predicted sequence, linkage site and glycostructure of an rt-PA peptide. The precursor ion in glycostructure 211 was determined using MS/MS. To determine oligosaccharide structure, glycostructure 210 was selected for further fragmentation using MS3. To further confirm the linked peptide sequence and the site of attachment, ion m/z = 1333 was trapped and fragmented further (MS4).
 Conclusions The Thermo Finnigan LCQ Deca XP ion trap mass spectrometer coupled with TurboSEQUEST protein identification software is an extremely powerful tool for the elucidation of post-translational modifications of proteins. In this case, the combination of Data Dependent MSn and Dynamic Exclusion have been used to quickly generate results which are more comprehensive than those provided by traditional approaches to glycoprotein analysis. While MS/MS can give limited oligosaccharide structure information, MSn can unambiguously confirm complete structure and the sites of attachment. This is yet another example of why the LCQ is an essential tool for every proteomics laboratory.
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Structural Determination of Flavonoids Using MSn
2. Continuous-Elution Electrophoresis for Purification of the Baculovirus-Expressed Coronavirus Structural Proteins, Rev A
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. Map and Link Human Genetic Disorders with SSLP Analysis
6. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
7. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
11. Agilent 2100 Bioanalyzer Automated Analysis System
Post Your Comments:
(Date:8/28/2008)...- ViroPharma Incorporated,(...o, president and chief,executive officer of ViroPh...008 Healthcare Conference at 8:00 A.M. ET on Thurs...ld at the Four Seasons Hotel in Boston, MA., Viro...resident,corporate communications of ViroPharma w...
(Date:8/28/2008)...st 28 /- ThromboGenics NV,(...ocused on innovative,treatments for eye disease, v...iness update and its financial results for the six...irst half of 2008, ThromboGenics has achieved a nu...ompany to successfully pursue the,next stage of it...
(Date:8/28/2008)...ewswire-FirstCall/ -- SulphCo, Inc. (the "Company"... with a patented ultrasound,process to desulfurize...d,S. Zeidman to the Company,s Board of Directors.,...,s Board of Directors,",said Dr. Larry D. Ryan, Ch...l benefit from his extensive experience, both in t...
(Date:8/28/2008)...ust 28 /- Strong organic,gr...h product offerings.,The development of Exiqon,s f...rofiling remains on track for year end launch., ...r-earlier period to DKK 28 million, totaling DKK ...Product sales in Q2 2008 increased by 239% on the ...
Breaking Biology Technology:ViroPharma to Present at Three September Healthcare Conferences 2ThromboGenics Announces Half Year Results 2008 2ThromboGenics Announces Half Year Results 2008 3ThromboGenics Announces Half Year Results 2008 4ThromboGenics Announces Half Year Results 2008 5SulphCo Adds New Board Member 2Exiqon A/S: Interim Report for the Period 1 January - 30 June 2008 (Unaudited) 2
...ucks customers in Wisconsin can order wireless In...han 3,100 company-owned Starbucks stores nationwid... HotSpot service for patrons with Wi-Fi-enabled n...ss Web by activating a T-Mobile HotSpot account. V...imited subscriptions, DayPass and pay-as-you-go pl...
...ell, is a powerful key to highly successful market... And what, if done poorly, is an enormous marketin... delegate will have a direct impact on how effecti.... In this article I will share some delegation tip...tion marketing efforts. , , Why delegate , ,De...
... Tour de France s fourth stage Wednesday, Lance Ar... the lead. That must make the designers at Trek B...ding. , ,Founded in 1976 in Waterloo, Wisconsin, t...tal with bicycles from the beginning, and it is no...xth consecutive victory for the team. , ,Treks new...
...drens Hospital of Wisconsin is helping to heal he...y freezing the tissue causing the abnormality. , ,...h Technologies reaches temperatures as low as min... the catheterization laboratory at Childrens Hospi...gist guide the catheter through a vein into the pa...
Other Biology Technology:Fire up your marketing by delegating 2Armstrongs new Trek modeled on AMD 64-bit processors 2
(Date:8/28/2008)...he way breakfast eggs conveniently slide off of Te...e pan. Indeed, the carbon-fluorine bond at the hea... in clothing, lubricants, refrigerants, anesthetic... the very strength of the C-F bond that makes it u...able greenhouse gas effects that persist in nature...
(Date:8/28/2008)... use less energy may soon be possible with new cer...al scientists responsible, this same material coul...rently, food heated in a microwave loses heat to t... microwaves," says Sridhar Komarneni, distinguishe...tural Sciences at Penn State. "The plates are stil...
(Date:8/28/2008)...enous magnesium sulfate supplementation before pre...ral palsy in half, according to research led by Un...an Dwight Rouse, M.D., and published in the Aug. 2..., Cerebral palsy refers to a group of neurologic...ture and limiting activity. The causes of cerebral...
(Date:8/27/2008)...management agencies are proud to announce the Firs..., in Visalia, California at the Visalia Convention... the symposium is "Agents of Change Affecting the ...g together premier scientists for what will be an ...., The science symposium will be organized around...
Breaking Biology News(10 mins):Breaking harmful bonds 2Ceramic material revs up microwaving 2Common treatment to delay labor decreases preterm infants' risk for cerebral palsy 2Common treatment to delay labor decreases preterm infants' risk for cerebral palsy 3Southern Sierra Science Symposium planned for Visalia 2Southern Sierra Science Symposium planned for Visalia 3Southern Sierra Science Symposium planned for Visalia 4Southern Sierra Science Symposium planned for Visalia 5Southern Sierra Science Symposium planned for Visalia 6Southern Sierra Science Symposium planned for Visalia 7Southern Sierra Science Symposium planned for Visalia 8United BioSource Corporation and CogState Ltd Form Strategic Partnership to Support Cognitive Assessment in Clinical Research 6948 1United BioSource Corporation and CogState Ltd Form Strategic Partnership to Support Cognitive Assessment in Clinical Research 6948 2United BioSource Corporation and CogState Ltd Form Strategic Partnership to Support Cognitive Assessment in Clinical Research 6948 3United BioSource Corporation and CogState Ltd Form Strategic Partnership to Support Cognitive Assessment in Clinical Research 6948 4Colorado Rocky Mountain Region 3A A Geological Cornucopia 4338 1Caltech scientists awarded 2420 million to Power the Planet 4336 1Caltech scientists awarded 2420 million to Power the Planet 4336 2NeurogesX to Report Second Quarter 2008 Financial Results 6946 1NeurogesX to Report Second Quarter 2008 Financial Results 6946 2NeurogesX to Report Second Quarter 2008 Financial Results 6946 3
...utate genes and large, non-gene pieces of DNA has ...They tested the method by mutating a gene involved...tail and limbs in the mutant mouse on the left, co...right. The new method promises to speed efforts to...trol genes to cause human defects and diseases. Cr...
...tory at the University of Central Florida is provi...g glaucoma, an eye disease that can cause blindnes... nanoparticle can safely get past the blood-brain ...drug delivery,” said Sudipta Seal, an engineering ...rials Processing and Analysis Center and the Nanos...
...istence without thousands of proteins performing m...ng proteins can cause disease, the study of protei...nt of drugs and treatments for numerous disorders....iabetes paved the way for the development of a tre...normous research efforts led by scientists worldwi...
...ne in advance of its July 1st publication date, Dr... Salk Institute and HHMI) demonstrate that endosom...as well as in animals. , "These findings will infl...endosomes and hopefully help us to understand why ...ead author of the paper, Dr. Niko Geldner. , En...
Other Biology News:Mutating the entire genome 2Mutating the entire genome 3Mutating the entire genome 4Mutating the entire genome 5UCF nanoparticle offers promise for treating glaucoma 2Weizmann Institute scientists develop a general 'control switch' for protein activity 2Weizmann Institute scientists develop a general 'control switch' for protein activity 3
Anti-DNA Polymerase a, Human Description: 50 l Research Focus: cancer & cell proliferation Storage: 4C Shipping Temperature: 4C
Vitronectin Immunogen: Purified, full length, native human protein. Storage: -20 C, Avoid Freeze/Thaw Cycles
...essible to anyone in the lab. Novice and experts a... on-demand cytometry systemsthe Guava EasyCyte Plu...act for higher throughput, multi-color detection. ... counts, 6 parameters (4 fluorescent colors, 2 lig...
Horseradish Peroxidase (HRP) Conjugation Kit For labeling Antigens/Antibodies (Reaction Time 2 hr) from Alpha Diagnostic International Inc
Biology Products: