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Streamline Your siRNA Transfections

NEW

siPORT NeoFX Transfection Agent Promotes High Reproducibility and Efficiency


Fast--transfect cells as they are plated (reverse transfection), saving a day
Reproducible--produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
Versatile--works with a broad range of cell lines
Efficient--performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
Powerful--performs with low cytotoxicity in the presence or absence of serum


Streamlined Transfection Protocol

siRNA transfection is traditionally a two-day procedure, making optimization a relatively slow process. siPORT NeoFX Transfection Agent was developed to refine siRNA transfection protocols. siPORT NeoFX lipid-based formulation can be used to efficiently transfect adherent cells just after trypsinization--without increased cytotoxicity. Just add siPORT NeoFX to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells (Figure 1). This new streamlined protocol, named "neofection," will save researchers a full day of valuable time when compared to traditional pre-plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection. From start to finish, successful gene silencing experiments can be completed i
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