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Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and,,,Discover New Genes


New GeneConnection discovery microarrays provide more than 4,000 unique human cDNA sequences

Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarraysII which are based on the technology published by Schena et al.,1 can be used for monitoring RNA expression levels of genes. The microarrays are configured on standard 25 mm x 75 mm glass microscope slides. Each slide contains more than 4,000 human cDNA sequences spotted in a 44 x 96 grid.

Discovery Clone Collection: A High Degree of Novelty

The human cDNA spotted on the GeneConnection discovery-3 microarrays are from Stratagenes GeneConnection discovery clone collection. This collection consists of clones from our innovative libraries and contains a high number of clones that do not have significant nucleotide homology to clones in public databases.* Therefore using Stratagenes human cDNA microarrays allows gene expression profiling to be combined with gene discovery to maximize results.

All of the cDNAs in the discovery clone collection, including those used to create the human cDNA microarrays, are available. Approximately 300 to 600 bases at the 3 end of each of these cDNAs have been sequenced in a single pass. The sequences were compared to those in the public databases using the Basic Local Alignment Search Tool (BLAST) technology. Clones having significant nucleotide homology to one or more characterized sequences in the public databases are assigned a name, accession number, and UniGene number. Clones having significant homology to one or more Expressed Sequence Tag (EST) sequences in the public databases are also assigned an EST number. Clones not having significant nucleotide homology to those in the public databases are identified as unknown o
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