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Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation

RNAlater Tissue Collection: RNA Stabilization Solution for Freshly Dissected Tissue and Cells.


Problem:
Successful RNA isolation requires rapid processing or appropriate storage of the tissue or cell samples. Tissue samples must be quickly dissected on ice, followed by immediate disruption in lysis buffer. Alternatively, they can be quickly frozen using liquid nitrogen or dry ice. Any delay in processing can result in decreased RNA yield and integrity.

Solution:
RNAlater is an aqueous, non-toxic tissue and cell storage reagent that stabilizes cellular RNA within intact, unfrozen tissue and cell samples. At the same time, it "freezes" the RNA expression pattern so that analysis of the RNA will reflect actual expression levels of message at the time of sample collection. Tissue pieces submerged in RNAlater can be stored at room temperature for 1 week, 4C for a month, or -20C to -80C indefinitely. RNAlater can also be added to cell pellets and even cells in media. Samples can be shipped across continents at ambient temperature or on wet ice. RNAlater is compatible with all RNA isolation methods examined. For an example of RNAlater's use with bacteria cells, see the article Preserving Campylobacter jejuni RNA for Microarray Analysis, at right.


RNAlater-ICE Frozen Tissue Transition Solution for Frozen Tissue
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