Navigation Links
Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry

Overview

Traditionally, MALDI-TOF mass spectrometry has been used very little for quantitative studies. Analysts have usually assumed that potential differences in ionization efficiency between analyte and internal standards and an inherent heterogeneity of the sample spot would limit MALDI-TOF mass spectrometry as a quantitative technique. In addition, conventional MALDI-TOF spectral acquisition has not allowed rejection of saturated peaks which, when averaged with unsaturated peaks, do not provide peak areas or heights suitable for quantitation. However, with the introduction of sophisticated software and high precision spotting robots, the use of MALDI-TOF mass spectrometry for small organic molecule and peptide quantitation has become a viable analytical procedure. Robotic or manual sample preparation combined with automated MALDI-TOF mass spectrometry, using single-shot averaging, is now a viable method for quantitation of small organic molecules and peptides.

Key Features

Automatic and intelligent spectra acquisition and evaluation

Acceptable calibration curve linearity from robotic or manual sample preparation

Visual Basic for Applications macro for quantitative analysis

Experimental Conditions

Small Organic Molecule Quantitation

Stock standard solutions were prepared in ethanol. Working analyte (4-acetoamidophenol) standard solutions were prepared by serial dilution of the stock analyte solutions with acetonitrile/water 50:50 (v/v). Working internal standard (theophylline) solutions were prepared directly with matrix solution (2 g/L DHB prepared in 50% acetonitrile/50% 0.1% TFA). Equal volumes of the working and internal standard solutions were mixed. The SymBiot I robot was used to spot 0.5 μL of each solution in triplicate onto the MAL
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer
2. Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. Comparison of Different Methods of mRNA Quantitation
5. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
6. Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
9. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
10. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
11. A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)
Post Your Comments:
(Date:7/28/2015)... , July 28, 2015  Moerae Matrix ... Phase 1 clinical trial with MMI-0100, a first-in-class ... being developed for pulmonary disorders characterized by inflammation ... United Kingdom , is a double-blind, ... evaluating the safety and tolerability of MMI-0100 when ...
(Date:7/28/2015)... Israel , July 28, 2015 ... medicine company utilizing its proprietary plant-based rhCollagen technology ... Shurtz has been appointed Senior Director of Business ... (Photo: http://photos.prnewswire.com/prnh/20150728/246955 ) , ... in marketing, regulatory and business strategy. Prior to ...
(Date:7/27/2015)... ... July 27, 2015 , ... This is a professional ... a basic overview of the industry including definitions, classifications, applications and industry chain ... trends, competitive landscape analysis, and key regions development status. Development policies and plans ...
(Date:7/27/2015)... , ... July 27, 2015 , ... ... international sales network Monday. The venture will bring the world’s most efficient gas ... provides state-of-the-art equipment and technologies to both the industrial and municipal markets. With ...
Breaking Biology Technology:Moerae Initiates Lipopolysaccharide (LPS) Challenge Study of Inhaled MMI-0100 for Pulmonary Disease Following Encouraging Safety and Tolerability Observed in Single Ascending Dose Phase 1 Study 2CollPlant Names Shomrat Shurtz Senior Director of Business Development 22015 Biophotonics Market Global Research 22015 Biophotonics Market Global Research 3BlueInGreen Brings Water Treatment Technology to Florida 2
... ... ... Agricultural improvements will play a key role in tackling Africa’s crucial problems of hunger ... during the World Economic Forum on Africa. , , , , ,“Local infrastructure that ...
... May 6, 2010 Oramed,Pharmaceuticals Inc. (OTCBB: ORMP), a developer ... Phase 2b non-FDA,clinical trial of its flagship oral insulin capsule, ORMD-0801. ... ... Africa evaluated responses of 29 Type 2 diabetes patients,to ORMD-0801. ...
... ... ... -- ProRepro today announced it has become an Autodesk authorized value-added reseller (VAR). ... California. ProRepro’s qualified staff demonstrates in-depth technical and industry knowledge and a commitment to ...
Cached Biology Technology:DuPont Leader: Agricultural Improvements Key to Meeting Growing Food, Water Needs in Africa 2Oramed Pharmaceuticals Reports Results in Phase 2b Trial of Oral Insulin Administration to Type 2 Diabetes Patients 2Oramed Pharmaceuticals Reports Results in Phase 2b Trial of Oral Insulin Administration to Type 2 Diabetes Patients 3ProRepro Becomes a Value-Added Reseller for Autodesk 2
(Date:7/23/2015)... Research and Markets ( http://www.researchandmarkets.com/research/rzzp3v/global_outlook_of ) ... Outlook of the Biometrics Industry 2015 " ... global biometrics market is likely to witness significant ... to the uptake of multiple technologies available. The ... the growth of biometrics in existing applications as ...
(Date:7/21/2015)... , July 21, 2015  NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile ... wallet, announces that it has filed provisional patent ... Method. This invention highlights next ... authorizes an account, but also the user using ...
(Date:7/20/2015)... , July 20, 2015  Acuity Market ... Report: The Convergence of Commerce and Privacy" forecasts ... mobile biometric apps will be downloaded to smart ... The mobile biometrics market is projected to generate ... revenue during the seven-year forecast period.    ...
Breaking Biology News(10 mins):Global Outlook of the Biometrics Industry 2015 2Global Outlook of the Biometrics Industry 2015 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4Biometrics on Smart Mobile Devices to Redefine Digital Identity with 12.9 Billion Biometric App Downloads between 2014 and 2020 2
... As most American families sit down to Thanksgiving dinner, a ... the largest animals on earth feasts on the smallest of ... mammals are known for their extraordinarily long dive times. Elephant ... a time by lowering their heartbeat and storing large amounts ...
... developing a model to explain the motion of atoms ... obstacle to understanding a key component of magnetic resonance. ... to better control of magnetic resonance imaging (MRI) and ... University in Columbus and three institutions in France--the Centre ...
... associate vice president for technology development at NJIT, has ... Science and Technology to assist faculty researchers with the ... to $50,000. The money, known as Gap grants, ... interesting idea and a commercial product. Sixteen ...
Cached Biology News:UBC researcher reveals humpback whales' dining habits -- and costs 2Dancing atoms now understood 2Dancing atoms now understood 3Dancing atoms now understood 4Moving new technologies from the lab to the marketplace 2Moving new technologies from the lab to the marketplace 3
... Acridinium NHS ester can be ... nucleic acids. The covalently bound ... chemiluminescence in the presence of ... proteins can be used as ...
... instrument designed to hold up to 6 strain ... and easy to use design ensures that cells ... cyclic stretching or compression. This unit allows you ... strain to produce 64 unique strain settings. The ...
... The Genome-pULSe Kit delivers a novel procedure ... (non-enzymatic) labeling of whole genome DNA from ... to provide a quick and highly reproducible ... Genome-pULSe consists of a REPLI-g Mini Kit ...
... may be superior to transfection in some ... with PULSin you can study lethal proteins ... of proteins in cells. Likewise, delivery of ... traditional RNA interference experiments. ,With PULSin ...
Biology Products: