Navigation Links
Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf ,,, cMaster RTplusPCR System

5 min Fixation of the RNA 0C 5 min Addition of master mix 2 (10 l) Primer annealing 45C 10 min cDNA synthesis 50C 50 min
Preparation of the PCR


Components Volume Final concentration in the reaction dNTP mix (each 10 mM) 2.0 l 400 M Forward primer D (100 M) 0.5 l 1 M Reverse primer D (100 M) 0.5 l 1 M Forward primer G (100 M) 0.5 l 1 M Reverse primer G (100 M) 0.5 l 1 M Right control primer (100 M) 0.5 l 1 M Left control primer (100 M) 0.5 l 1 M First-strand cDNA, see above 6.0 l Master mix 3 11.0 l RTplusPCR buffer with 25 mM Mg++ 5.0 l 1 x cMaster PCR enzyme mix (5 U/l) 0.5 l 0.05 U/l RNase-free water 33.5 l Master mix 4 39.0 l
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. RT-PCR Primers for the Study of Apoptosis
8. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
(Date:9/2/2014)... -- Technology Applications International Corporation (the "Company" or "NUUU")(OTC BB: ... Inc. has signed an additional exclusive licensing agreement with ... Johnson Space Center in Houston , ... Charles J. Scimeca , NUUU,s ... latest, exclusive agreement with NASA. In addition to our ...
(Date:9/2/2014)... 2, 2014  Xencor, Inc. (NASDAQ: ... monoclonal antibodies for the treatment of autoimmune diseases, ... the appointment of Debra Zack , M.D., ... of Lloyd Rowland , senior vice president, ... "Debra,s expertise in biologics clinical development and ...
(Date:9/2/2014)... 02, 2014 PMG Research, Inc ... clinical research site: PMG Research of Rocky Mount. Effective ... of the clinical research department at Boice-Willis Clinic ... in eastern North Carolina. This new site enhances PMG’s ... in Rocky Mount and the surrounding communities, this brings ...
(Date:9/1/2014)... Sept. 1, 2014 Reportlinker.com announces that ... its catalogue: India Solid Waste Management ... http://www.reportlinker.com/p02350445/India-Solid-Waste-Management-Vehicles-Market-Forecast-and-Opportunities-2019.html In ... main governing bodies responsible for complete handling and ... an average, around 135,000 MT solid waste is ...
Breaking Biology Technology:Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 2Technology Applications International Corporation Subsidiary Enters Into Additional Exclusive Agreement with NASA at a Signing Ceremony at the Johnson Space Center In Houston, Texas 3Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3PMG Research Announces the Formation of its Eleventh Site 2PMG Research Announces the Formation of its Eleventh Site 3India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 2India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 3India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 4India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 5India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 6India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 7India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 8
... , NEW YORK, Aug. 13 Cadus Corporation (OTC ... 30, 2009. , , Revenues were $0 for the second ... for the second quarter of 2009 was $103,330, compared to net income of ... for the second quarter of 2009 was $0.01, compared to basic net income ...
... , , , , ... ABT ) today announced the expansion of the company,s XIENCE ... of the company,s market-leading XIENCE V(R) Everolimus Eluting Coronary Stent System ... allows for more than 2,000 patients from the XIENCE V USA ...
... 13 /PRNewswire-FirstCall/ - Spectral Diagnostics Inc., (TSX: SDI), a ... a therapeutic targeting severe sepsis, today announced its financial ... , "During the quarter, results of the EUPHAS trial ... Medical Association (JAMA). The findings showed that the Toraymyxin(TM) ...
Cached Biology Technology:Cadus Reports Second Quarter 2009 Results 2Cadus Reports Second Quarter 2009 Results 3Cadus Reports Second Quarter 2009 Results 4Cadus Reports Second Quarter 2009 Results 5Abbott Expands XIENCE V(R) USA Trial to Enroll Patients into Landmark Dual Anti-Platelet Therapy (DAPT) Trial 2Abbott Expands XIENCE V(R) USA Trial to Enroll Patients into Landmark Dual Anti-Platelet Therapy (DAPT) Trial 3Abbott Expands XIENCE V(R) USA Trial to Enroll Patients into Landmark Dual Anti-Platelet Therapy (DAPT) Trial 4Spectral announces second quarter 2009 results 2Spectral announces second quarter 2009 results 3Spectral announces second quarter 2009 results 4Spectral announces second quarter 2009 results 5Spectral announces second quarter 2009 results 6Spectral announces second quarter 2009 results 7Spectral announces second quarter 2009 results 8
(Date:9/2/2014)... take stress in stride; others are done in by ... molecular mechanisms of this so-called stress gap in mice ... could lead researchers to better understand the development of ... people, each animal has unique experiences as it goes ... experiences can alter the expression of genes, and as ...
(Date:9/1/2014)... the U.S. has improved steadily in recent yearsspurred in ... quality remains poor and disparities continue to widen among ... from Harvard School of Public Health (HSPH). , "The ... the extensive efforts by many groups and individuals to ... it also indicates that these efforts need to be ...
(Date:9/1/2014)... Conventional wisdom has long held that corals ... reefs are passive organisms that rely entirely ... as nutrients and oxygen. But now scientists at ... in Israel have found that they are far ... into turbulent patterns that greatly enhance their ability ...
Breaking Biology News(10 mins):Discovery hints at why stress is more devastating for some 2Discovery hints at why stress is more devastating for some 3Quality of US diet shows modest improvement, but overall remains poor 2Quality of US diet shows modest improvement, but overall remains poor 3Nature's tiny engineers 2Nature's tiny engineers 3
... May 12, 2010 In findings that should finally ... of neurobiology, a team at The Scripps Research Institute has ... active in a brain region known to play a key ... formation. The new study also shows that this system can ...
... reporting success in a first attempt to silence the ... forces and cause infections. In a study in ACS, ... specially designed plastic-like materials to soak up the substances ... messages. Elena Piletska and colleagues point out ...
... flight May 14 carrying three University of Colorado at ... understand how and why slimy and troublesome clumps of ... The experiments on biofilms -- clusters of ... surfaces -- are of high interest to space scientists ...
Cached Biology News:Scripps Research study overturns decade-old findings in neurobiology 2Scripps Research study overturns decade-old findings in neurobiology 33 payloads built by CU-Boulder set for launch on space shuttle Atlantis 23 payloads built by CU-Boulder set for launch on space shuttle Atlantis 3