Navigation Links
Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf ,,, cMaster RTplusPCR System

the tubes in which the poly-A-RNA had been fixed. The RNA template is therefore missing in the protocols.

Three different primer pairs were used:

Serotype G: Product app. 800 bp
Serotype D: Product app. 500 bp
Control primer of non-transcribed genomic region: Product app. 400 bp
Amplification reactions were run as duplicates.

One-step RT-PCR

Preparation

Components Volume Final concentration in the reaction dNTP mix (each 10 mM) 1.0 l 200 M Forward primer G (100 M) 1.0 l 2 M Reverse primer D (100 M) 1.0 l 2 M RTplusPCR buffer with 25 mM Mg++ 5.0 l 1 x RNase inhibitor (0.5 g/l) 0.5 l 5 ng/l cMaster RT enzyme (15 U/l) 0.5 l 0.15 U/l cMaster PCR enzyme mix (5 U/l) 0.5 l 0.05 U/l RNase-free water 40.5 l Total volume 50.0 l


Program parameters of the one-step RT-PCR

Step Temp. Duration cDNA synthesis 50C 50 min Initial denaturation 94C 2 min Denaturation 94C 20 s
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. RT-PCR Primers for the Study of Apoptosis
8. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
(Date:5/21/2015)... /PRNewswire/ - RepliCel Life Sciences Inc. (TSX.V: RP) ... focused on the development of autologous cell therapies, ... International Society for Cellular Therapy (ISCT) on RepliCel,s ... in a Phase 1/2 clinical trial.  The presentation, ... 5:30 PM to 7:00 PM local time, will ...
(Date:5/20/2015)... MA (PRWEB) May 20, 2015 This ... history, especially for lawns. US Patented Pearl’s Premium ... for lawns around the country that are coming out ... that addresses a number of major global concerns related ... the “non-grass” grass – the alternative to the standard, ...
(Date:5/20/2015)... Westport, CT (PRWEB) May 20, 2015 ... the 10th anniversary of its acquisition of Tiger-Sul. ... from Enersul (Calgary Alberta, Canada) in May 2005, H.J. ... line to build it into a comprehensive portfolio of ... the strength of H.J. Baker and the innovation of ...
(Date:5/20/2015)... , May 19, 2015 Research and ... the addition of the "Bone Morphogenetic Proteins ... Fusion, Trauma, Reconstructive and Oral - Maxillofacial) - ... Forecast 2014 - 2022" report to their ... market is studied based on the types of ...
Breaking Biology Technology:RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 2RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 3Breakthrough 4th Generation Low Maintenance Lawn Seed Offers Solution to California Drought 2Agricultural Firm H.J. Baker & Bro., Inc., Celebrates 10 Years Since Acquisition Of Tiger-Sul 2Agricultural Firm H.J. Baker & Bro., Inc., Celebrates 10 Years Since Acquisition Of Tiger-Sul 3Global Bone Morphogenetic Proteins Market Industry Analysis 2014 - 2022 2
... student teams with high-tech diagnostic and surgical devices ... annual BMEidea awards at MD&M East Conference in ... June 10 Today three collegiate biomedical engineering ... The winners in the annual Biomedical Engineering Innovation, ...
... ... gene synthesis has long been misunderstood. DNA2.0 has cracked the code on ... technology. DNA2.0,s approach goes beyond codon arrangement, so the company is introducing ... patent has been proven to increase protein expression yields by up to ...
... Ongoing , , DALLAS, June 10 ... (OTC Bulletin Board: ACCP) , announced today ... Patent and Trademark Office that two US patents relating to ... the management of mucositis will issue in June as US Patent ...
Cached Biology Technology:NCIIA Honors Top Collegiate Biomedical Engineering Teams 2NCIIA Honors Top Collegiate Biomedical Engineering Teams 3DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches 2DNA2.0 Awarded US Patents for Breakthrough Gene Optimization Technology -- Company's Unique Algorithm Enables Expression Yields up to 50 Times Greater than Competing Approaches 3Access Pharmaceuticals Announces Granting of Two US Patents for MuGard(TM) 2Access Pharmaceuticals Announces Granting of Two US Patents for MuGard(TM) 3
(Date:5/8/2015)... JOSE, Calif. , May 8, 2015 Synaptics ... interface solutions, today announced that members of the executive management ... J.P. Morgan 43rd Annual Technology, Media and Telecom Conference ... Waterfront Hotel in Boston, MA ... May 27, 2015 Time: 2:45pm ET Location: The New York ...
(Date:4/27/2015)... , April 27, 2015  For more than ... trade show has been the premier worldwide event for ... 2015 is a must-attend event for any local, national, ... into the emerging commercial markets and current applications of ... innovations of this projected $48 billion industry, and how ...
(Date:4/20/2015)... PASADENA, Calif. , April 20, 2015 ... San Gabriel Valley to implant a new miniaturized, wireless ... HF System is the first and only FDA-approved heart ... reduce hospital admissions when used by physicians to manage ... a sensor that is implanted in the pulmonary artery ...
Breaking Biology News(10 mins):Synaptics to Present at Upcoming Investor Conferences 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3
... School in Plymouth, UK, have carried out research that suggests ... children from health experts may not be enough to tackle ... been published in the most recent issue of the journal ... the EarlyBird study, which has followed the development of over ...
... Austrian and Swiss scientists conducted intensive studies of the ... mountains and valleys. Around the same time, the Swiss ... at locations above 5000 metres, studying and photographing the ... black and white photographs taken by these scientists are ...
... be the focus of the Society for Nutrition Education,s ... one small step in fighting world hunger. Food security ... immediate attention in order to combat the issues of ... Nutrition Education (SNE) encourages its members and the public ...
Cached Biology News:Recommendations for children's exercise lacking say experts 2'Himalaya -- Changing Landscapes' photo exhibition draws attention to the impacts of climate change 2'Himalaya -- Changing Landscapes' photo exhibition draws attention to the impacts of climate change 3World Food Day brings attention to food security around the globe 2
... ICE-LAP3 Mch3 Lyophilized solid. ... enzyme regulation and kinetics, cleavage of target substrates, and ... protein. One unit is defined as the ... Ac-DEVD-pNA Cat. No. 235400 per hour at 37 C ...
... Cytokeratin 5 / 6 Cytokeratin 5 ... basic type of cytokeratin expressed in basal, ... of stratified epithelia as well as transitional ... and mesothelioma. Cytokeratin 6 (56 kD) is ...
Bovine Coronary Artery Smooth Muscle Cells (BCASMC) (>500,000 cells)...
RECK (F-20)...
Biology Products: