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Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS

e obtained from this instrument. Usually MS3 is sufficient to establish the fragmentation cascade because all fragments are generated abundantly without lower mass information cut off comparing with 3D ion traps.

Chloramphenicol eluted from the LC at t = 4.31 minutes. During the runs, one major metabolite -glucuronide of CAP was identified as well as CAP itself. This metabolite was well confirmed with both single scan and product of 497 (321 parent + 176 Glucuronic acid portion) scan in one chromatographic run along with quantitation using MRM mode. The metabolism result is in a good agreement with USP in vivo data. The quadrupole-linear ion trap proved to be a sensitive and reliable HPLC detection system that provided important chemical structure and quantitation information simultaneously.

References

1. J. Assoc. Off. Anal. Chem. 61, 1247 (1978). 2. Center for Veterinary Medicine (2001), Guidance for Industry: Mass Spectrometry for Confirmation of the Identity of Animal Drug Residues. 3. Al Pfenning, Sherri Turnipseed, Jose Roybal, Cathy Burns, Mark Madson, Joseph Storey, Rebecca Lee, U.S. Food And Drug Administration Laboratory Information Bulletin No. 4289, August 2002, Confirmation of Chloramphenicol Residues In Crawfish by Electrospray LC/MS.


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