Navigation Links
Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS

r that the peak one is CAP-glucuronide, and peak two is the parent CAP in Figure 1. Single mass spectrum of CAP shows [M-H]- pair m/z 321 and 323 (Figure 4). Product ion spectrum (Figure 5) shows two predominant ions m/z 121 and 152, which correspond to [C6H4NO2-H]- and [C6H4NO2+CH2O]-. Ions m/z 194 [m/z 321-C2H2NOCl2]-, 176 [m/z 194-(H2O)]-, 219 [m/z 321-(HCOCl2)-2H]-, 249 [m/z 321-HCl2]-, and 257/259 [m/z 321-(HCOCl)]- also present in the spectrum (Figure 5). The product ion spectrum shows the isotopic ion pair of m/z 257 and 259, which gives approximately equal abundance, indicating the loss of one chlorine atom (either 35Cl or 37Cl) from the 35Cl 37Cl parent ion. The single MS spectrum (Figure 2.) of CAP metabolite and its product ion spectrum (Figure 3.) stated that the metabolite is glucuronidation of CAP. Figure 4. Single MS spectrum of peak 2 at t = 4.31 minutes from Figure 1. Figure 5. Product ion spectrum of peak 2 (M/z 323) at t = 4.31 minutes from Figure 1. Figure 6. MRM quantitation chromatogram of the same analysis run shown in Figure 1. Quantitative Confirmation: The MRM mode quantitation of CAP has been obtained within the same run with the qualitative information collections. The results show in Table 1 with an example chromatogram (t=60 minutes) shows in Figure 6. It is a fast run (CAP elution time t = 4.31minutes). T1/2 was calculated to be 46 minutes and its plot shows in Figure 7.

Conclusions

As stated previously, the advantages for this method are fast run time (about 5 minutes for a entire run) and simultaneously doing qualitative and quantitative work together. The instrument is based on a quadrupole ion path and is capable of doing conventional multiple reaction monitoring (MRM) mode quantitation as a tandem mass spectrometer. On top of that it also has several high sensitivity ion trap MS scans using the final quadrupole as a linear ion trap. MS3 spectrum can b
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of nitrofurane metabolites in food with HPLC/MS/MS
2. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
3. Outstanding transfection rates using the Multiporator
4. Outstanding transfection rates using the Multiporator
5. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
6. Normalizing cDNA libraries using the EppendorfThermomixer
7. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
10. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
11. Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
Post Your Comments:
(Date:4/27/2015)... 27, 2015 SynGen Inc., a company that ... that harvest stem and progenitor cells from umbilical cord ... today that it has added three new members to ... Blood Business Units focus on product commercialization. ... its Vice President and Chief Financial Officer.  Chuck was ...
(Date:4/27/2015)... tru Shrimp™ Systems announced today that it has ... the exclusive worldwide rights to commercialize Tidal Basin™ technology, ... agreement for the patented technology, developed by world-renowned shrimp ... China, Taiwan, France, Germany, Italy, Spain, the Netherlands and ... from all over the world for our Tidal Basin™ ...
(Date:4/24/2015)... April 24, 2015 Top student social ... San Diego area universities, including UCSD and SDSU, will ... from prominent local, national and nonprofit companies for an ... 5th Annual Social Innovation Challenge 2015 . , ... social venture ideas addressing a diverse range of issues ...
(Date:4/24/2015)... DIEGO , April 24, 2015  Pfenex Inc. ... the development of biosimilar therapeutics including high value and ... its follow-on public offering of 6,000,000 shares of its ... $15.50 per share. Of the shares being offered, 2,610,000 ... shares are being offered by existing stockholders. Pfenex will ...
Breaking Biology Technology:SynGen Inc. Expands Its Management Team 2tru Shrimp™ Systems Acquires Global Patent for Tidal Basin™ Shrimp Production 25th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 25th Annual Social Innovation Challenge at University of San Diego - Students Pitch to Live Judging Panel to Take Home $75K 3Pfenex Inc. Announces Pricing Of Follow-on Offering 2
... LA JOLLA, Calif., March 18 TorreyPines Therapeutics,Inc. ... initiated a Phase II study of,NGX267, a muscarinic ... to Sjogren,s syndrome., The company is conducting ... square design to evaluate,the safety, tolerability and efficacy ...
... NEOG ) announces the following Webcast:, What: Neogen ... EDT, Where: http://www.videonewswire.com/event.asp?id=46587 , How: ... the address above., ... are unable to participate during the live webcast, the call will,be archived ...
... - Oncolytics Biotech Inc.,(TSX: ONC, NASDAQ: ONCY) ("Oncolytics") ... 7,344,711 entitled "Use of Adenoviruses Mutated in the,VA ... of treating,cancer using adenoviruses that are modified to ... Ras pathway., "This patent expands our adenovirus ...
Cached Biology Technology:TorreyPines Therapeutics Initiates Phase II Study of Muscarinic Agonist NGX267 for Treatment of Xerostomia 2TorreyPines Therapeutics Initiates Phase II Study of Muscarinic Agonist NGX267 for Treatment of Xerostomia 3TorreyPines Therapeutics Initiates Phase II Study of Muscarinic Agonist NGX267 for Treatment of Xerostomia 4Oncolytics Biotech Inc. Announces Issuance of 26th U.S. Patent 2
(Date:4/14/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market, announces that its ... New York Design Awards under the category  ,Product Design ... Design Awards are part of a global multi-disciplinary awards ... from the marketplace, industry and judging panel. Winners will ...
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... announced today that it will report financial results for ... 23, 2015, after the close of market. The company ... investors at 2:00 p.m. PT (5:00 p.m. ET), during ... To participate on the live call, analysts and investors ...
(Date:4/2/2015)... Md. , April 2, 2015 At ... Salt Lake City , the American College ... of five new directors to its Board.  Members of ... the ACMG and for forming and advancing its policies ... medical genetics profession. "It,s an eventful time ...
Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3Synaptics to Report Third Quarter Results on April 23 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8
... LEDs may help reduce skin ... in Germany are describing a potential alternative to Botox ... scheduled for the November 5 issue of ACS, ... that high intensity visible light from light emitting diodes ...
... cleavage product, amyloid-b (Ab), builds up into fibrous plaques ... one specialized membrane microdomain to another to be cleaved, ... definitive evidence that Ab plaques are the direct cause ... support this. And working on this hypothesis, scientists are ...
... one step closer to finding a vaccine that better ... at Saint Louis University appears likely to offer significantly ... one in current use. "Not only was it ... better immune response, which suggests it will be more ...
Cached Biology News:American Chemical Society Weekly PressPac Oct. 15, 2008 2American Chemical Society Weekly PressPac Oct. 15, 2008 3American Chemical Society Weekly PressPac Oct. 15, 2008 4American Chemical Society Weekly PressPac Oct. 15, 2008 5American Chemical Society Weekly PressPac Oct. 15, 2008 6American Chemical Society Weekly PressPac Oct. 15, 2008 7American Chemical Society Weekly PressPac Oct. 15, 2008 8Research identifies type of vaccine that holds promise in protecting against TB 2
... on over 20 years of experience in ... Life Sciences, the Freedom EVO platform delivers ... according to future needs. Together with the ... is equipped to successfully operate genomics, proteomics, ...
... Use in any standard fixed-angle ... 16,000 g.,Spin- centrifuge tube filters ... within a centrifuge tube. They filter ... removal, HPLC sample preparation, removal of ...
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. Material: polystyrene ...
12 breakable polypropylene strips of 8 wells each held in a rigid frame. Ideal for antibody assays....
Biology Products: