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Simultaneous Determination of Residues of Approximately 100 Pesticides ,,, and Metabolites in Fruit and Vegetables by LC/MS/MS

cephate, carbofuran, atrazine, desmedipham, triasulfuron, cyprodinil and flufenoxuron). The criteria of selection of individual pesticides for this tuning mixture were the necessary Declustering Potential (DP) and the Collision Energy (CE). The aim was to take into account the whole range of possible parameter settings. The results of this instrument comparison are summarised in Table 1 (see over page). All parameters optimized for an API 2000 system can be directly used for the API 3000 and API 4000 systems or can be obtained with the help of the test mixture containing 8 compounds only. One parameter (DP) has to be shifted by a constant voltage and two parameters are fixed on API 3000 and API 4000 systems. Consequently, the transformation of optimum conditions from one instrument type to another is possible. At least a satisfactory sensitivity for the pesticides covered by the method will be obtained without individual optimizations of compound specific parameters. Eventually, if distinct pesticides show an unexpected low response, for these compounds only a specific tuning may be needed.

Content of the CD ROM

To assist users of Applied Biosystems/ MDS SCIEX mass spectrometers the CD ROM of this application note contains several files, which work with Analyst software version 1.2 or higher.

Method files (.dam): Method files for API 2000, API 3000, API 4000 LC/MS/MS Systems equipped with Agilent 1100 binary pump, degasser, autosampler, column oven and optional with UV detector, and for API 2000, API 3000, API 4000 LC/MS/MS Systems equipped with Agilent 1100 binary pump, degasser, column oven and HTC/CTC autosampler (PAL) plus optional UV detector are given.


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