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Simultaneous Determination of Residues of Approximately 100 Pesticides ,,, and Metabolites in Fruit and Vegetables by LC/MS/MS

cyprodinil, daminozid, demeton-S-methyl, demeton-S-methylsulfone, desmedipham, desmethylformamidopirimicarb, desmethylpirimicarb, dimethoate, diuron, ethiofencarb, ethiofencarbsulfone, ethiofencarbsulfoxide, fenhexamid, fenoxycarb, fenpropimorph, flazasulfuron, florasulam, fluazifoppbutyl, flufenoxuron, fosthiazate, furathiocarb, haloxyfopethoxyethyl, haloxyfopethyl, imazalil, imidacloprid, indoxacarb, iodosulfuronmethyl, iprovalicarb, isoproturon, linuron, metalaxyl, metamitron, methamidophos, methiocarb, methomyl, metolachlor, metsulfuronmethyl, monocrotophos, nicosulfuron, omethoate, oxamyl, oxydemetonmethyl, phenmedipham, pirimicarb, primisulfuronmethyl, promecarb, propamocarb, propoxur, prosulfuron, pymetrozin, pyridate, pyridatmetabolite, pyrimethanil, quinmerac, quizalofopethyl, rimsulfuron, spiroxamine, tebuconazole, tebufenozid, thiabendazol, thiacloprid, thifensulfuronmethyl, thiodicarb, thiofanox, thiofanoxsulfone,

thiofanoxsulfoxide, thiophanatmethyl, triasulfuron, tribenuronmethyl, triflusulfuronmethyl and vamidothion (Figure 2). Liquid chromatography was carried out using a system equipped with a binary pump, degasser and autosampler. For the separation an analytical column Aqua 5 125 A C18 (Phenomenex, Aschaffenburg, Germany) with the dimensions 50 mm x 2 mm was used. The mobile phase A consisted of 80% water and 20% methanol, mobile phase B of 90% methanol and 10% water. Both phases A and B contained 5 mmoL/L ammonium formiate. The flow rate was 0.2mL/min. The mobile phase composition was changed during a run as follows: Starting with 0%, the percentage of B was increased linearly to 100% over 11 min and then kept constant for another 12 min. Equilibration time prior to the next
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... Mch3 Lyophilized solid. Highly active ... kinetics, cleavage of target substrates, and inhibitor screening. ... One unit is defined as the amount of enzyme ... 235400 per hour at 37 C at saturated substrate ...
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