Navigation Links
Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method

of quantitation for each of these metabolites was determined to be: 1.0, 0.5 and 0.5 pg, respectively.

Calibration curves for the three metabolites were run in triplicate over the range of 1000 to 1 pg and are shown in Figures 3 - 5. The original xenobiotics can also be quantified, if desired, to determine percent usage of the drug (data not shown). Excellent linearity is seen within this range with the average correlation coefficient being 0.993. Accuracy in retention times of the metabolites is also excellent with an average 1.15% RSD, the dextrorphan being slightly more variable (2.74% RSD).

Conclusion

With this application note we have demonstrated a single method for the separation and quantification of the enzymatic activities of the three most important Cytochrome P450 enzymes (3A4, 2D6 and 2C9). This method can be used for pre-clinical evaluation of NCEs as inhibitors of CYPs or quantification of these metabolites from clinical samples after SPE.

References

1. Wrighton, S. A., Stevens, J. C. Crit. Rev. Toxicol. 1, 1-21 (1992).

2. Kwak, M. K. et al. Mutat. Res. 480-481, 305-15 (2001).

3. Honig, P. K. et al. J. Am. Med. Assoc. 269, 1513- 1518 (1993).

4. Kuntzman, R., et al. J. Pharmacol. Exp. Ther. 152, 151 (1996).

5. Lee, C.R., Goldstein, J.A., Pieper, J.A. Pharmacogenetics 3, 251-63 (2002).

6. Wojnowski, L. Ther. Drug. Monit. 26, 192-9 (2004).

7. Brockmoller J., et al. Pharmacogenomics 1, 125-151 (2000).


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
2. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
3. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
4. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
5. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
6. A New PCR-based Mycoplasma Detection Method
7. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
8. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
9. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
10. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
11. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
Post Your Comments:
(Date:8/26/2015)... /PRNewswire/ - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ... the quarter ended June 30, 2015. Amounts, unless ... presented under International Financial Reporting Standards ("IFRS"). ... our Oral Amp B program, rapidly advancing towards ... Andrew Rae , President & CEO of iCo ...
(Date:8/26/2015)... 26, 2015 Intrexon Corporation (NYSE: XON ... closing of its previously announced public offering of common ... of their option to purchase an additional 731,707 shares ... $41.00 per share.  The exercise of the underwriters, option ... sold by Intrexon to 5,609,756 shares and increased the ...
(Date:8/26/2015)... San Bruno, CA. (PRWEB) , ... August 26, 2015 , ... ... three major conferences to develop new business relationships with local sponsors in southern California. ... services to facilitate their drug development processes and regulatory pathways., , ...
(Date:8/25/2015)... Aug. 25, 2015 Human Longevity, Inc. (HLI), the ... has joined the company as Chief Operating Officer. Winham, ... operations and technical experience, will report directly to HLI,s CEO, ... this new role as COO, Winham will be responsible for ... well as all facility operations. HLI currently has three locations ...
Breaking Biology Technology:iCo Therapeutics Announces Second Quarter 2015 Financial Results 2iCo Therapeutics Announces Second Quarter 2015 Financial Results 3Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 2Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 3Intrexon Announces Closing of $230 Million Public Offering of Common Stock including Full Exercise of Underwriters' Option to Purchase Additional Shares 4PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 2PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 3Human Longevity, Inc. Hires Life Sciences Leader, Mark Winham, as Chief Operating Officer 2
... VIRGINIA BEACH, Va., Oct. 7, 2008 AMERIGROUP,Corporation ... will report its earnings,for the third quarter ended ... October 22, 2008. At 8:30 a.m. Eastern Time ... a conference call and webcast,to discuss earnings and ...
... of Lives, GERMANTOWN, Md., Oct. 7 ... development of medical,countermeasures for biodefense and emerging infectious ... for advanced development of a,vaccine to protect against ... Allergy and Infectious Diseases (NIAID), part of the ...
... Maryland, October 7 Orthocrat Ltd., announced,today ... purchased,TraumaCad(TM), a web-based pre-operative planning and digital ... used X-ray films and acetate,overlays to determine ... for,joint-replacement surgery. With the adoption of digital ...
Cached Biology Technology:AMERIGROUP Corporation to Discuss Third Quarter Earnings on October 23 2Integrated BioTherapeutics Awarded NIAID Contract For Up to $65 Million for Development of a Vaccine against Ebola and Marburg Viruses 2Integrated BioTherapeutics Awarded NIAID Contract For Up to $65 Million for Development of a Vaccine against Ebola and Marburg Viruses 3George Washington University Hospital Purchases Orthocrat's TraumaCad(TM) for Orthopedic Preoperative Planning 2George Washington University Hospital Purchases Orthocrat's TraumaCad(TM) for Orthopedic Preoperative Planning 3
(Date:8/20/2015)... Aug. 20, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market and ... joined The Smart Card Alliance and the EMV Migration ... and the Smart Card Alliance Latin America (SCALA) Chapter ... peers and thought leaders promoting adoption of smart card ...
(Date:8/18/2015)... Aug. 18, 2015  Navitas today announced ... to the Submission Content Management webinar scheduled ... non-profit forum. Dedicated to the Implementation of Regulatory ... platform for industry, vendors, health authorities, consultants ... work towards a standard method of implementing ...
(Date:8/17/2015)... OXFORD, Conn. , Aug. 17, 2015  NXT-ID, ... a biometric authentication company focused on the growing mobile ... announces its new sales campaign for Q4 2015. The ... winning marketing team consisting of Amplitude Marketing Group ( ... Amplitude Marketing Group is a leader in retail driven ...
Breaking Biology News(10 mins):NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4Navitas, a Key Contributor in IRISS Forum Webinar 2Navitas, a Key Contributor in IRISS Forum Webinar 3NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3
... of the greatest mysteries of modern coral reefs is how ... has long existed in the record of coral evolution. ... in sea level and changes in the Earth,s climate between ... many "old" corals went extinct, and the modern reef corals ...
... the University of Alberta have made a discovery that may ... cow disease, adapt in order to spread between various types ... discovered that a miniscule change in the prions, makeup appears ... mimic and recreate new strains with which it comes into ...
... For hundreds of years, naturalists and scientists have ... differences. But now, new genetic techniques are revealing ... visible differences. In a just-published study, evolutionary ... the American Museum of Natural History (AMNH) combine ...
Cached Biology News:University of Miami geologists to address the mystery of an evolution gap in reef corals 2Discovery may explain how prion diseases spread between different types of animals 2Researchers develop tools for discovering new species 2Researchers develop tools for discovering new species 3
...
... pAcGHLT-A,B,C Baculovirus ... Set,pAcGHLT-XylE Baculovirus ... Powder,Thrombin Dilution ... Cocktail,Insect Cell ...
...
... Plus accurately and conveniently controls the temperature at ... different temperature levels up to 99 C*, with ... as low as 5 C. The thermoblocks for ... tubes, and microplates enable a high degree of ...
Biology Products: