Navigation Links
Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method

uis, MO) with the exception of midazolam and α-hydroxymidazolam standard solutions (1 mg/mL and 100 μg/mL in methanol, respectively) from Cerilliant (Round Rock, TX).

Sample Preparation

Stock solutions were prepared in HPLC grade water (dextrorphan) or methanol (4-hydroxytolbutamide) at 1 mg/ mL. All diluted samples were prepared in 50:50 methanol: water (HPLC grade).

Discussion

Testing of drug metabolizing enzymes (such as cytochrome P450s) is becoming increasingly important for the evaluation of new chemical entities (NCEs) before evaluation of the new drug in the clinic. Newly synthesized compounds that display proper biological effects on the target of choice in vitro, can and should be evaluated for their effects on drug metabolizing enzymes before an expensive scale-up of the new drug takes place for clinical testing. CYP3A4, 2D6 and 2C9 were chosen for our evaluation because 45-60% of the drugs currently on todays market are metabolized by CYP3A isoforms alone6, and CYP2D6 and CYP2C family members are subject to polymorphisms leading to populations of people that are poor metabolizers7. Having a combined method for all three would, therefore, be useful for testing samples from all of these enzymes at one time, eliminating the need to switch solvents and/or whole methods to evaluate these enzymes.

Good separation of the metabolites is obtained, in less than 5 minutes, on a Varian MonoChrom MS column with 0.1% formic acid (Solvent A) and acetonitrile (Solvent B) with the gradient mentioned (see LC Method, above). Figure 2A C, shows the individual peaks as detected by single reaction mode (SRM) in the 1200L. Limit of detection (LOD) for each of these metabolites was determined to be: 0.5, 0.25 and 0.25 pg (4-hydroxytolbutamide, -hydroxymidazolam and dextrorphan, respectively) and the limit
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
2. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
3. Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS
4. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
5. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
6. A New PCR-based Mycoplasma Detection Method
7. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
8. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
9. Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
10. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
11. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
Post Your Comments:
(Date:1/23/2015)... (PRWEB) January 23, 2015 Portal Solutions ... digital workplaces on Microsoft Office 365 and SharePoint platforms ... 50 Award program for being one of the region’s fastest-growing ... and employee growth. , “To be recognized a second ...
(Date:1/22/2015)... 2015 Shimadzu Scientific Instruments today ... UV-visible spectrophotometer. Offering wavelength scanning from 190 to ... for applications in a variety of industries, including ... user-friendly UV-1280 enables intuitive operation, while the enhanced ...
(Date:1/22/2015)... NH (PRWEB) January 22, 2015 GEA ... a table top laboratory homogenizer, the PandaPLUS 2000, which ... nanodispersions, nanoemulsions , and cell disruption . This ... dairy products, fruit juices, liquid food, food additives and ...
(Date:1/22/2015)... Cambridge Semantics, the leading provider of smart data ... was a record-breaking year across the board for the company. ... Data Platform and our Smart Data solutions, our customers were ... which led to record growth for the company in 2014,” ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2Cambridge Semantics Announces Record Results for 2014 2
... That HuMax-Inflam Target is IL-8 and Future ... Development Will be in Cancer and Inflammation, ... its fully human HuMax-Inflam(TM) antibody is directed,to IL-8 (interleukin-8) ... will initially focus on studies to treat glioblastoma,a cancer ...
... N.C., Sept. 12 PPD, Inc. (Nasdaq:,PPDI) today ... financial,officer. He will assume his position on October ... after 25 years with Honeywell,International, a $34 billion ... served as vice president and CFO of finance,transformation ...
... DIEGO, Sept. 12 Gen-Probe Incorporated,(Nasdaq: GPRO ) ... of,America,s 37th Annual Investment Conference in San Francisco on ... p.m. Eastern Time).,The presentation is scheduled to be webcast ... investor information section of Gen-Probe,s website,at http://www.gen-probe.com . ...
Cached Biology Technology:Genmab Discloses Target and Development Plans for HuMax-Inflam 2Genmab Discloses Target and Development Plans for HuMax-Inflam 3PPD Names Daniel Darazsdi as Chief Financial Officer 2PPD Names Daniel Darazsdi as Chief Financial Officer 3Gen-Probe to Webcast Presentation at Bank of America's 37th Annual Investment Conference 2
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ... "iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & ... http://photos.prnewswire.com/prnh/20130307/600769 ... introduced the fingerprint reading feature with the iPhone 5S. ...
(Date:12/17/2014)... FORT BELVOIR, Va. , Dec. 15, 2014 ... make it easier to detect and prevent counterfeit microcircuits ... the agency started performing an in-house microcircuit anti-counterfeit initiative ... of purchased microcircuits while increasing their reliability throughout the ...
(Date:12/15/2014)... , Dec. 12, 2014 Research and Markets ... "Global Facial Recognition Market 2015-2019" report to ... Facial recognition is a technology used for ... overall facial feature of a person such as nose, ...
Breaking Biology News(10 mins):iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3
... Los Baos, Philippines The International Rice Research Institute ... scientific advances and address some of the biggest unsolved ... and distribute improved varieties of rice that can be ... access to irrigationand withstand environmental stresses such as drought, ...
... This release is available in German . ... Biology by researchers of the Robert Koch Institute (Berlin), ... the Centre Suisse des Recherches Scientifiques (Ivory Coast) confirms ... virus transmission from humans to wild apes. The study ...
... January 2008) Determined to revive the grossly depleted ... cause of poverty and hunger, the Alliance for a ... million five-year program to restore the fertility of Africas ... life into soils where rapid nutrient loss is sapping ...
Cached Biology News:Gates funding to help poor rice farmers 2Gates funding to help poor rice farmers 3Gates funding to help poor rice farmers 4Great apes endangered by human viruses 2Alliance for a Green Revolution in Africa commits 180M to revive farmers' depleted soils 2Alliance for a Green Revolution in Africa commits 180M to revive farmers' depleted soils 3Alliance for a Green Revolution in Africa commits 180M to revive farmers' depleted soils 4
Plasmid expressing the LacZ reporter gene....
Human SEAP ORF, provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
... PYREX Fernbach-style culture flasks have triple baffles ... bottom to achieve maximal oxygen transfer to ... marking spot., A wide range of optional ... Polypropylene colored caps for ease of ...
... 400g of purified RNA samples., , This ... of aqueous RNA samples that have been ... phenol/chloroform, silica, oligo dT etc or other ... the necessary components for the stabilization of ...
Biology Products: