Navigation Links
Simple and Rapid Isolation of Plant RNA,,, Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

nt pellet to a fresh 50 ml tube without any carryover of the cell debris. If the pellet is loose, the supernatant should be filtered through an RNase-free Miracloth (Callbiochem).
  • Add 18 ml 70% ethanol to lysate and completely mix via very gentle, repeated inversion.
  • Add 4 ml well-mixed Perfect RNA Binding Matrix Solution and mix via gentle inversion.
  • Place two Perfect RNA Maxi Spin Columns into 50 ml centrifuge tubes and transfer lysate/Binding Matrix mixture to two Spin Column assemblies.
    Note: A modified preparation procedure for difficult tissues is described in the detailed protocol Step 6.
  • Centrifuge for 5 to 10 min. at 2,000 x g. Discard filtrate.
  • Add 10 ml Wash Solution I to each Spin Column and centrifuge for 5 min. at 2,000 x g. Carefully transfer Spin Columns to fresh centrifuge tubes without splashing Wash Solution I.
  • Add 10 ml Diluted Wash Solution II (ethanol not provided) to each Spin Column and centrifuge 5 min. at 2,000 x g. Carefully transfer dry Spin Columns to RNase-free 50 ml Collection Tubes (provided).
  • Add 1 ml Molecular Biology Grade Water to each Spin Column and vortex assemblies briefly.
  • Incubate at 50C for 5 min.
  • After incubation, vortex for 5 sec. and immediately centrifuge for 5 min. at 2,000 x g. Store recovered total RNA at 80C.
  • Results

    About 200 g RNA of each were obtained from 1 g source material of Arabidopsis leaves, flowers, or pods, while 5 g yielded about 800 g RNA, and 10 g yielded 850 g RNA. With respect to maize, the yields were somewhat lower: 180 g, 560 g, a
    '"/>

    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
    2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
    3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
    4. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
    5. Simple Isolation of RNA from Tissue and Cultured Cells
    6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
    7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
    8. Simple Approaches for Optimization of RT-PCR
    9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
    10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
    11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
    Post Your Comments:
    (Date:10/30/2014)... SoundConnect , an industry leading ... end users the key to turning this already ... adding voice enablement and hybrid audio conferencing. , ... communication tool that includes presence, instant messaging and ... becomes a comprehensive unified communications solution. Adding the ...
    (Date:10/30/2014)... 30, 2014 Avure Technologies celebrates a year ... newest member of its fleet of high volume High Pressure ... anniversary as we return to PackExpo this year, which is ... Las Vegas,” said Jeff Williams, CEO at Avure. “Sales of ... pleased to be meeting the challenges of market demand for ...
    (Date:10/30/2014)... A solution to one of the key challenges in ... University of Sussex physicists. , In a paper published ... Barry Garraway and colleagues show how to make a ... , Quantum technology devices, such as high-precision sensors and ... interaction of atoms. But the methods for trapping these ...
    (Date:10/27/2014)... WORCESTER, Mass. and TORONTO ... Biotechnology Corporation ( www.generex.com ) (OTCQB: GNBT) today ... the immunological response triggered by its novel proprietary ... of relapse in breast cancer patients. The AE37 ... wholly-owned subsidiary, Antigen Express, Inc. ( www.antigenexpress.com ). ...
    Breaking Biology Technology:SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 2In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 3Generex Announces Presentations Correlating Immunological Response with Reduced Recurrence in Phase II Study of AE37 Breast Cancer Vaccine in Patients at the American College of Surgeons 2Generex Announces Presentations Correlating Immunological Response with Reduced Recurrence in Phase II Study of AE37 Breast Cancer Vaccine in Patients at the American College of Surgeons 3Generex Announces Presentations Correlating Immunological Response with Reduced Recurrence in Phase II Study of AE37 Breast Cancer Vaccine in Patients at the American College of Surgeons 4
    ... up a new Lake Superior chapter in Ashland in ... Wisconsin. , ,WIN organizes networking events, seminars and conferences. ... Council , a state-funded adviser to the governor and ... in Madison, Milwaukee, the Chippewa Valley and the Fox ...
    ... committee wrestles with balancing the state's biennial budget, calls ... Wisconsin System will likely move front and center. In ... one of the state's five biggest spending programs. , ... UW System programs that contribute to the state's economic ...
    ... fuel source for Wisconsin may be where consumers least expect ... Wisconsin Department of Administration announced on Monday a ... Madison Environmental Group consulting firm to develop cars operating ... restaurant chain Culver's , which has successfully modified two ...
    Cached Biology Technology:Academic R&D pays for itself in jobs and economic growth 2Academic R&D pays for itself in jobs and economic growth 3Environmental group works to run cars on Culver's old frying oil 2Environmental group works to run cars on Culver's old frying oil 3
    (Date:10/29/2014)... German . ... Schlögelhofer at the Max F. Perutz Laboratories (MFPL) of ... Vienna dived into the process of meiosis in specific ... inversion of the standard meiotic phases. The researchers describe ... Communications . , Meiosis is the two-step series of ...
    (Date:10/29/2014)... Hebei, China — BGI Tech and Hebei Agricultural University ... Jujube genome. Jujube is the most economically important member ... particularly difficult to sequence due the high level of ... time that a genome in the Rhamnaceae (Buckthorn) family ... in Nature Communications . , Jujube ...
    (Date:10/29/2014)... research by the international Cancer Genomics of the ... connection between kidney cancer and exposure to aristolochic ... findings, published in Nature Communications , have ... kills more than 140,000 people every year, and ... dramatically. CAGEKID, part of the International Cancer Genome ...
    Breaking Biology News(10 mins):Meiotic cell division 'the other way round' 2BGI Tech and Hebei Agricultural University complete the genome sequencing of the jujube tree 2Kidney cancer in Central Europe 2
    ... are the destination for RRS James Cook,s current expedition. ... hydrothermal vents, calderas and cold seeps on the seabed ... Paul Tyler of the University of Southampton Ocean and ... Centre, the scientists are investigating four sites that were ...
    ... CHICAGO -- Northwestern Medicine researchers have discovered a ... cancer, with opposing functions that can suppress or promote ... (Treg) cells, known to suppress immune responses in healthy ... presence of the protein RORγt has been shown to ...
    ... Orleans, LA Kathleen Sebelius, Secretary of the United States ... Weiss, MD, Professor and Chair of the Department of Ophthalmology, ... of LSU Eye Center of Excellence at LSU Health Sciences ... Council. Effective immediately, she will serve a four-year term. Dr. ...
    Cached Biology News:Finding life in the volcanic systems of the Antarctic Polar Front 2'Two-faced' cells discovered in colon cancer 2LSUHSC's Weiss chosen to help set national eye policy, research 2LSUHSC's Weiss chosen to help set national eye policy, research 3