Navigation Links
Simple and Rapid Isolation of Plant RNA,,, Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

Sonja Vorwerk and Barbro Patterson
Sonja Vorwerk, Max Planck Institut fr Zchtungsforschung, Carl-von-Linn Weg 10, 50829 Cologne, Germany
Phone: ++49-221-5062-441, Fax. ++49-221-5062-413, e-mail: vorwerk@mpiz-koeln.mpg.de
Barbro Patterson, Eppendorf AG, Germany Abstract

The isolation of plant RNA is a particularly challenging task. There are many protocols for the isolation of total RNA from plants that utilize solvents such as phenol or chloroform; however, their toxicity and the resulting cumbersome disposal may make alternative protocols more desirable. This application protocol demonstrates that the Eppendorf Perfect RNA Maxi Kit for eukaryotic cells is ideally suited for the isolation of total RNA from plant material, shown here with the sources Arabidopsis thaliana (dicotyledon) and Zea mays (monocotyledon).

The technology of the Perfect RNA Kit is based on cell lysis with a chaotropic guanidinium isothiocyanate solution, resulting in the rapid inactivation of cellular RNases. The RNA in the lysate is then selectively bound to a specially designed matrix and purified from DNA and other contaminants by efficient washing steps. Finally, RNA is eluted in molecular biology grade (RNase-free) water.

Materials and Methods

Arabidopsis plants, maize plants, and a maize cell culture were used for the experiments. Various amounts of starting material were used, from 110 mg (see results listed below). First, the plant material was ground very thoroughly to a fine powder in liq
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
5. Simple Isolation of RNA from Tissue and Cultured Cells
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Post Your Comments:
(Date:9/4/2015)... Francisco, CA (PRWEB) , ... September 04, 2015 ... ... the delivery of product safety and pharmacovigilance services and software tools to pharmaceutical ... application, PRIMO Mobile by Allergan Japan. PRIMO Mobile is a mobile data intake ...
(Date:9/3/2015)... Texas , Sept. 3, 2015  Vermillion, Inc. ... on gynecologic disease, today announced that Herbert Fritsche ... subsidiary, ASPiRA LABS, is the recipient of the 2015 ... and Biomarkers (ISOBM) for outstanding contributions to the field ... the award on October 5 th in Zakopane, ...
(Date:9/3/2015)... Santa Clara, CA (PRWEB) , ... September 03, 2015 , ... ... 3D printing and additive manufacturing on Sept. 17, 2015 at 9am PST. The webinar ... understanding and application. More popularly known as 3D printing, additive manufacturing is a broad ...
(Date:9/2/2015)... , Sept. 2, 2015 ... announced the addition of Jain PharmaBiotech,s new ... to their offering. ... pharmaceutical and biotechnology industries is anticipated. Nanotechnology ... drug development - from formulations for optimal ...
Breaking Biology Technology:November Research Group’s PRIMO Mobile Goes Live at Allergan Japan 2Vermillion Announces Dr. Herbert Fritsche as Recipient of the 2015 Abbott Award from the International Society of Oncology and Biomarkers 2Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 2Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 3Park Systems Announces Webinar on 3D Printing and Additive Manufacturing for Nano-Scientists, Researchers in Nanotechnology and Engineers 4Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 2Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 3
... ... grows, albeit more slowly than in 2009, changes to the pharmaceutical landscape could mean the ... ... of a confusing month in terms of the employment situation. Overall, the Bureau of Labor ...
... A cheap and simple way of making vaccines stable ... to work by scientists at Oxford University. The ... removes the need for fridges, freezers and associated health ... - particularly in the developing world where infectious diseases ...
... , MILFORD, Mass. ... Waters Corporation and DAISO CO., LTD. jointly ... agreement to globally market and supply DAISOGEL™ bulk packing ... both organizations will aid pharmaceutical and biopharmaceutical companies as ...
Cached Biology Technology:The MedZilla Report for February 2010 - Health Care Growth Slows; Big Pharma May Cut Thousands as Sales Landscape Changes 2The MedZilla Report for February 2010 - Health Care Growth Slows; Big Pharma May Cut Thousands as Sales Landscape Changes 3The MedZilla Report for February 2010 - Health Care Growth Slows; Big Pharma May Cut Thousands as Sales Landscape Changes 4New method makes vaccines stable at tropical temperatures 2New method makes vaccines stable at tropical temperatures 3Waters and DAISO Sign Marketing Agreement for Bulk Process Chromatography Media 2Waters and DAISO Sign Marketing Agreement for Bulk Process Chromatography Media 3
(Date:8/20/2015)... 20, 2015 The wearable technology market ... active and healthy. However, wearable technology has the ... to help improve diagnostic capabilities and therapeutic outcomes. ... Wearable Technologies Conference 2015 , where ... wearables in healthcare.    "Over the ...
(Date:8/20/2015)... CT ., Aug. 20, 2015 NXT-ID, ... a biometric authentication company focused on the growing mobile ... announces it has joined The Smart Card Alliance and ... Smart Card Alliance and the Smart Card Alliance Latin ... visibility to industry peers and thought leaders promoting adoption ...
(Date:8/18/2015)... , Aug. 18, 2015  Navitas ... key contributor to the Submission Content Management ... IRISS, a non-profit forum. Dedicated to the Implementation ... a neutral platform for industry, vendors, health ... information and work towards a standard method ...
Breaking Biology News(10 mins):Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4Navitas, a Key Contributor in IRISS Forum Webinar 2Navitas, a Key Contributor in IRISS Forum Webinar 3
... (Atlanta, Ga. August 20, 2008) Today, the U.S. Environmental ... grant to the University of Kentucky (UK) to investigate how ... enter the brain. This is the largest EPA Science ... of Kentucky as well as the largest single grant ever ...
... (Aug. 20, 2008) The genetic code of a simple saltwater ... function in humans and other species. A study published this ... code of Trichoplax , a simple saltwater creature that one ... aquarium. "We,re trying to identify, in the Trichoplax , ...
... heart attacks. But to do that they have to provide ... a team led by William Maisel at Harvard Medical School ... modify wireless communications from a pacemaker ( New Scientist, ... monitor and adjust the implanted device, but a malicious hacker ...
Cached Biology News:$2M grant awarded to University of Kentucky for research on nanoparticles and human health 2Genome of saltwater creature could aid understanding of gene grouping 2How to stop a new type of heart attack 2
... is a base medium for preparation of minimal ... sulfate but does not contain amino acids. Both ... Each pouch contains reagents to prepare 500 ml ... ml of 10X YNB for S. cerevisiae media. ...
... CUL-3 is a member of the family of human ... homologous to the S. cerevisiae cdc53 gene. Cullin-3 is ... It forms a tight complex with cyclin E and ... by protesomes. Cyclin E is an activator of cdk2, ...
Imject Freund's Incomplete Adjuvant (FIA)...
With sodium bicarbonate. Sterile, Cell culture tested...
Biology Products: