Navigation Links
Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

t was compared to PCR product purified using the silica-gel membrane method (figure 5). A 1.5-kb PCR product was generated using amplification primers, each containing a different restriction site sequence. The PCR product was digested with both restriction enzymes, purified using each method, ligated to digested pBluescript SK(+) vector and transformed into XL2-Blue ultracompetent cells. In five separate experiments, the 1.5-kb PCR product purified using the StrataPrep PCR purification kit and cloned showed an average transformation efficiency of 9.7 x 106 cfu/g of vector DNA. The percent of clones that contained inserts averaged 90%. For the 1.5-kb PCR product purified and cloned using the silica-gel membrane method, the transformation efficiency was 5.5 x 106 cfu/g of vector DNA, and the percent of clones that contained inserts averaged 82%. Thus, purification using the StrataPrep kit resulted in nearly a 2-fold increase in cloning efficiency and a higher percent of clones with inserts.

Gel Isolation

Figure 6

Figure 6 shows the yield of DNA fragments that were recovered from a conventional 1% agarose gel using either the StrataPrep DNA gel extraction kit or the silica-gel-membrane method. The two methods appear to be equivalent for fragments ranging in size from 0.25 to 9 kb. A higher yield of the 23-kb fragment is obtained with the StrataPrep DNA gel extraction kit. Transformation efficiencies for ligated 1.5- and 0.6-kb PCR products that were isolated with the StrataPrep DNA gel extraction kit were 1.7 and 9.8 x 106 cfu/g, respectively. These values were equival
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
4. Simple Isolation of RNA from Tissue and Cultured Cells
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:10/30/2014)... Spartan Bioscience announced today that Health Canada ... It detects CYP2C19 genetic mutations in less than 60 ... RX CYP2C19 System is the first near-patient DNA test ... Due to the system’s ease of use, Health Canada ... professionals such as doctors, nurses, pharmacists, and laboratory technicians, ...
(Date:10/30/2014)... Al Khaimah, UAE (PRWEB) October 30, 2014 ... appointment of Dr. James A. Manganello, Chairman of the Board, ... of The Bahamas. The appointment was made by the ... Bahamas on September 24th, 2014. , More than ... Therapy and Research Bill , Prime Minister Perry Christie said ...
(Date:10/30/2014)... 2014  Regado Biosciences, Inc. (Nasdaq: RGDO ... call and live audio webcast on Thursday, November 6, ... quarter 2014 financial results. Interested participants and ... 347-1165 for domestic callers or (412) 902-4276 for international ... the investor relations section of the Regado website at ...
(Date:10/27/2014)... Ryan Carfley , Managing Partner at ... with Bryan Hamilton, the publisher of the Triangle Business Journal, ... the Research Triangle region of North Carolina. , Just a ... roundtable include the role of social media in recruitment, ... and the unique role that Millennials play in the workplace ...
Breaking Biology Technology:Health Canada approves first near-patient DNA test for personalized medicine 2Health Canada approves first near-patient DNA test for personalized medicine 3Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3Regado Biosciences to Provide Third Quarter 2014 Financial Results 2Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 2Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 3
... , RESEARCH TRIANGLE PARK, N.C. , May 4 ... therapeutics, announced today that Kenneth I. Moch has ... Chimerix as Chief Operating Officer in July 2009 , ... and financing biomedical companies.  Mr. Moch will also join the ...
... May 4 PharmAthene, Inc. (NYSE Amex: PIP ), ... today announced that the Company,s Interim CEO, Eric I. Richman ... the critical interrelationship between government, academia and industry in the development ... , , ...
... Packing Record Tetrahedral dice, which have four ... yet tested, according to research performed by a collaboration ... revelation is the result of a series of experiments ... and adding more dice until the containers were completely ...
Cached Biology Technology:Chimerix Names George Painter, Ph.D., Chairman of the Board and Chief Scientific Officer; Appoints Kenneth I. Moch President and Chief Executive Officer 2Chimerix Names George Painter, Ph.D., Chairman of the Board and Chief Scientific Officer; Appoints Kenneth I. Moch President and Chief Executive Officer 3Chimerix Names George Painter, Ph.D., Chairman of the Board and Chief Scientific Officer; Appoints Kenneth I. Moch President and Chief Executive Officer 4PharmAthene CEO To Participate on Biosecurity Panel at the 2010 BIO International Conference 2Densest dice packing and computing with molecules 2
(Date:10/29/2014)... been blamed for increasing the risk of a ... history of schizophrenia, are widely accepted. Others, such ... parasite transmitted by soil, undercooked meat and cat ... new study by Gary Smith, professor of population ... School of Veterinary Medicine, used epidemiological modeling methods ...
(Date:10/29/2014)... EAST LANSING, Mich. – As bodies decompose, their ... Deciphering the clues they provide could mean the ... murder. , Michigan State University is using a ... to help Detroit death-scene investigators examine these changing ... such as geographical location of death, gender, race, ...
(Date:10/29/2014)... living world compete with members of their own ... they invest into their competitive ability. Some individuals ... to high-quality resources, while others seem to avoid ... resources that are left over for them. Moreover, ... societies seems to fluctuate considerably over time. A ...
Breaking Biology News(10 mins):Penn vet professor investigates parasite-schizophrenia connection 2Penn vet professor investigates parasite-schizophrenia connection 3MSU partners with Detroit to investigate death scenes 2Evolution of competitiveness 2Evolution of competitiveness 3
... 14, 2010 - Haze, scientifically known as atmospheric aerosols ... represents a major environmental problem because it degrades visibility, ... profound impacts, how the haze is formed is not ... atmospheric sciences and chemistry who has studied air chemistry ...
... as President Barack Obama called for in his address to ... all other companies be responsible to both their shareholders and ... Graduate School of Business. The rigorous survey-based nationwide ... the Gulf of Mexico found that Republicans, Democrats and Independents ...
... up to 70 percent of all women experience symptoms ... up to 13 percent of all new mothers suffer ... is defined as a major depressive episode starting within ... health problem. Postpartum blues represents a major risk factor ...
Cached Biology News:Research is getting closer to understanding critical nucleus in haze formation, prof says 2Consumer responses to Gulf oil spill reflect Americans' changing corporate expectations 2More than just the baby blues 2More than just the baby blues 3
Normal Hamster Serum...
... Short Stroke Thrust Slide is designed for ... envelopes are limited and substantial side-loading is ... bumpers provide millions of trouble-free cycles even ... composite bushings or recirculating ball bearings , ...
Alkaline Phosphatase (E. coli C75), 100 units. Removes 5'-phosphates from DNA. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzymes, Alkaline Phosphatases....
... Reactive Dye Pack, 12 x Cy5. ... optimized for microarray labeling.Higher reactivity Cy3 ... dye per vial, at > 75% ... dispensed, and packed in foil packs ...
Biology Products: