Navigation Links
Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

ash and spin
  • Add elution buffer and spin to collect DNA
  • Figure 1 outlines the simple and rapid protocols used for purifying miniprep plasmid DNA, PCR products and agarose gel-fractionated DNA. Each microspin cup in the StrataPrep plasmid miniprep kit yields up to 20 g of purified plasmid DNA from overnight bacterial cultures of 1.5 to 3 ml. Each microspin cup in the StrataPrep PCR purification kit and DNA gel extraction kit yields as much as 10 g of purified DNA.

    Plasmid Purification

    figure 2

    Plasmid DNA isolated with the StrataPrep plasmid miniprep kit exhibits OD260 -to-OD280 ratios between 1.8 and 2.0, indicating a high degree of nucleic acid purity.2 Starting with 30 individual 1.5- to 3-ml liquid cultures, purified plasmid DNA can be obtained in an average of 2.5 minutes per miniprep. Plasmid and cosmid DNAs isolated with the StrataPrep plasmid miniprep kit are easily digested with restriction enzymes (figure 2). Plasmid DNA purified with the StrataPrep kit transforms chemically competent E. coli with efficiencies equivalent to those obtained for DNA that has been purified using cesium chloride density gradients. For example, 100 pg of a 2.9-kb plasmid purified using either the StrataPrep kit or the cesium chloride density gradient method transformed Epicurian Coli XL2-Blue ultracompetent cells with an efficiency of 2 x 109 colony forming units (cfu)/g of plasmid DNA (data not shown).


    '"/>

    Source:


    Page: All 1 2 3 4 5 6

    Related biology technology :

    1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
    2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
    3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
    4. Simple Isolation of RNA from Tissue and Cultured Cells
    5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
    6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
    7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
    8. Simple Approaches for Optimization of RT-PCR
    9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
    10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
    11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
    Post Your Comments:
    (Date:5/5/2015)... Research and Markets ( http://www.researchandmarkets.com/research/4r95kr/clinical ... "Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services ... Europe Version" report to their offering. ... of molecular diagnostics and pharmacogenics hold the promise ... that is moving out of the national and ...
    (Date:5/5/2015)... 05, 2015 GEA Pharma Systems, ... as recipients of the INTERPHEX Exhibitor Awards for ... their Portable, Continuous, Miniature and Modular (PCMM) platform. ... footprint, continuous processing equipment, smart control systems and ... powders and inactive ingredients into bulk tablets. ...
    (Date:5/4/2015)... May 4, 2015 Tobira Therapeutics, Inc. ... the development and commercialization of novel treatments for ... of its merger with Regado Biosciences, Inc. (NASDAQ: ... closing of the merger, Tobira raised $40 million ... investors and all current Tobira institutional investors, including ...
    (Date:5/4/2015)... BROOK, N.Y. , May 4, 2015 /PRNewswire/ ... a $250,000 cooperative research and development agreement (CRADA) ... to advance the development of TRB-N0224 for the ... animals, including dogs and cats.  Traverse Biosciences has ... TRB-N0224 for the treatment and control of periodontal ...
    Breaking Biology Technology:European Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market 2015-2019 2GEA Pharma Systems, G-CON Manufacturing and Pfizer Consortium Receives Best Technology Innovation Award at INTERPHEX 2015 2GEA Pharma Systems, G-CON Manufacturing and Pfizer Consortium Receives Best Technology Innovation Award at INTERPHEX 2015 3Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 2Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 3Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 4Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 5Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 6Traverse Biosciences Executes Agreement with Aratana Therapeutics to Advance Development of TRB-N0224 for the Treatment and Control of Periodontal Disease in Pets 2Traverse Biosciences Executes Agreement with Aratana Therapeutics to Advance Development of TRB-N0224 for the Treatment and Control of Periodontal Disease in Pets 3Traverse Biosciences Executes Agreement with Aratana Therapeutics to Advance Development of TRB-N0224 for the Treatment and Control of Periodontal Disease in Pets 4
    ... Wis. - MyWeather LLC has named ... marketing officer. , ,Baird went to Carleton College and ... Wisconsin Governor Martin Schreiber. He has served as vice ... major K-12 technology company; vice president of sales and ...
    ... most prominent pharmaceutical companies has been purchased, its stock ... in a continued limited presence in Wisconsin, but could ... industry, increase distribution of their drug and has returned ... Corp. , a Massachusetts-based pharmaceutical firm, announced on Thursday ...
    ... When FedEx/Kinkos needed to dispose of 1,100 used ... a cost of $200,000 to the company and a ... Aidmatrix to essentially recycle the machines by donating them to ... , ,In case you ever wondered about what happened to ...
    Cached Biology Technology:Genzyme acquistion of Bone Care approved, significant impact forecast 2Genzyme acquistion of Bone Care approved, significant impact forecast 3Genzyme acquistion of Bone Care approved, significant impact forecast 4Internet charity link has a Wisconsin flavor in CEO Scott McCallum 2Internet charity link has a Wisconsin flavor in CEO Scott McCallum 3
    (Date:4/27/2015)... Apr. 27, 2015 NXT-ID, Inc. (NASDAQ: ... company focused on the growing mobile commerce market, announces ... pre-order customers the first week of May, 2015 and ... of May. Gino Pereira , ... for the company as Wocket® enters the consumer market. ...
    (Date:4/17/2015)... BURNABY, Canada , April 17, 2015 /PRNewswire/ ... projects, growing security concerns, and technological advancement to ... Arabia until 2020   ... " Saudi Arabia Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric ... is projected to register growth at CAGR ...
    (Date:4/10/2015)... -- Research and Markets ( http://www.researchandmarkets.com/research/cjk4gb/security ) ... Profiles - NEC" report to their offering. ... continue to supply a range of IT security management ... focus on the development of a Big Data and ... the Asia-Pacific region is a ...
    Breaking Biology News(10 mins):Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3NEC Security Competitive Profile 2015 2
    ... HEIDELBERG, 25 March 2013 Researchers have used ... are transmitted from animals to humans in two ... Denmark. The results, which are published today in ... of methicillin-resistant Staphylococcus aureus (MRSA), a ...
    ... poplars and willows are candidate "biofuel crops" from which ... content fuels can be efficiently extracted. Domesticating these ... and genetics of trees, and scientists are turning to ... peach and a poplar may not be obvious at ...
    ... August 2011, researchers from the U.S. Department of Agriculture ... puzzle in Kennewick, Wash. Since Kennewick lies within a ... apple-growing region, an annual survey of fruit trees is ... to look for any invading insects. This time the ...
    Cached Biology News:Sequencing tracks animal-to-human transmission of bacterial pathogens 2Peach genome offers insights into breeding strategies for biofuels crops 2Genetic analysis saves major apple-producing region of Washington state 2
    Ubiquitin Related I Sampler Kit 10 mu g each...
    DNA Repair Sampler Kit 10g each...
    PKC Sampler Kit 10 mu g each...
    ... phase reagent for Virus ... & unique water insoluble ... detergents and chaotropes; Engineered ... non-envelop viruses while preserving ...
    Biology Products: