Navigation Links
Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits


Jeff Braman Scott Basehore
Stratagene Cloning Systems, Inc.


Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid, PCR product and gel-fractionated DNAs. Purified miniprep DNA prepared with the StrataPrep plasmid miniprep kit is easily digested with restriction enzymes, is transformed with high efficiency and is accurately sequenced. With the StrataPrep PCR purification kit, PCR products are purified devoid of primers and small, nonspecific amplification products. These purified PCR products are cloned with high efficiency. Gel-isolated DNA that is purified using the StrataPrep DNA gel extraction kit is recovered with excellent yield and is efficiently cloned.

Purification of nucleic acids is a time-consuming part of many research projects. To eliminate this bottleneck, Stratagene has developed the StrataPrep nucleic acid purification kits. These products enable researchers to purify DNA from minipreps, PCR amplifications and agarose gels rapidly and with high yield and purity.

Nucleic acid purification using the StrataPrep products is based upon the principles discussed by Vogelstein and Gillespie.1 The kits utilize a special DNA-binding solid support that is manufactured in a microspin format. This format allows easy removal of contaminants by centrifugal washing, and the desired product is eluted in a small volume of buffer or water. The purified DNA is ready for a variety of applications.

Figure 1
Purification Protocols

'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
4. Simple Isolation of RNA from Tissue and Cultured Cells
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
8. Simple Approaches for Optimization of RT-PCR
9. Detection of Morphine and Morphine-Glucuronide in Saliva or Urine with a Rapid and Simple Method Without Derivatization on the Varian 500-MS
10. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
11. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
Post Your Comments:
(Date:3/27/2015)... The consumption of compound fertilizers (NPK) ... and high prices in 2008. However, the situation has ... on gaining momentum now. , To date, the world ... 100,000 thousand tonnes. No considerable growth was registered in ... rocketed during 2012-2013, registering 11% growth as against the ...
(Date:3/27/2015)... 27, 2015 Working in collaboration with ... London , Richmond Pharmacology is the first ... 3 study for an investigational RNAi therapeutic being developed for ... the nerves and heart. Read press release ... Based at St Georges University of London ...
(Date:3/26/2015)... March 26, 2015 PRC Clinical, a ... today several management and executive appointments effective immediately. , ... Officer (CFO) and member of the Board of Directors. ... Operations, and Sue Dowden will assume the position of ... current Chief Operations Officer (COO), has been appointed to ...
(Date:3/26/2015)... Mich., March 26, 2015 Neogen Corporation (NASDAQ: ... for the third quarter of fiscal 2015, which ended ... the prior year,s $6,575,000. Earnings per share in the ... ago. Current year-to-date net income increased 17% over prior ... $20,621,000, or $0.56 per share, for the same period ...
Breaking Biology Technology:World NPK Fertilizers Market Keeps On Gaining Momentum, According to BAC Reports Study Available at MarketPublishers.com 2World NPK Fertilizers Market Keeps On Gaining Momentum, According to BAC Reports Study Available at MarketPublishers.com 3PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3Neogen reports 13% increase in net income 2Neogen reports 13% increase in net income 3Neogen reports 13% increase in net income 4Neogen reports 13% increase in net income 5Neogen reports 13% increase in net income 6Neogen reports 13% increase in net income 7Neogen reports 13% increase in net income 8
... (NYSE: TMO ) announced that it will release ... the market opens on Thursday,October 25, 2007, and will ... 9:00,a.m. Eastern time. During the call, the company ... future expectations. To listen, call 866-802-4321,within the U.S. or ...
... Oct. 5 Iomai Corporation,(Nasdaq: IOMI ) today ... executive officer, will present at the Natixis,Bleichroeder Hidden Gems ... Erck,s presentation will begin at 2:45 p.m. Eastern Time ... To access the live audio webcast or the ...
... announced today that it has priced its initial public,offering ... of $12.00 per,share. All shares are being offered by ... a 30-day option to,purchase up to an additional 750,000 ... and Morgan Stanley & Co. Incorporated are joint,book-running managers ...
Cached Biology Technology:Thermo Fisher Scientific to Hold Earnings Conference Call on Thursday, October 25, 2007 2MAP Pharmaceuticals Announces Pricing of Its Initial Public Offering 2
(Date:3/12/2015)... 2015 IriTech, a leading iris based identity ... Texas Instruments Design Network, announced today that the Chief ... IriShield USB MK2120U device during a launching event of ... The iris scanner is manufactured by ... India through its Indian partner, ...
(Date:3/12/2015)... BERLIN , March 12, 2015 ... IAM, a standardized identity and access management (IAM) ... reliable implementation schedule. With Beta System,s new IAM ... and best practices gleaned from multiple IAM implementations ... includes the software as well as any necessary ...
(Date:3/11/2015)... DUBLIN , Mar. 11, 2015 Research and ... addition of the "Access Control Market by Product, ... to 2020" report to their offering. , ... is expected to reach $10.4 billion by 2020, with ... control market report covers the products types such as ...
Breaking Biology News(10 mins):Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2
... an invisible world teeming with microbes. Here, diverse species of ... which contribute to global warming. Now two Stanford University ... actually increase the production of two greenhouse gases nitrous ... gases to power the treatment plant. "Normally, we want ...
... by renowned conservationists such as David Attenborough and directors ... been awarded to Estella Leopold, a University of Washington ... The International Cosmos Prize carries a cash award ... just one individual or team each year, according information ...
... their lengthy annual migration to and from Mexico complete ... New research from the University of Guelph reveals that ... Lakes fly directly east over the Appalachians and settle along ... of monarchs migrated north directly from the Gulf coast. ...
Cached Biology News:Stanford engineers use rocket science to make wastewater treatment sustainable 2Stanford engineers use rocket science to make wastewater treatment sustainable 3Stanford engineers use rocket science to make wastewater treatment sustainable 4Stanford engineers use rocket science to make wastewater treatment sustainable 5University of Washington professor's lifetime efforts receive international recognition 2University of Washington professor's lifetime efforts receive international recognition 3U of G research reveals how monarchs fly away home 2
Shake 'n' Stack oven - New Model (includes 10 bottle capacity rotisserie, adjustable feet, drip tray, manual)...
... - Neurosphere is a system designed for ... neurospheres in a serum free environment. The kit ... which has been optimized for neural stem cell ... Genes & Development 10 :3129). Two growth ...
Request Info...
... RNase-Free DNase is a preparation of ... double-stranded DNA to produce 3-hydroxyl oligonucleotides. ... in applications where maintaining the integrity ... is qualified for use with the ...
Biology Products: