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Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications,,,and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acid purification kits


Jeff Braman Scott Basehore
Stratagene Cloning Systems, Inc.


Stratagene announces a new line of DNA purification products, which provide reliable isolation of high-purity miniprep plasmid, PCR product and gel-fractionated DNAs. Purified miniprep DNA prepared with the StrataPrep plasmid miniprep kit is easily digested with restriction enzymes, is transformed with high efficiency and is accurately sequenced. With the StrataPrep PCR purification kit, PCR products are purified devoid of primers and small, nonspecific amplification products. These purified PCR products are cloned with high efficiency. Gel-isolated DNA that is purified using the StrataPrep DNA gel extraction kit is recovered with excellent yield and is efficiently cloned.

Purification of nucleic acids is a time-consuming part of many research projects. To eliminate this bottleneck, Stratagene has developed the StrataPrep nucleic acid purification kits. These products enable researchers to purify DNA from minipreps, PCR amplifications and agarose gels rapidly and with high yield and purity.

Nucleic acid purification using the StrataPrep products is based upon the principles discussed by Vogelstein and Gillespie.1 The kits utilize a special DNA-binding solid support that is manufactured in a microspin format. This format allows easy removal of contaminants by centrifugal washing, and the desired product is eluted in a small volume of buffer or water. The purified DNA is ready for a variety of applications.

Figure 1
Purification Protocols

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