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Simple Isolation of RNA from Tissue and Cultured Cells

HL-60

13.1 0.62

2.1 0.01

3

Table 2 and Table 3 show yield and purity of RNA isolated from a variety of cells and tissues. The RNA exhibited A260/A280 ratios of greater than 1.9 for each sample, indicating high purity of the RNA. Approximately 1 g of representative RNA samples was electrophoresed on formaldehyde-agarose gels to assess the integrity of the RNA. Figure 2 shows intact 28S and 18S ribosomal RNA bands for each sample.

Fig.2

Table 3
Isolation of RNA from Various Mouse Tissues

RNA was isolated from different amounts of mouse tissues using the StrataPrep total RNA miniprep kit. Yield and purity were determined by measuring absorbance in 10 mM Tris, pH 7.5.

Tissue

Average yield (mg/mg tissue)

Average A260/A280

Number of isolations

Brain

0.7 0.14

2.2 0.12

4

Heart

0.3 0.06

2.2 0.21

2

Kidney
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