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Simple Isolation of RNA from Tissue and Cultured Cells

LIGN="LEFT">122 15.4

2.0 0.04

10

Table 1 includes yield and purity data for RNA isolated from varying amounts of rat liver, using the kit. The RNA yields were good, averaging 14 to 122 g for liver quantities between 5 and 40 mg; individual yields reached as high as 141 g. The purity, as determined by the A260/A280 ratio, was 1.9 or greater (Table 1).

Table 2
Isolation of RNA from Different Cell Lines

RNA was isolated from 2 x 106 cells using the StrataPrep total RNA miniprep kit. Yield and purity were determined by measuring absorbance in 10 mM Tris, pH 7.5.

Species

Cell line

Average yield (mg/2 x 106 cells)

Average A260/A280

Number of
isolations

Mouse

NIH/3T3

41 6.2

2.1 0.07

8

Hamster

CHO

25.0 0.75

2.2 0.04

3

Human
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