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Simple Isolation of RNA from Tissue and Cultured Cells

a wide range of starting materials, both tissues and cells. We then assessed the quality of the isolated RNA by absorbance measurements, gel electrophoresis, RT-PCR, and molecular beacon real-time RT-PCR.

RNA Isolation from Different Quantities and Sources of Tissue and Cells

Table 1
RNA Yield and Purity from Varying Amounts of Rat Liver

RNA was isolated from the specified amounts of tissue using the StrataPrep total RNA miniprep kit. Yield and purity were determined by measuring absorbance in 10 mM Tris, pH 7.5.

Amount of rat liver

Average yield (g)

Average A260/A280

Number ofisolations

5 mg

14 0.8

2.1 0.07

3

10 mg

26 10.1

2.1 0.06

7

20 mg

51 14.1

2.0 0.09

11

30 mg

94 11.4

2.0 0.02

6

40 mg

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