Navigation Links
Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

ugated antibody will be formed. This complex will bind to a specific anti-mouse isotype antibody that is immobilized on the membrane. The appearance of a pink band on the IsoDetect isotyping membrane at a defined location indicates the specific isotype of the mouse immunoglobulin tested. The sensitivity of the test is 0.1 g/ml of mouse immunoglobulin, and test results are determined visually within 5 minutes (figure 1). The IsoDetect kit is sensitive, simple, highly specific and requires no equipment. Unlike other isotyping products, the IsoDetect kit requires only a simple dilution of ascites or culture media with the provided phosphate buffered saline before applying the sample to the IsoDetect card. No agitation, mixing of reagents or long incubation periods are necessary. The IsoDetect card is completely self-contained and is ideally formatted for archiving permanent records. For added convenience, Stratagene is also offering the IsoDetect isotyping control kit, which contains purified mouse immunoglobulin isotype standards. The IsoDetect control kit can be used as an adjunct to the IsoDetect kit or can be used for other applications, such as dot blot assays.

Conclusions

Stratagenes IsoDetect mouse monoclonal antibody isotyping kit is a rapid method for determining isotypes of mouse monoclonal antibodies from tissue culture and ascites samples. The method uses a colloidal-gold conjugate technique to achieve sensitivities of 0.1 g/ml in 5 minutes. In addition, Stratagene is offering the IsoDetect isotyping control kit, which is a series of standards that can be used with the IsoDetect kit or for other isotyping applications.

REFERENCES

  1. Harlowe, E., and Lane, D. (1988) Antibodies: A
    '"/>



Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
5. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
6. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
7. Highly Sensitive microRNA Array Performance
8. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
10. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
11. Mouse Tail Genomic DNA Isolation Protocol(1)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Simple Sensitive Isotyping Mouse Monoclonal Antibodies

(Date:7/11/2014)... -- EPFL and the University of  Geneva ... John P. Donoghue . The American neuroscientist is to ... The new Wyss Center for Bio- and ... , has named as its director one of ... , founder of Brown University,s Institute for Brain Science, ...
(Date:7/10/2014)... 2014 Product and process impurities ... abundance and are often “lost in the noise,” ... Join presenters Dr. Rowel Tobias, Senior Scientist, Protein ... Head of Quality at Nanotherapeutics, Inc., to lear ... speed detection and quantitation while achieving the required ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Robert Harman, DVM, ... Medicine company, is proud to announce the relaunch of his ... with a new series called “ What are Stem Cells ... an honest and straightforward foundation in the basics of stem ... decision on the right type of treatment when considering regenerative ...
(Date:7/10/2014)... July 10, 2014 On July ... Medvedev , presented Russia,s first ... . The Company is developing a unique project called ... BIOCAD develops a number of innovative drugs based on ... The ceremony took place at the International Exhibition "Innoprom ...
Breaking Biology Technology:John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2
... Novavax, Inc. (Nasdaq: NVAX ) a clinical-stage vaccine company, ... Annual Health Care Conference 2009 on December 2, 2009 at 12:30 ... Novavax will provide an overview of the company,s business strategy, research ... live audio only presentation can be accessed via the Company website ...
... 30 Dr. Jessica Torrente, Assistant Professor of Radiology ... demonstrated the integration and use of Breast-Specific Gamma Imaging ... for the diagnosis of breast cancer today at the ... (RSNA). , "The purpose of the exhibit was to ...
... 30 BioMarin Pharmaceutical Inc. (Nasdaq: BMRN ) ... has granted orphan drug designation for 3,4-diaminopyridine (3,4-DAP), amifampridine ... Syndrome (LEMS). 3,4-DAP has previously received orphan drug ... the Committee for Medicinal Products for Human Use of ...
Cached Biology Technology:NOVAVAX to Present at the Piper Jaffray 21st Annual Health Care Conference 2Breast-Specific Gamma Imaging In Clinical Practice as an Adjunct Imaging Modality for Diagnosis of Breast Cancer 2FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 2FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 3FDA Grants Orphan Drug Designation for 3,4-DAP for LEMS 4
(Date:7/11/2014)... international team of researchers, including scientists from the Max ... a reported a major step in understanding photosynthesis, the ... maintains the oxygen in its atmosphere and which is ... earth. , The researchers report the first direct ... namely the step in which a specific protein complex, ...
(Date:7/11/2014)... of kidney injury related to the use of hydroxyethyl ... HES molecules, according to a report in Anesthesia ... Research Society (IARS). , The "total mass of HES ... human renal proximal tubule cells (PTCs), concludes the laboratory ... Hospital Wrzburg, Austria. Other factorssuch as differences in ...
(Date:7/11/2014)... normal circumstances, astrocytes participate in normal physiological ... exhibit therapeutic and repairing effects on brain ... found that nerve cells differentiated from adipose-derived ... viability, which produces influences on subsequent studies ... Hospital, Hebei United University, China demonstrated that ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... in the equatorial Pacific by the end of the century, ... study shows that climate change could cause ocean currents to ... of islands right on the equator. Those islands include ... of Kiribati. This low-lying country is at risk from sea-level ...
... New research published online in the FASEB ... 5-lipoxygenase, plays a key role in cell death induced ... likely help prevent or lessen the severity of immune ... space conditions initiate health problems that mimic the aging ...
... going on in DPR Korea than rocket science: local people ... their food supply through agroforestry. This is according to a ... How participatory agroforestry restored land and secured the food supply ... that in DPR Korea a bottomup participatory process of developing ...
Cached Biology News:Global warming refuge discovered near at-risk Pacific island nation of Kiribati 2Global warming refuge discovered near at-risk Pacific island nation of Kiribati 3Global warming refuge discovered near at-risk Pacific island nation of Kiribati 4Scientists discover enzyme that could slow part of the aging process in astronauts -- and the elderly 2Agroforestry is not rocket science but it might save DPR Korea 2Agroforestry is not rocket science but it might save DPR Korea 3
... derivatives that have mutations in both the ... genes, which greatly enhances disulfide bond formation ... expression in Origami(DE3) yielded 10-fold more active ... overall expression levels were similar (1). Origami ...
... Blunt TOPO PCR Cloning ... designed for cloning and ... PCR products. The pCR4Blunt-TOPO ... Cloning and greater than ...
... CELLection Dynabeads (4.5 m) coated with the ... EpCAM and DNase Releasing Buffer. For ... tumour cells directly from blood, bone marrow ... enriched from bone marrow. Enriched cells are ...
Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera)...
Biology Products: